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Plasmid#27162DepositorInsertMKL1 (MRTFA Human)
UseTags3XFLAGExpressionMammalianMutationPromoterAvailable sinceDec. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
TIRAP-TAP_IRES_GFP
Plasmid#52740PurposeRetroviral vector expresses hTIRAP-3xHA and a target site for the biotin ligase BirA upstream of IRES and GFPDepositorInsertToll/IL-1 Receptor adaptor protein (TIRAP Human)
UseRetroviralTags3x HA, 3x TEV, and BirA target siteExpressionMammalianMutationPromoterAvailable sinceJuly 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
PX-TIR-GFP
Plasmid#52737PurposeRetroviral vector expresses the lipid binding domain of p40-phox fused to the TIR of hTIRAP and GFP. Also contains IRES-hCD2 as selectable markerDepositorInsertToll/IL-1 Receptor adaptor protein (TIRAP Human)
UseRetroviralTagsGFP and p40 Phox lipid binding domainExpressionMammalianMutationDeleted amino acids 1-85PromoterAvailable sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
PLC-TIR-GFP
Plasmid#52736PurposeRetroviral vector expresses the lipid binding domain of PLCd1 fused to the TIR of hTIRAP and GFP. Also contains IRES-hCD2 as selectable markerDepositorInsertToll/IL-1 Receptor adaptor protein (TIRAP Human)
UseRetroviralTagsGFP and PLCd1 Lipid bindingExpressionMammalianMutationDeleted amino acids 1-85PromoterAvailable sinceJune 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
SLP2a-TIR-GFP
Plasmid#52738PurposeRetroviral vector expresses the lipid binding domain of SLP2a fused to the TIR of hTIRAP and GFP. Also contains IRES-hCD2 as selectable markerDepositorInsertToll/IL-1 Receptor adaptor protein (TIRAP Human)
UseRetroviralTagsC2A domain of Murine SLP2a and GFPExpressionMammalianMutationDeleted amino acids 1-85PromoterAvailable sinceJune 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pADH143
Plasmid#90989PurposeNAT-marked "empty" gRNA expression construct; part 2 of 2 of C.mal LEUpOUT CRISPR system. Use with pADH140 CAS9 expression construct.DepositorInsertNAT 2 of 2, pSNR52, empty gRNA, gRNA conserved, C. mal LEU2 2 of 2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pADH139
Plasmid#90987PurposeNAT-marked "empty" gRNA construct for use in gRNA expression cassette stitching PCR; use with pADH110 to generate target-specific part 2 of 2 for C.mal LEUpOUT CRISPR system.DepositorInsertgRNA conserved, C, mal LEU2 2 of 2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-mTagBFP2-MRTF-A
Plasmid#182061PurposeMammalian expression vector encoding myocardin-related transcription factor A tagged with mTagBFP2.DepositorInsertmTagBFP2-MRTF-A (MRTFA Human)
UseTagsmTagBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-MKL1
Plasmid#11978PurposeFor mammalian expression of human MKL1 with a 3x FLAG tagDepositorInsertMKL1 (MRTFA Human)
UseTags3xFLAGExpressionMammalianMutationN579S T716APromoterCMVAvailable sinceAug. 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
p3XFLAG-MKL1-~100
Plasmid#27176DepositorInsertMKL1 (MRTFA Human)
UseTags3XFLAGExpressionMammalianMutationDeletion of 100 aa from ATGPromoterAvailable sinceDec. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCHA_SBV_NSs
Plasmid#208938PurposeExpresses C-terminally HA-tagged Schmallenberg orthobunyavirus NSs protein from CMV promoter in mammalian cellsDepositorInsertNSs
UseTagsHAExpressionMammalianMutationDeleted 47 bp at 5' end and 510 bp from 3…PromoterCMVAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a MDHP_YEAST
Plasmid#204233PurposeBacterial expression of yeast peroxisomal malate dehydrogenase ; Use in CURES (integration of research into undergraduate teaching labs).InsertMDHP_YEAST (MDH3 Budding Yeast)
UseTags6X HisExpressionBacterialMutationThe last 3 C-terminal residues (SKL) are the pero…PromoterLacAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pAcUW21-hovimeh1
Plasmid#84211Purposebaculovirus transfer vector containing hovimeh1 coding sequence from Homalodisca vitripennisDepositorInsertmeh1
UseTagsExpressionInsectMutationPromoterp10Available sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pCMS-eGFP-RTP801
Plasmid#65057PurposeExpression of RTP801 in mammalian cellsDepositorInsertDDIT4 (Ddit4 Rat)
UseTagsEGFPExpressionMammalianMutationPromoterCMV-IEAvailable sinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
EF1α-mNeonGreen-Z1
Plasmid#135613PurposemNeonGreen monomeric fluorescent protein expression for mammalian cells under EF1a-HTLV promoter in minimal backbone plasmid.DepositorInsertmNeonGreen
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1a-HTLVAvailable sinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
EF1α-eGFP-Z1
Plasmid#135612PurposeEnhanced green fluorescent protein expression for mammalian cells under EF1a-HTLV promoter in minimal backbone plasmid.DepositorInserteGFP
UseTagsExpressionMammalianMutationPromoterEF1a-HTLVAvailable sinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
CAG-eGFP-Z1
Plasmid#135046PurposeEnhanced Green fluorescent protein under constitutive CAG Promoter for mammalian expression in minimal backbone plasmidDepositorInserteGFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCAG PromoterAvailable sinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only