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Lenti-EF1alpha-VP64-dCas9-p300_Blast

Plasmid

#192654

Purpose

3rd generation lenti vector encoding VP64-dCas9-p300 with 2A Blast resistance marker

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

VP64-dCas9-p300

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

D10A; H840A in Cas9

Promoter

EF1a

Available since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pHH0103_PRKACA_C1+C2

Plasmid

#110027

Purpose

Protein expression and purification of PRKACA_C1+C2

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

PRKACA_C1+C2 (PRKACA Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pZR064_CROPseq-sgCD4-CRISPRi

Plasmid

#180270

Purpose

Lentiviral CROPseq vector expressing CD4 targeting sgRNA for CRISPRi

Depositor

Alexander Marson

Article

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Insert

CD4 sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT/TO Plk4 KD-mCherry

Plasmid

#80270

Purpose

mammalian expression plasmid for kinase dead Plk4

Depositor

Andrew Holland

Article

Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

Insert

Plk4 (PLK4 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

D154A kinase dead

Promoter

CMV

Available since

Aug. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA5-hygro-eGFP-HEC1

Plasmid

#114027

Purpose

Inducible expression of eGFP-tagged HEC1 in FLPin Trex cell lines

Depositor

Geert Kops

Article

Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

Insert

eGFP-HEC1 - Wildtype (NDC80) (NDC80 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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iRFP713-FRB-PIP5K1A

Plasmid

#202743

Purpose

expresses recruitable fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

iRFP713:MTOR(2021-2113):GGSA2:PIP5K1A (PIP5K1A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCA-G-intron-G

Plasmid

#40027

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

GFP exon 1
GFP exon 2
beta-globin intron

Use

Tags

Expression

Mammalian

Mutation

insertion of a loxP site, nucleotides 1-274, and …

Promoter

CAG (chicken beta globin promoter and CMV enhance…

Available since

Oct. 12, 2012

Availability

Academic Institutions and Nonprofits only

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iRFP713-FRB-Mss4-Kina

Plasmid

#202744

Purpose

expresses recruitable fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

iRFP713:MTOR(2021-2113):GGSA2:S. cerevisiae Mss4 (377-756) (MSS4 Budding Yeast)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMOD_A0908

Plasmid

#91027

Purpose

Module A, Promoter: EC1.2, Gene: TREX2-P2A-AtCas9, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

TREX2-P2A-AtCas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

EC1.2

Available since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pBlue-loxPuro-APEX-PML_LASA

Plasmid

#140273

Purpose

a targeting plasmid for APEX-PML

Depositor

Yusuke Miyanari

Article

Kurihara et al Mol Cell. 2020 May 7;78(3):493-505.e8. d

Insert

APEX2

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

endogenous

Available since

May 1, 2020

Availability

Academic Institutions and Nonprofits only

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PH-PLCd1-mNGx3

Plasmid

#202753

Purpose

expresses fluorescent protein-tagged lipid biosensor

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

PLCD1v2(1-170): mNG2:mNG2:mNG2 (PLCD1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pHW-CIBN-MP-HFW-CRY2-VHH(GFP)

Plasmid

#126027

Purpose

express Flag-tagged LARIAT for optogenetic clustering

Depositor

Eurico Morais-de-Sá

Article

Osswald et al Biomolecules. 2019 Feb 12;9(2). pii: bio

Insert

CIBN-MP; CRY2 PHR-VHH(GFP)

Use

Tags

Flag

Expression

Insect

Mutation

WT

Promoter

Hsp70

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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prLCA-Citrine

Plasmid

#40271

Depositor

Martin Offterdinger

Article

Offterdinger et al Traffic. 2008 Jan;9(1):147-55. Epub 2007

Insert

Clta (Clta Rat)

Use

Tags

mCitrine

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 26, 2012

Availability

Academic Institutions and Nonprofits only

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Or30a-Gal4

Plasmid

#63027

Purpose

To express Gal4 in the pattern of the Drosophila melanogaster odorant receptor Or30a, using the promoter sequences of the gene cloned 5' of Gal4

Depositor

Leslie Vosshall

Article

Fishilevich et al Curr Biol. 2005 Dec 6;15(23):2086-96.

Insert

Drosophila melanogaster Or30a promoter

Use

Tags

Drosophila ADH (aa's 1-18) fused to Yeast Ga…

Expression

Insect

Mutation

Promoter

Available since

April 22, 2015

Availability

Academic Institutions and Nonprofits only
pFUPI

Plasmid

#80279

Purpose

A bicistronic lentiviral vector used to stably express cDNAs.

Depositor

Jeremy Luban

Article

Neagu et al J Clin Invest. 2009 Oct;119(10):3035-47.

Insert

Puro resistance cassette, IRES, cloning site

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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pmyc-GFP-TNRC6C-(928-1020)-NES-mut

Plasmid

#42027

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6C (TNRC6C Human)

Use

Tags

EGFP and Myc

Expression

Mammalian

Mutation

contain aa928-1020, L943A, L947A, M950A, F952A

Promoter

CMV

Available since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

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pDEST14-Cys-SpyCatcher (Min)

Plasmid

#216713

Purpose

Expresses truncated minimal form of SpyCatcher with free cysteine for labeling in bacteria. See also Li...Howarth, J Mol. Biol., 2014

Depositor

Alex Dunn

Article

Zhong et al Cell Rep Methods. 2022 Sep 2;2(9):100278

Insert

Cys-SpyCatcher (Min)

Use

Tags

6xHis

Expression

Bacterial

Mutation

See Li...Howarth, J. Mol. Biol., 2014

Promoter

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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PCUP1-Vac8-GFP-Ura

Plasmid

#207027

Purpose

Expression of Vac8-GFP under CPU1 promoter.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

Vac8

Use

Tags

GFP

Expression

Yeast

Mutation

Promoter

pCUP1

Available since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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Salsa-BC186-P2H6

Plasmid

#203027

Purpose

Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC186

Depositor

Noah Palm

Article

Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

Insert

EGFP/BC186

Use

Tags

Expression

Mammalian

Mutation

Promoter

Tet Response Element (TRE)

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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mt SSB

Plasmid

#205057

Purpose

SSB1 protein expression

Depositor

Smita Patel

Article

Johnson et al J Biol Chem. 2023 Jan;299(1):102797. doi

Insert

SSB1 (SSB Human)

Use

Tags

Expression

Bacterial

Mutation

WT

Promoter

Available since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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