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Showing: 381 - 400 of 3885 results

  1. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-EGFP

    Plasmid
    #118156
    Purpose
    Catalytically inactive Cas9 from S. pyogenes with P2A-EGFP under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-P2A-EGFP
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a core and U6
    Available since
    April 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    118156-plasmid-map-sequence-id-239644

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  2. lenti-UCOE-dCas9-BFP-Zim3-KRAB

    Plasmid
    #196716
    Purpose
    CRISPR-inhibition. dCas9-KRAB(ZIM3) with TagBFP for sorting. Improved KRAB domain from ZIM3
    Depositor
    Bernhard Schmierer
    Insert
    dCas9-TagBFP-KRAB(ZIM3)
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Expression
    Mutation
    D10A, N863A
    Promoter
    EF1a
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196716-plasmid-map-sequence-id-388040

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  9. pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196084
    Purpose
    (Empty Backbone) Inducible CRISPRi vector conferring puromycin resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Joanna Bereta
    Article
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Promoter
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    196084-plasmid-map-sequence-id-388993

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  10. pHR-EF1a-dCas9-HA-BFP-KRAB-NLS

    Plasmid
    #102244
    Purpose
    Lentiviral expression of dCas9-HA-BFP-KRAB-NLS for CRISPRi driven by a hEF1alpha promoter
    Depositor
    Jacob Corn
    Insert
    dCas9-HA-BFP-KRAB-NLS
    Use
    CRISPR and Lentiviral
    Tags
    HA-TagBFP-KRAB-NLS
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    102244-plasmid-map-sequence-id-197289

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  11. zcUAS:PSD95.FingR-GFP-ZFC(CCR5TC)-KRAB(A)

    Plasmid
    #72638
    Purpose
    Tol2 based UAS modified with a CCR5TC binding site upstream of it, driving PSD95-FingR fused to GFP, CCR5TC zinc finger, and KRAB(A) repressor domain
    Depositor
    Joshua Bonkowsky
    Insert
    UAS upstream of PSD95-FingR with ZFC(CCR5TC) and KRAB(A)
    Use
    Gateway
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    72638-plasmid-map-sequence-id-445453

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  12. pLenti-TO/CMV-puro-DD-PAmCherry1-KRas G12D

    Plasmid
    #177320
    Purpose
    Lentiviral vector expressing PAmCherry1-tagged KRas 4B G12D mutant, with an N-terminal dimerization domain (DD).
    Depositor
    Xiaolin Nan, Steven Chu
    Article
    Nan et al Proc Natl Acad Sci U S A. 2015 Jun 30;11
    Insert
    KRAS 4B (KRAS Human)
    Use
    Lentiviral
    Tags
    FKBP-derived dimerization domain (DD, or DmrB) an…
    Expression
    Mammalian
    Mutation
    G12D
    Promoter
    TetOn-CMV
    Available since
    Jan. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    177320-plasmid-map-sequence-id-353281

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  14. pTol2-ziUAS:GPHN.FingR-mCherry-ZFI (IL2RGTC)-KRAB(A)

    Plasmid
    #72639
    Purpose
    Tol2 based UAS modified with a IL2RGTC binding site upstream of it, driving GPHN-FingR fused to mCherry, IL2RGTC zinc finger, and KRAB(A) repressor domain
    Depositor
    Joshua Bonkowsky
    Insert
    UAS upstream of GPHN-FingR with ZFI(IL2RFTC) and KRAB(A)
    Use
    Gateway
    Tags
    Expression
    Mutation
    Stop codon after mCherry- SEE DEPOSITOR COMMENTS …
    Promoter
    Available since
    April 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    72639-plasmid-map-sequence-id-429021

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  17. pSBbi-Hyg-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196076
    Purpose
    (Empty Backbone) Constitutive CRISPRi vector conferring hygromycin B resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Joanna Bereta
    Article
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Promoter
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    196076-plasmid-map-sequence-id-388979

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  19. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR

    Plasmid
    #118155
    Purpose
    Catalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the hPGK promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Jorge Ferrer
    Article
    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41
    Insert
    KRAB-dCas9-P2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    hPGK and U6
    Available since
    May 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    118155-plasmid-map-sequence-id-243704

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  20. pSBtet-Hyg-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196082
    Purpose
    (Empty Backbone) Inducible CRISPRi vector conferring hygromycin B resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Joanna Bereta
    Article
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Aminoglycoside phosphotransferase
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Promoter
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    196082-plasmid-map-sequence-id-388978

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Showing: 381 - 400 of 3885 results