Addgene

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p{CFD4-EYFP-3xP3::DsRed}

Plasmid

#86863

Purpose

Expresses an EYFP gRNA ubiquitously and DsRed in eyes as a marker. With attB integration site.

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2017 Apr 3;7(4):1339-1347

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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AAV-syn-ChR2-EYFP-Kv

Plasmid

#89256

Purpose

Channelrhodopsin localized to neuronal soma via a Kv2.1 targeting motif

Depositor

McLean Bolton

Article

Baker et al Elife. 2016 Aug 15;5. pii: e14193. doi:

Insert

Channelrhodopsin

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

H134R

Promoter

synapsin

Availability

Academic Institutions and Nonprofits only

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pLenti-CaMKIIa-ChETA-EYFP

Plasmid

#26967

Purpose

3rd gen lentiviral expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by CaMKIIa promoter for optogenetic activation

Depositor

Karl Deisseroth

Article

Gunaydin et al Nat Neurosci. 2010 Mar;13(3):387-92. doi

Insert

hChR2(E123T/H134R)-EYFP

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Mutation

E123T

Promoter

CaMKIIa

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO EYFP

Plasmid

#27056

Depositor

Karl Deisseroth

Has Service

AAV Retrograde, AAV1, AAV2, AAV5, and AAV9

Insert

EYFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-C1V1 (t/t)-TS-EYFP

Plasmid

#35499

Purpose

AAV expression of CaMKII-driven chimeric opsin variant C1V1 (E122T/E162T) for fast and potent optical excitation at red-shifted wavelengths.

Depositor

Karl Deisseroth

Article

Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

Has Service

AAV9

Insert

ChR1-VChR1 Chimera

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

E122T and E162T

Promoter

CaMKIIa

Availability

Academic Institutions and Nonprofits only

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pLenti-CaMKIIa-C1C2-EYFP

Plasmid

#35519

Depositor

Karl Deisseroth

Article

Kato et al Nature. 2012 Jan 22. doi: 10.1038/nature

Insert

ChR1-ChR2 Chimera

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

CaMKIIa

Availability

Academic Institutions and Nonprofits only

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pLenti-CaMKIIa-C1C2-TS-EYFP

Plasmid

#35520

Depositor

Karl Deisseroth

Article

Kato et al Nature. 2012 Jan 22. doi: 10.1038/nature

Insert

ChR1-ChR2 Chimera

Use

Lentiviral

Tags

Trafficking signal and EYFP

Expression

Mammalian

Mutation

Promoter

CaMKIIa

Availability

Academic Institutions and Nonprofits only

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pTRE-TIGHT-Cx43-eYFP

Plasmid

#31807

Depositor

Robin Shaw

Article

Smyth et al J Clin Invest. 2010 Jan 4. 120(1):266-79

Insert

Cx43 (GJA1 Human)

Use

Tags

eYFP

Expression

Mammalian

Mutation

Promoter

P-TIGHT

Availability

Academic Institutions and Nonprofits only
pENTR-R4r-EYFP-R3r

Plasmid

#32412

Depositor

Matthew Nolan

Article

White et al Front Mol Neurosci. 2011;4:8. Epub 2011

Insert

EYFP

Use

Gateway cloning vector

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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LCMV:ECFP(loxP)(FRT)EYFP

Plasmid

#31304

Depositor

Gerhart Ryffel

Article

Ryffel et al Nucleic Acids Res. 2003 Apr 15. 31(8):e4

Insert

ECFP(loxP)(FRT)EYFP

Use

Cre/Lox; Flp/frt

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pPHR-EYFP-NLS-VP16

Plasmid

#103822

Purpose

Soluble BLInCR transcription activator (transactivation domain of viral VP16 protein) that is recruited to 'localizer' sites upon blue light illumination

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

PHR-EYFP-VP16 (CRY2 Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

VP16: G126T (silent, G42G)

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pPHR-EYFP-hGCN5-NLS

Plasmid

#103827

Purpose

Soluble BLInCR effector that is recruited to 'localizer' sites upon blue light illumination

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

PHR-EYFP-hGCN5-NLS (CRY2, KAT2A Human, Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

hGCN5: A566G (E189G), G585T (K195N), G726A (silen…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-DIO-EYFP

Plasmid

#104052

Purpose

An AAV genome encoding Cre-dependent expression of the fluorescent protein EYFP from the CAG promoter

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Has Service

AAV PHP.V1

Insert

EYFP

Use

AAV

Tags

Expression

Mutation

Promoter

CAG

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-eYFP-f

Plasmid

#104057

Purpose

An AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein eYFP with a C-terminal Hras farnesylation sequence

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Insert

EYFP-f

Use

AAV

Tags

Expression

Mutation

Promoter

TRE

Availability

Academic Institutions and Nonprofits only

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pBS-TRE-EYFP-WPRE

Plasmid

#104104

Purpose

Expresses EYFP under the TRE promoter in mammalian cells.

Depositor

Takeshi Imai

Article

Sakaguchi et al Elife. 2018 Nov 20;7. pii: 40350. doi: 1

Insert

EYFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-EYFP-WPRE

Plasmid

#104111

Purpose

AAV-mediated expression of EYFP under the TRE promoter.

Depositor

Takeshi Imai

Article

Sakaguchi et al Elife. 2018 Nov 20;7. pii: 40350. doi: 1

Insert

EYFP

Use

AAV

Tags

Expression

Mutation

Promoter

TRE

Availability

Academic Institutions and Nonprofits only

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pCAG-hChR2(H134R)-EYFP

Plasmid

#114367

Purpose

expresses channelrhodopsin 2 fused with EYFP

Depositor

Mingshan Xue

Article

Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

Insert

hChR2(H134R)

Use

Tags

EYFP

Expression

Mammalian

Mutation

the Histidine at amino acid position 134 was chan…

Promoter

pCAG

Availability

Academic Institutions and Nonprofits only

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pcDNA5-eYFP-FKBP-MAD1-WT

Plasmid

#114037

Purpose

Chemical induction of MAD1 localisation to a specific site in the cell (by rapamycin & FRB)

Depositor

Geert Kops

Article

Kuijt et al Chromosoma. 2014 Oct;123(5):471-80. doi:

Insert

Human MAD1 (MAD1L1 Human)

Use

Tags

eYFP-FKBP12

Expression

Mammalian

Mutation

none

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA5-eYFP-FKBP-MAD1-AA

Plasmid

#114038

Purpose

Chemical induction of MAD1 localisation to a specific site in the cell (by rapamycin & FRB)

Depositor

Geert Kops

Article

Kuijt et al Chromosoma. 2014 Oct;123(5):471-80. doi:

Insert

Human MAD1 (MAD1L1 Human)

Use

Tags

eYFP-FKBP12

Expression

Mammalian

Mutation

MAD2 binding deficient MAD1 K541A/L543A

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-sbGLuc-B7-EYFP

Plasmid

#114110

Purpose

fusion protein of Gaussia luciferase variant slow burn, B7 transmembrane region, and enhanced yellow fluorescent protein; control for bioluminescent optogenetics

Depositor

Ute Hochgeschwender

Article

Gomez-Ramirez et al J Neurosci Res. 2020 Mar;98(3):471-480.

Inserts

Gaussia luciferase, slow burn
B7 hinge region
Enhanced Yellow Fluorescent Protein

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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