Addgene

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pMCB619

Plasmid

#171011

Purpose

CRISPR sgRNA vector (puro-BFP with BstXI/BlpI digest sites).

Depositor

Alice Ting

Article

Coukos et al Elife. 2021 Aug 20;10. pii: 69142. doi:

Insert

U6:sgRNA_Puro-T2A-BFP

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6

Available since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pLX304-YAP1(S94A)

Plasmid

#59145

Purpose

YAP1 serine 94 o alanine mutants which disrupts binding to TEAD transcription factors

Depositor

William Hahn

Article

Shao et al Cell. 2014 Jul 3;158(1):171-84. doi: 10.

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Tags

Expression

Mutation

S94A

Promoter

CMV

Available since

Aug. 18, 2014

Availability

Academic Institutions and Nonprofits only
pCW57.1-Tet-UPF1WT (pRKB264)

Plasmid

#99146

Purpose

Inducible lentiviral construct to express FLAG-tagged UPF1wt

Depositor

Robert Bradley

Article

Feng et al Mol Cell. 2017 Jul 20;67(2):239-251.e6.

Insert

UPF1 (UPF1 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

First 127 codons are optimized to reduce GC conte…

Promoter

pTight

Available since

Nov. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pHH0103_GNA14_G-alpha

Plasmid

#109914

Purpose

Protein expression and purification of GNA14_G-alpha

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

GNA14_G-alpha (GNA14 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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IFNAR2_pCSdest

Plasmid

#53914

Purpose

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

IFNAR2 (IFNAR2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SP6 and CMV

Available since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
pEntry_HCoV-NL63-nsp5

Plasmid

#168914

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-NL63-nsp5 (ORF1ab )

Use

Gateway-compatible entry vector

Tags

Expression

Mutation

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pUB6-CDC34-HA (pRKB261)

Plasmid

#99145

Purpose

Mammalian expression construct to express HA-tagged CDC34

Depositor

Robert Bradley

Article

Feng et al Mol Cell. 2017 Jul 20;67(2):239-251.e6.

Insert

CDC34 (CDC34 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

pTight

Available since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR-p38KTRClover

Plasmid

#59142

Purpose

ORF encoding p38 KTR mClover flanked by Gateway sequences

Depositor

Markus Covert

Article

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

p38 Kinase Translocation Reporter (MAPK14 Human, Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pQCXIH BRGI-Flag

Plasmid

#19148

Depositor

Joan Massague

Article

Xi et al J Biol Chem. 2008 Jan 11. 283(2):1146-55

Insert

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 15, 2008

Availability

Academic Institutions and Nonprofits only
EGFP-uTEV1-NES

Plasmid

#171006

Purpose

HiLITR protease with C-terminal NNES (Cytoplasmic)

Depositor

Alice Ting

Article

Coukos et al Elife. 2021 Aug 20;10. pii: 69142. doi:

Insert

EGFP-uTEV1-NES

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

pTRE-tight (TetOn)

Available since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pLX304-YAP1 _PDZ

Plasmid

#59147

Purpose

YAP1 mutants that harbored a deletion of the 5 amino acids

Depositor

William Hahn

Article

Shao et al Cell. 2014 Jul 3;158(1):171-84. doi: 10.

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

PDZ binding domain (last 5aa) deleted

Promoter

CMV

Available since

Oct. 23, 2014

Availability

Academic Institutions and Nonprofits only
p-EGFP-C1-Flag-mFmr1(D)

Plasmid

#87914

Purpose

Expresses mouse FMRP-EGFP fusion with phosphorylation mimic S499D.

Depositor

Stephanie Ceman

Article

Stetler et al Hum Mol Genet. 2006 Jan 1;15(1):87-96. E

Insert

Fmr1 (Fmr1 Mouse)

Use

Tags

Flag and eGFP

Expression

Mammalian

Mutation

S499D

Promoter

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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EGFP-uTEV1-CB5

Plasmid

#171007

Purpose

HiLITR protease with CB5 C-terminal tmd targeting information (ER)

Depositor

Alice Ting

Article

Coukos et al Elife. 2021 Aug 20;10. pii: 69142. doi:

Insert

EGFP-uTEV1-CB5(tmd)

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

pTRE-tight (TetOn)

Available since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-uTEV1-MAVS

Plasmid

#170998

Purpose

HiLITR protease with MAVS c-terminal tmd targeting information (Mitochondria)

Depositor

Alice Ting

Article

Coukos et al Elife. 2021 Aug 20;10. pii: 69142. doi:

Insert

EGFP-uTEV1-MAVS(tmd)

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

pTRE-tight (TetOn)

Available since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-SARS-CoV-2 SL1 alone-Luciferase

Plasmid

#191482

Purpose

Luciferase reporter for SARS-CoV-2 5' UTR SL1 alone

Depositor

Hao Wu

Article

Shi et al bioRxiv. 2020 Sep 18. doi: 10.1101/2020.

Insert

SARS-CoV-2 5' UTR SL1 alone

Use

Lentiviral and Luciferase

Tags

Expression

Mutation

Contains only the stem-loop1 region of SARS-CoV-2…

Promoter

Available since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-uTEV1-SQStmd

Plasmid

#171009

Purpose

HiLITR protease with SQS C-terminal targeting information (ER)

Depositor

Alice Ting

Article

Coukos et al Elife. 2021 Aug 20;10. pii: 69142. doi:

Insert

EGFP-uTEV1-SQS(tmd)

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

pTRE-tight (TetOn)

Available since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-uTEV1-ACBD5

Plasmid

#171008

Purpose

HiLITR protease with ACBD5 C-terminal targeting information (peroxisome)

Depositor

Alice Ting

Article

Coukos et al Elife. 2021 Aug 20;10. pii: 69142. doi:

Insert

EGFP-uTEV1-ACBD5(tmd)

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

pTRE-tight (TetOn)

Available since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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TOP2A G11.2 gRNA

Plasmid

#90914

Purpose

3rd generation lentiviral gRNA plasmid targeting human TOP2A

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

TOP2A (Guide Designation G11.2)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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mRuby2-Tubulin-6

Plasmid

#55914

Purpose

Localization: Microtubules, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

Tubulin (TUBA1B Human)

Use

Tags

mRuby2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pPB-CAG-mNanog-mut-pgk-hph

Plasmid

#74914

Purpose

Mammalian expression of mNanog (Y42E, K43T)

Depositor

Jose Silva

Article

Theunissen et al Development. 2011 Nov;138(22):4853-65. d

Insert

Nanog (Nanog Mouse)

Use

Tags

Expression

Mammalian

Mutation

K43T of Nanog protein and Y42E of Nanog homeodoma…

Promoter

CAG

Available since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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