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pIRHD-TetR-GAL80-Rho1-GAL4

Plasmid

#218274

Purpose

Introduction of low-temperature-inducible GAL system, LowTempGAL v2.2

Depositor

Christopher Howard

Article

Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

Insert

gal80Arm1-pAgTEF1>KanMX4>tAgTEF1-pHAC1>UBI4>>DHFRP66L-TetR>NLS>TUP1>tABF1-pTEF2>Rho1C17R>>GAL4>tGAL4-pTEF1(TetO)>GAL80Arm2

Use

Tags

Expression

Yeast

Mutation

DHFR (P66L), Rho1 (C17R)

Promoter

Available since

May 24, 2024

Availability

Academic Institutions and Nonprofits only

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pIRHDGAL43C

Plasmid

#218279

Purpose

Introduction of a LowTempGAL Turbo module (HD-GAL43C) to accelerate induction of GAL promoter induction

Depositor

Claudia Vickers

Article

Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

Insert

fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pNRG2>GAL4>tNRG2-PENO2> UBI4>RHGSGTMV>DHFRP66L>G*6>GAL3C(S509P)-tENO2-fcy1(454-778)

Use

Tags

Expression

Yeast

Mutation

GAL3 (S509P), DHFR (P66L)

Promoter

Available since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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pIRHDGAL3C

Plasmid

#218276

Purpose

Introduction of a LowTempGAL Turbo module (HD-GAL3C) to accelerate induction of GAL promoter induction

Depositor

Claudia Vickers

Article

Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

Insert

fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pENO2> UBI4>RHGSGTMV>DHFRP66L>G*6>GAL3C(S509P)>tENO2-fcy1(454-778)

Use

Tags

Expression

Yeast

Mutation

GAL3 (S509P), DHFR (P66L)

Promoter

Available since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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pERB257

Plasmid

#67761

Purpose

Expresses KLC1-mCherry-eDHFR

Depositor

Michael Lampson

Article

Ballister et al Curr Biol. 2015 May 18;25(10):R407-8. do

Insert

KLC1-mCherry-eDHFR (Klc1 Rat)

Use

Tags

eDHFR and mCherry

Expression

Mammalian

Mutation

contains Klc1 amino acids 1-175

Promoter

CAG

Available since

Jan. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pTRE-8XGTIIC-DsRED-DD

Plasmid

#115798

Purpose

lentiviral, trimethoprim (TMP) regulated YAP promoter activity reporter

Depositor

Joan Massague

Article

Er et al Nat Cell Biol. 2018 Aug;20(8):966-978. d

Insert

YAP responsive promoter (YAP1 Human)

Use

Lentiviral

Tags

DsRED-DHFR

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pERB260

Plasmid

#67763

Purpose

Expresses BICD-mCherry-eDHFR

Depositor

Michael Lampson

Article

Ballister et al Curr Biol. 2015 May 18;25(10):R407-8. do

Insert

BICD-mCherry-eDHFR

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pDA12

Plasmid

#113663

Purpose

Cloning vector for bacterial expression with Pdhfr promoter

Depositor

Miguel Valvano

Article

Aubert et al Infect Immun. 2008 May;76(5):1979-91. do

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Pdhfr

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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GOMO-GFP-LUC

Plasmid

#60976

Purpose

Rapid selection of recombinant marker-free bioluminescent rodent malaria parasites

Depositor

Olivier Silvie

Article

Manzoni et al Sci Rep. 2014 Apr 23;4:4760. doi: 10.103

Inserts

GFP-Luciferase fusion
mCherry
hDHFR-yFCU

Use

Tags

Expression

Mutation

Promoter

Available since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

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GOMO-GFP

Plasmid

#60975

Purpose

Rapid selection of recombinant marker-free fluorescent rodent malaria parasites

Depositor

Olivier Silvie

Article

Manzoni et al Sci Rep. 2014 Apr 23;4:4760. doi: 10.103

Inserts

GFP
mCherry
hDHFR-yFCU

Use

Plasmodium gene targeting

Tags

Expression

Mutation

Promoter

Available since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

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pET51b(+)-NADPH-LUCID-4 (C50 6.5 uM)

Plasmid

#117911

Purpose

Plasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 6.5 uM

Depositor

Kai Johnsson

Article

Yu et al Science. 2018 Sep 14;361(6407):1122-1126

Insert

SNAP_Pro30_[cpNLuc23_24]eDHFR(R44A-H45Q)

Use

Tags

His-tag and Strep-tag

Expression

Bacterial

Mutation

R44A & H45Q on eDHFR

Promoter

T7

Available since

Oct. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pET51b(+)-NADPH-LUCID-3 (C50 1.4 uM)

Plasmid

#117910

Purpose

Plasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 1.4 uM

Depositor

Kai Johnsson

Article

Yu et al Science. 2018 Sep 14;361(6407):1122-1126

Insert

SNAP_Pro30_[cpNLuc23_24]eDHFR(R44A)

Use

Tags

His-tag and Strep-tag

Expression

Bacterial

Mutation

R44A on eDHFR

Promoter

T7

Available since

Oct. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pET51b(+)-NADPH-LUCID-2 (C50 29 nM)

Plasmid

#117909

Purpose

Plasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 29 nM

Depositor

Kai Johnsson

Article

Yu et al Science. 2018 Sep 14;361(6407):1122-1126

Insert

SNAP_Pro30_[cpNLuc23_24]eDHFR(R98A)

Use

Tags

His-tag and Strep-tag

Expression

Bacterial

Mutation

R98A on eDHFR

Promoter

T7

Available since

Oct. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pFTP1

Plasmid

#64952

Purpose

source of TpR FRT cassette

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Insert

FRT-dhfrII-FRT

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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10xUAS-GFP-DD

Plasmid

#104989

Purpose

Expresses GFP fused with destabilizing domain (DD) downstream of a 10XUAS sequence.

Depositor

Jing-W Wang

Article

Sethi et al Elife. 2017 Nov 15;6. pii: e30327. doi:

Insert

TMP-inducible destabilized GFP (GFP-DD)

Use

Tags

DD

Expression

Insect

Mutation

Promoter

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pDAI-SceI

Plasmid

#32061

Purpose

Construction of unmarked gene deletions. Broad host range replicative plasmid expressing the I-SceI homing endonuclease

Depositor

Miguel Valvano

Article

Flannagan et al Environ Microbiol. 2008 Jun;10(6):1652-6

Insert

I-SceI nuclease

Use

Tags

Expression

Bacterial

Mutation

Promoter

Pdhfr

Available since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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10XUAS-flp-DD

Plasmid

#124777

Purpose

Expresses flippase fused with destabilizing domain (DD) downstream of a 10XUAS sequence.

Depositor

Jing-W Wang

Article

Sethi et al Elife. 2017 Nov 15;6. pii: e30327. doi:

Insert

TMP-inducible destabilized FLP (FLP-DD)

Use

Tags

DD

Expression

Insect

Mutation

Promoter

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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nSYB-GAL80-DD

Plasmid

#124778

Purpose

Expresses GAL80 fused with destabilizing domain (DD) downstream of a n-Synaptobrevin promoter

Depositor

Jing-W Wang

Article

Sethi et al Elife. 2017 Nov 15;6. pii: e30327. doi:

Insert

TMP-inducible destabilized GAL80 (GAL80-DD)

Use

Tags

DD

Expression

Insect

Mutation

Promoter

nSynaptobrevin

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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PL6-CS-WR

Plasmid

#122512

Purpose

construction for sgRNA transcription with Plasmodium falciparum U6 promotor

Depositor

Lubin Jiang

Article

Xiao et al Proc Natl Acad Sci U S A. 2019 Jan 2;116

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

PfU6

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pET51b(+)_LAMA-F98 (for GFP)

Plasmid

#130714

Purpose

Plasmid encoding the bacterial expression of LAMA-F98 for GFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

LAMA-F98

Use

Tags

Hisx10

Expression

Bacterial

Mutation

Deleted first Met, Ala and Last Ser from the Enha…

Promoter

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3.0_mitoLAMA-F98

Plasmid

#130704

Purpose

Plasmid encoding outer mitochondrial localization of LAMA-F98 for GFP as a SNAP-Tag-fusion

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

NTOM20-SNAP-Tag-LAMA-F98

Use

Tags

NTOM20 and SNAP-Tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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