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Plasmid#103874PurposeBroad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Kluyveromyces lactisDepositorInsertHH-gRNA-HDV targetting ADE2 in Kluyveromyces lactis
UseCRISPRTagsExpressionYeastMutationPromoterScTDH3Available sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
MAP2K1 gRNA (BRDN0001147078)
Plasmid#76211Purpose3rd generation lentiviral gRNA plasmid targeting human MAP2K1DepositorInsertMAP2K1 (MAP2K1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MAP2K1 gRNA (BRDN0001147218)
Plasmid#76212Purpose3rd generation lentiviral gRNA plasmid targeting human MAP2K1DepositorInsertMAP2K1 (MAP2K1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pU6-pegRNA-SNCA-A30P
Plasmid#180016PurposeTransiently expressing a pegRNA to introduce SNCA-A30P mutation in human cellsDepositorInsertPrime editing pegRNA for SNCA-A30P (SNCA Synthetic)
UseCRISPRTagsExpressionMammalianMutationPromoterHuman U6Available sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
HIPK2 gRNA (BRDN0001162222)
Plasmid#76750Purpose3rd generation lentiviral gRNA plasmid targeting human HIPK2DepositorInsertHIPK2 (HIPK2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2 kif26b sgRNA1
Plasmid#102857PurposeExpresses sgRNA targeting mouse Kif26bDepositorInsertMouse Kif26b (Kif26b Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
JAK3 gRNA (BRDN0001149525)
Plasmid#77188Purpose3rd generation lentiviral gRNA plasmid targeting human JAK3DepositorInsertJAK3 (JAK3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
Rab11 single guide RNA
Plasmid#229683Purposeguide RNA for Rab11 tagging at the N-terminus in pX459DepositorInsertRab11 guide (RAB11A Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
LMPd Amt PHGDH
Plasmid#209409PurposeRetroviral vector with Ametrine marker for expressing shRNA with an "UltramiR" microRNA scaffoldDepositorInsertPhgdh shRNA (Phgdh Mouse)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(ID1_5-2)-PGKpuroBFP-W
Plasmid#211964PurposeExpress gRNA against ID1 with puro and BFPDepositorInsertsgRNA targeting ID1 (ID1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterHuman U6 promoterAvailable sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(RUNX1(11))-PGKpuro2ABFP-W
Plasmid#200500PurposeLentiviral vector expressing gRNA targeting human RUNX1DepositorInsertRUNX1(11) (RUNX1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT2/shCol1a1/Scarlet_Seq1.3
Plasmid#206137PurposeKnockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.DepositorInsertCOL1A1 (Col1a1 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT2/shCol1a1/GFP4_Seq1.3
Plasmid#206136PurposeKnockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.DepositorInsertCOL1A1 (Col1a1 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti KO CLDN1 2xgRNA - spCas9 iRFP670 puro
Plasmid#166133PurposeThis plasmid allows efficient KO of the cldn1 gene, while expressing the far red fluorescent protein iRFP670 and puromycin resistanceDepositorInserthU6-gRNA and hH1-gRNA targeting cldn1 gene
UseCRISPR and LentiviralTagsiRFP670ExpressionMammalianMutationPromoterAvailable sinceApril 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-9
Plasmid#129037Purposetargeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA9 targeting human TSDRDepositorInsertdCas9-huTET1CD, SgRNA9 (huTSDR)
UseTagsHA-Tag, NLS and T2A-mCherryExpressionMammalianMutationdCas9 (D10A;H840A)PromoterCbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)Available sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(gKAT6A-A7)-PGKpuro2ABFP-W
Plasmid#159289PurposeLentiviral vector expressing gRNA targeting KAT6A (gRNA ID. A7)DepositorInsertguide RNA targeting KAT6A (ID. A7) (KAT6A Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhuman U6 promoterAvailable sinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
AOI-WT-Cas9-sg-mouse Gfi1-exon4(F2)-GFP
Plasmid#91877PurposeExpresses 3xFLAG-Cas9 and a gRNA targeting mouse Gfi1DepositorInsertgRNA targeting Gfi1 (Gfi1 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
AOI-WT-Cas9-sg-mouse Gfi1-exon4(F1)-GFP
Plasmid#91883PurposeExpresses 3xFLAG-Cas9 and a gRNA targeting mouse Gfi1DepositorInsertgRNA targeting Gfi1 (Gfi1 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
ACVRL1 gRNA (BRDN0001149004)
Plasmid#77781Purpose3rd generation lentiviral gRNA plasmid targeting human ACVRL1DepositorInsertACVRL1 (ACVRL1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
DAPK3 gRNA (BRDN0001162354)
Plasmid#77556Purpose3rd generation lentiviral gRNA plasmid targeting human DAPK3DepositorInsertDAPK3 (DAPK3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
DAPK3 gRNA (BRDN0001147205)
Plasmid#77558Purpose3rd generation lentiviral gRNA plasmid targeting human DAPK3DepositorInsertDAPK3 (DAPK3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
ITPKA gRNA (BRDN0001149513)
Plasmid#76857Purpose3rd generation lentiviral gRNA plasmid targeting human ITPKADepositorInsertITPKA (ITPKA Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
AK3 gRNA (BRDN0001147565)
Plasmid#76583Purpose3rd generation lentiviral gRNA plasmid targeting human AK3DepositorInsertAK3 (AK3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
GAK gRNA (BRDN0001148048)
Plasmid#76472Purpose3rd generation lentiviral gRNA plasmid targeting human GAKDepositorInsertGAK (GAK Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
TKFC gRNA (BRDN0001148963)
Plasmid#75785Purpose3rd generation lentiviral gRNA plasmid targeting human TKFCDepositorInsertTKFC (DAK Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
BMPR1A gRNA (BRDN0001147849)
Plasmid#77959Purpose3rd generation lentiviral gRNA plasmid targeting human BMPR1ADepositorInsertBMPR1A (BMPR1A Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
Tet-pLKO-puro-Scrambled
Plasmid#47541PurposeTet inducible scrambled shRNA.DepositorInsertScrambled shRNA
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterH1Available sinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pORANGE GFP-CACNA1A KI
Plasmid#131480PurposeEndogenous tagging of CaV2.1, P/Q: N-terminal (amino acid position: G10)DepositorUseTagsExpressionMammalianMutationPromoterU6 and CbhAvailable sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pORANGE GFP-Grin1 KI
Plasmid#131485PurposeEndogenous tagging of GluN1: N-terminal (amino acid position: A20)DepositorUseTagsExpressionMammalianMutationPromoterU6 and CbhAvailable sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT2-shATRX
Plasmid#124258PurposeExpresses shRNA targeting ATRX. Construct has inverted repeats to be used in Sleeping beauty system.DepositorInsertshATRX
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 8, 2019AvailabilityAcademic Institutions and Nonprofits only