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tdTomato-β-actin3'UTR1

Plasmid

#183716

Purpose

tdTomato with fragment nucleotides 1-440 of beta-actin 3'UTR inserted in 3'UTR

Depositor

Jeffrey Gerst

Article

Dasgupta et al Elife. 2023 May 30;12:e83584. doi: 10.75

Insert

tdTomato with fragment (nucleotides 1-440) of mouse Actb 3'UTR

Use

Lentiviral

Tags

Expression

Mutation

Promoter

UbiC

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-sg-tdTomato166/892

Plasmid

#179919

Purpose

Double cutting Cas9 plasmid with puromycin resistance and a single guide RNA targeting tdTomato fluorescent protein sites 166 and 892.

Depositor

Indeever Madireddy, Johan Sosa

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

tdTomato sgRNA

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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ymTq2-tdTomato pDRF1-GW

Plasmid

#168068

Purpose

FRET fusion between ymTq2 and tdTomato for use in yeast

Depositor

Bas Teusink

Insert

ymTq2-tdTomato

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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pBS-f1(-)-TOMM20-dTomato

Plasmid

#115922

Purpose

donor to insert dTomato at the TOMM20 locus in human cells

Depositor

Erik Sontheimer

Article

Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

Insert

TOMM20-dTomato

Use

Donor template

Tags

dTomato

Expression

Mutation

Promoter

Available since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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pBS-f1(+)-SEC61B-dTomato

Plasmid

#115913

Purpose

donor to insert dTomato at the SEC61B locus in human cells

Depositor

Erik Sontheimer

Article

Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

Insert

SEC61B-dTomato (SEC61B Human)

Use

Donor template

Tags

dTomato

Expression

Mutation

Promoter

Available since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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pBS-f1(+)-TOMM20-dTomato

Plasmid

#115914

Purpose

donor to insert dTomato at the TOMM20 locus in human cells

Depositor

Erik Sontheimer

Article

Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

Insert

TOMM20-dTomato (TOMM20 Human)

Use

Donor template

Tags

dTomato

Expression

Mutation

Promoter

Available since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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YSBE415: pTOP_ACA1_Vc_HOG1 terminator

Plasmid

#122434

Purpose

Plasmid for tagging proteins in Cryptococcus neoformans; C-terminal fragment of Venus protein

Depositor

Yong-Sun Bahn

Article

So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

Insert

C-terminal fragment of Venus protein

Use

Tagging proteins in cryptococcus neoformans

Tags

Expression

Mutation

Promoter

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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YSBE420:pNEO_CAC1_Vn_HOG1 terminator

Plasmid

#122701

Purpose

Plasmid for tagging proteins in Cryptococcus neoformans; N-terminal fragment of Venus protein

Depositor

Yong-Sun Bahn

Article

So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

Insert

N-terminal fragment of Venus protein

Use

Tagging proteins in cryptococcus neoformans

Tags

Expression

Mutation

Promoter

Available since

May 1, 2019

Availability

Academic Institutions and Nonprofits only

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YSBE416: pTOP_CAC1_Vn_HOG1 terminator

Plasmid

#122435

Purpose

Plasmid for tagging proteins in Cryptococcus neoformans; N-terminal fragment of Venus protein

Depositor

Yong-Sun Bahn

Article

So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

Insert

N-terminal fragment of Venus protein

Use

Tagging proteins in cryptococcus neoformans

Tags

Expression

Mutation

Promoter

Available since

May 1, 2019

Availability

Academic Institutions and Nonprofits only

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YSBE418:pNAT_ACA1_Vc_HOG1 terminator

Plasmid

#122700

Purpose

Plasmid for tagging proteins in Cryptococcus neoformans; C-terminal fragment of Venus protein

Depositor

Yong-Sun Bahn

Article

So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

Insert

C-terminal fragment of Venus protein

Use

Tagging proteins in cryptococcus neoformans

Tags

Expression

Mutation

Promoter

Available since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-Atoh1 del3

Plasmid

#96913

Purpose

Murine Atoh1 with amino acids 214-305 removed

Depositor

Albert Edge

Article

Cheng et al J Biol Chem. 2016 Sep 30;291(40):21096-2

Insert

Atoh1 (Atoh1 Mouse)

Use

Tags

2xFLAG

Expression

Mammalian

Mutation

Deletion of amino acids 214-305

Promoter

Available since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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FLAG-Atoh1 del4

Plasmid

#96914

Purpose

Murine Atoh1 with amino acids 306-347 removed

Depositor

Albert Edge

Article

Cheng et al J Biol Chem. 2016 Sep 30;291(40):21096-2

Insert

Atoh1 (Atoh1 Mouse)

Use

Tags

2xFLAG

Expression

Mammalian

Mutation

Deletion of amino acids 306-347

Promoter

Available since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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FLAG-Atoh1 S334A

Plasmid

#96915

Purpose

Murine Atoh1 with S334A mutation

Depositor

Albert Edge

Article

Cheng et al J Biol Chem. 2016 Sep 30;291(40):21096-2

Insert

Atoh1 (Atoh1 Mouse)

Use

Tags

2xFLAG

Expression

Mammalian

Mutation

Serine 334 changed to Alanine

Promoter

Available since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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FLAG-Atoh1 S309A

Plasmid

#96916

Purpose

Murine Atoh1 with S309A mutation

Depositor

Albert Edge

Article

Cheng et al J Biol Chem. 2016 Sep 30;291(40):21096-2

Insert

Atoh1 (Atoh1 Mouse)

Use

Tags

2xFLAG

Expression

Mammalian

Mutation

Serine 309 changed to Alanine

Promoter

Available since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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FLAG-Atoh1 S325A

Plasmid

#96926

Purpose

Murine Atoh1 with S325A mutation

Depositor

Albert Edge

Article

Cheng et al J Biol Chem. 2016 Sep 30;291(40):21096-2

Insert

Atoh1 (Atoh1 Mouse)

Use

Tags

2xFLAG

Expression

Mammalian

Mutation

Serine 325 changed to Alanine

Promoter

Available since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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FLAG-Atoh1 S328A

Plasmid

#96927

Purpose

Murine Atoh1 with S328A mutation

Depositor

Albert Edge

Article

Cheng et al J Biol Chem. 2016 Sep 30;291(40):21096-2

Insert

Atoh1 (Atoh1 Mouse)

Use

Tags

2xFLAG

Expression

Mammalian

Mutation

Serine 328 changed to Alanine

Promoter

Available since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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FLAG-Atoh1 S331A

Plasmid

#96928

Purpose

Murine Atoh1 with S331A mutation

Depositor

Albert Edge

Article

Cheng et al J Biol Chem. 2016 Sep 30;291(40):21096-2

Insert

Atoh1 (Atoh1 Mouse)

Use

Tags

2xFLAG

Expression

Mammalian

Mutation

Serine 331 changed to Alanine

Promoter

Available since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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FLAG-Atoh1 S339A

Plasmid

#96929

Purpose

Murine Atoh1 with S339A mutation

Depositor

Albert Edge

Article

Cheng et al J Biol Chem. 2016 Sep 30;291(40):21096-2

Insert

Atoh1 (Atoh1 Mouse)

Use

Tags

2xFLAG

Expression

Mammalian

Mutation

Serine 339 changed to Alanine

Promoter

Available since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pAdx-EF1-tdTomato

Plasmid

#73354

Purpose

Express tdTomato under EF1 promoter

Depositor

Kazuhiro Oka

Insert

EF1-tdTomato

Use

Adenoviral

Tags

Expression

Mutation

Promoter

EF-1

Available since

July 1, 2016

Availability

Academic Institutions and Nonprofits only

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pCS-Fv2Eflt4um200-2a-tdTomato

Plasmid

#67698

Purpose

Expression construct

Depositor

Nathan Lawson

Article

Shin et al Development. 2016 Oct 15;143(20):3785-37

Insert

Fv2E-flt4-2A-tdTomato (flt4 Zebrafish)

Use

Tags

2A-tdTomato and HA

Expression

Mutation

um200

Promoter

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only