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Plasmid#147823PurposeMammalian Expression of HsUpf1-T28A-siRNAresDepositorInsertHsUpf1-T28A-siRNAres (UPF1 Human)
UseTagsExpressionMammalianMutation5 non silent mutations A69S, E167G, K282R, A442G…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo-lambdaN-UPF1-DEAA
Plasmid#136007PurposeDEAA UPF1 inserted with lambdaN tagged on the N-terminus to use with the Tethering Assay (BoxB)DepositorInsertUPF1
UseTagsLambdaNExpressionMammalianMutationDE647AA and S743A (Please see depositor comments …PromoterAvailable sinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT-TO-EGFP-UPF1-R615A
Plasmid#136001PurposeDOX-inducible R615A UPF1 inserted with GFP tagged on the N-terminus and used with the Flp-In T-Rex SystemDepositorInsertUPF1
UseTagsEGFPExpressionMammalianMutationR615APromoterAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT-TO-EGFP-UPF1-DEAA
Plasmid#136002PurposeDOX-inducible DEAA UPF1 inserted with GFP tagged on the N-terminus and used with the Flp-In T-Rex SystemDepositorInsertUPF1
UseTagsEGFPExpressionMammalianMutationDE647AAPromoterAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT7-V5-SBP-C1-HsUpf1-del119-272-siRNAres_P
Plasmid#147194PurposeMammalian Expression of HsUpf1-del119-272-siRNAresDepositorInsertHsUpf1-del119-272-siRNAres (UPF1 Human)
UseTagsExpressionMammalianMutation4 non silent mutations A69S, K282R, A442G and T1…PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-Tet-UPF1S124A/N138A/T139A (pRKB268)
Plasmid#99144PurposeInducible lentiviral construct to express FLAG-tagged UPF1S124A/N138A/T139ADepositorInsertUPF1 (UPF1 Human)
UseLentiviralTagsFlagExpressionMammalianMutationFirst 127 codons are optimized to reduce GC conte…PromoterpTightAvailable sinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT7-V5-SBP-C1-HsUpf1_115-1129-S1107A-siRNAres_S
Plasmid#147477PurposeMammalian Expression of HsUpf1_115-1129-S1107A-siRNAresDepositorInsertHsUpf1_115-1129-S1107A-siRNAres (UPF1 Human)
UseTagsExpressionMammalianMutation4 non silent mutations E167G, K282R, A442G and T1…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmUPF1_1-896-siRNAres-V5His6_N
Plasmid#146984PurposeInsect Expression of DmUPF1_1-896-siRNAresDepositorInsertDmUPF1_1-896-siRNAres (Upf1 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmUPF1_1-1106-siRNAres-V5His6_N
Plasmid#146985PurposeInsect Expression of DmUPF1_1-1106-siRNAresDepositorInsertDmUPF1_1-1106-siRNAres (Upf1 Fly)
UseTagsExpressionInsectMutation1 non silent mutations: G995E compared to the seq…PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmUPF1_1-1142-siRNAres-V5His6_N
Plasmid#146986PurposeInsect Expression of DmUPF1_1-1142-siRNAresDepositorInsertDmUPF1_1-1142-siRNAres (Upf1 Fly)
UseTagsExpressionInsectMutation1 non silent mutations: G995E compared to the seq…PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmUPF1_92-896-siRNAres-V5His6_N
Plasmid#146987PurposeInsect Expression of DmUPF1_92-896-siRNAresDepositorInsertDmUPF1_92-896-siRNAres (Upf1 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmUPF1_92-1106-siRNAres-V5His6_N
Plasmid#146988PurposeInsect Expression of DmUPF1_92-1106-siRNAresDepositorInsertDmUPF1_92-1106-siRNAres (Upf1 Fly)
UseTagsExpressionInsectMutation1 non silent mutations: G995E compared to the seq…PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmUPF1_92-1142-siRNAres-V5His6_N
Plasmid#146989PurposeInsect Expression of DmUPF1_92-1142-siRNAresDepositorInsertDmUPF1_92-1142-siRNAres (Upf1 Fly)
UseTagsExpressionInsectMutation1 non silent mutations: G995E compared to the seq…PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmUPF1-K479Q-siRNAres-V5His6_N
Plasmid#146991PurposeInsect Expression of DmUPF1-K479Q-siRNAresDepositorInsertDmUPF1-K479Q-siRNAres (Upf1 Fly)
UseTagsExpressionInsectMutation1 non silent mutations: G995E compared to the seq…PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmUPF1-S1121A-siRNAres-V5His6_N
Plasmid#146992PurposeInsect Expression of DmUPF1-S1121A-siRNAresDepositorInsertDmUPF1-S1121A-siRNAres (Upf1 Fly)
UseTagsExpressionInsectMutation1 non silent mutations: G995E compared to the seq…PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmUPF1-S1140AS1146AS1121A-siRNAres-V5His6_N
Plasmid#146993PurposeInsect Expression of DmUPF1-S1140AS1146AS1121A-siRNAresDepositorInsertDmUPF1-S1140AS1146AS1121A-siRNAres (Upf1 Fly)
UseTagsExpressionInsectMutation1 non silent mutations: G995E compared to the seq…PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmUPF1-S1140AS1146A-siRNAres-V5His6_N
Plasmid#146994PurposeInsect Expression of DmUPF1-S1140AS1146A-siRNAresDepositorInsertDmUPF1-S1140AS1146A-siRNAres (Upf1 Fly)
UseTagsExpressionInsectMutation1 non silent mutations: G995E compared to the seq…PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmUPF1-S1160A-siRNAres-V5His6_N
Plasmid#146995PurposeInsect Expression of DmUPF1-S1160A-siRNAresDepositorInsertDmUPF1-S1160A-siRNAres (Upf1 Fly)
UseTagsExpressionInsectMutation1 non silent mutations: G995E compared to the seq…PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmUPF1-S1177A-siRNAres-V5His6_N
Plasmid#146996PurposeInsect Expression of DmUPF1-S1177A-siRNAresDepositorInsertDmUPF1-S1177A-siRNAres (Upf1 Fly)
UseTagsExpressionInsectMutation1 non silent mutations: G995E compared to the seq…PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmUPF1-V138ER139E-siRNAres-V5His6_N
Plasmid#146997PurposeInsect Expression of DmUPF1-V138ER139E-siRNAresDepositorInsertDmUPF1-V138ER139E-siRNAres (Upf1 Fly)
UseTagsExpressionInsectMutation1 non silent mutations: G995E compared to the seq…PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only