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Plasmid#135267PurposeGateway destination clone of CDK13 (human) tagged with N-terminal TurboID-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertTurboID-V5-CDK13 (CDK13 Human)
UseLentiviral; Gateway destinationTagsV5-TurboIDExpressionMammalianMutationPromoterUbiquitinAvailable sinceJan. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1V5-His Snk/Plk2 T239A
Plasmid#22219DepositorInsertSnk/Plk2 (PLK2 Human)
UseTagsExpressionMammalianMutationsingle amino acid substitution mutant, threonine …PromoterAvailable sinceAug. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
HyGCaMP6s
Plasmid#102558PurposeExpression of GCaMP6s in HydraDepositorInsertGCaMP6s
UseHydra expressionTagsActin/GCaMP6s fusion protein (C terminal on backb…ExpressionMutationPromoterAvailable sinceOct. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pTRANS_210d
Plasmid#91109PurposeTransformation, Type: T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: noneDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoter35SAvailable sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCG_SARS-CoV2 ORF8 C-V5-tag
Plasmid#179990PurposeMammalian expression vector for SARS-CoV-2 ORF8, V5-tagged. Sequence codon-optimized.DepositorInsertSARS-CoV-2 ORF8 (ORF8 Synthetic)
UseTagsExpressionMammalianMutationhuman codon-optimizedPromoterAvailable sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAMBIA_ASX
Plasmid#98888PurposePlant expression vector for multigene cloningDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionPlantMutationPromoterCaMV35S (enhanced)Available sinceJan. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSB1A3-AD011
Plasmid#116852PurposeExpresses araC constitutively and mScarletI (RFP) under a pBAD promoter; alternate name pSB1A2-AD011DepositorInsertsAraC
mScarletI
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpBADAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
PAM2
Plasmid#198741PurposepGAPDH-GFP, Geneticin resistanceDepositorInsertGFP
UseAcanthamoeba expressionTagsExpressionMutationPromoterpGAPDHAvailable sinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.SF-iGluSnFR.A184V
Plasmid#106199PurposeMedium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-iGluSnFR.A184V
UseAAVTagsExpressionMutationGltI: A184VPromoterCAGAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-EGFP-ALDH1A1-Neo
Plasmid#189742PurposeThe construct was used to express GFP tagged human ALDH1A1 in mammalian cells for the analysis of the subcellular location of this protein.DepositorInsertALDH1A1 (ALDH1A1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterPGKAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCSIIhyg-RGECO1.0
Plasmid#175813PurposeThe lentiviral vector for R-GECO1.0, a genetically encoded red fluorescent Ca2+ sensor.DepositorInsertRGECO1.0
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHACK-GAL4>QF2(newHA1)
Plasmid#104873PurposeHACK Donor plasmids for converting GAL4 lines to QF2 lines using HACK method. Updated version of Addgene#80275 for easier cloning of insertsDepositorInsertT2A-QF2
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceMarch 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBrain-ch-TOGKDP-GFP-shch-TOG
Plasmid#69113PurposeDual-promoter plasmid to express knockdown-proof human ch-TOG tagged with EGFP along with shRNA to knock down endogenous ch-TOG in mammalian cells.DepositorInsertsUseRNAiTagsEGFPExpressionMammalianMutationSilent mutations to confer shRNA resistance.PromoterCMVAvailable sinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
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EF1a_ESRRG_P2A_Hygro_Barcode
Plasmid#120435PurposeBarcoded lentiviral vector to express ESRRG in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertESRRG (ESRRG Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
GluN2A (px330:2-guideCas9)
Plasmid#169428PurposeExpression of Cas9 and 2 guidesDepositorInsertspCas9
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
t1-435
Plasmid#166578PurposeFor expression of human talin head (residues 1-435) in E. coli. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.DepositorInsertTln1 Head (1-435) (TLN1 Human)
UseTagsHis6-tag, Xpress-epitope (DLYDDDDK) and enterokin…ExpressionBacterialMutationresidues 1-435 onlyPromoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
Banshee RunxDN mCherry
Plasmid#80158Purposeretroviral expression of Runx1DN and mCherryDepositorInsertRunx1 (Runx1 Mouse)
UseRetroviralTagsHA and IRES-mCherryExpressionMammalianMutationDN--truncated version of the Runx1 protein contai…PromoterAvailable sinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
mCherry-AuroraAKD-C-7
Plasmid#54998PurposeLocalization: Centrosomes, Excitation: 587, Emission: 610DepositorInsertAuroraA KD (AURKA Human)
UseTagsmCherryExpressionMammalianMutationKinase Dead - I57V, K162RPromoterCMVAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only