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Showing: 21 - 40 of 184 results

  2. pSMOX-mNG::LacI-V3

    Plasmid
    #210224
    Purpose
    Integrates a T7 RNA polymerase, tetracycline repressor and an mNeongreen Lac repressor fusion protein with a puromycin drug selection marker into the tubulin locus.
    Depositor
    Calvin Tiengwe
    Article
    Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115
    Insert
    mNeongreen Lac repressor fusion protein
    Use
    Trypanosoma brucei expression
    Tags
    Expression
    Mutation
    Promoter
    Pol II readthrough transcription after integration
    Available since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    210224-plasmid-map-sequence-id-415797

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  4. PP-HSP90 (3O6O)

    Plasmid
    #28153
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    HSP90
    Use
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    N-terminal domain
    Promoter
    Available since
    Feb. 28, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  5. PP-PMM

    Plasmid
    #25202
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    Tb10.70.0370:M1-K245
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids M1-K245.
    Promoter
    Available since
    Aug. 12, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  6. PP-UDPGP

    Plasmid
    #25285
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    Tb10.389.0330:L3-E485
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids L3-E485
    Promoter
    Available since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  7. 6NIM

    Plasmid
    #127294
    Purpose
    Bacterial expression for structure determination. May not contain entire coding region of gene
    Depositor
    Cheryl Arrowsmith
    Insert
    PP-BRD18
    Use
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    127294-plasmid-map-sequence-id-247944

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  8. 5KO4 (PP-BRD19)

    Plasmid
    #101882
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PP-BRD19
    Use
    Tags
    6xHis, TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    101882-plasmid-map-sequence-id-194517

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  9. pTrex-n-eGFP

    Plasmid
    #62544
    Purpose
    Expression of eGFP in T. cruzi. The NheI-linearized form of this plasmid could be efficiently integrated in to T. cruzi genome
    Depositor
    Rick Tarleton
    Article
    Peng et al MBio. 2014 Dec 30;6(1). pii: e02097-14.
    Insert
    eGFP
    Use
    (1)trypanosoma cruzi expression of egfp (2) genom…
    Tags
    None
    Expression
    Mutation
    None
    Promoter
    Ribo-HX1
    Available since
    April 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    62544-plasmid-map-sequence-id-109759

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  10. 5K29 (PP-BRD3)

    Plasmid
    #101883
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PP-BRD3
    Use
    Tags
    6xHis, TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    101883-plasmid-map-sequence-id-194713

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  20. Cas9/pTREX-n

    Plasmid
    #68708
    Purpose
    Expresses the fusion gene CAS9-HA-2xNLS-GFP in the pTREX-n backbone. This vector is used for cloning a specific sgRNA by BamHI, to be co-expressed with Cas9 for genome editing in Trypanosoma cruzi.
    Depositor
    Roberto Docampo
    Article
    Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.
    Insert
    Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 (Addgene) .
    Use
    CRISPR
    Tags
    Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 …
    Expression
    Mutation
    C4239T mutation that eliminates a BamHI restricti…
    Promoter
    Available since
    Sept. 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    68708-plasmid-map-sequence-id-123989

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Showing: 21 - 40 of 184 results