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Showing: 21 - 40 of 6476 results

  8. pCMV-PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetR-FKBP-SbMVseq-SbMVp-SbMVseq-FRB-VP16

    Plasmid
    #210506
    Purpose
    expresses PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetR-FKBP-SbMVseq-SbMVp-SbMVseq-FRB-VP16 component in mammalian cells
    Depositor
    Dongmin Lee
    Insert
    PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetR-FKBP-SbMVseq-SbMVp-SbMVseq-FRB-VP16
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    210506-plasmid-map-sequence-id-415563

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  11. pETDuet_huCAD_ICADL (TevS)

    Plasmid
    #100098
    Purpose
    dual expression of human CAD and ICAD. The two caspase cleavage sites in ICAD were mutated to TEVP cleavable sequences
    Depositor
    Jim Wells
    Article
    Morgan et al Proc Natl Acad Sci U S A. 2015 Jul 7;112
    Insert
    DNA fragmentation factor subunit alpha (CAD, DFFA Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    two caspase cleavage sites (DETD-117 and DAVD-224…
    Promoter
    T7
    Available since
    Jan. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    100098-plasmid-map-sequence-id-261413

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  13. 10xHis-TEV-ATG5_ATG7_ATG10_ATG12_ATG16L1-TEV-StrepII (Human autophagy E3-like enzyme)

    Plasmid
    #169076
    Purpose
    Insect codon optimized ATG genes (excluded tags):10xHis-TEV-ATG5, ATG7, ATG10, ATG12 and ATG16L1-TEV-StrepII
    Depositor
    Sascha Martens
    Article
    Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151
    Inserts
    ATG5 (ATG5 Human)
    ATG7 (ATG7 Human)
    ATG10 (ATG10 Human)
    ATG12 (ATG12 Human)
    ATG16 (ATG16L1 Human)
    Use
    Tags
    GGlinker, TEV cleavage site, StrepII and HIS10 Ta…
    Expression
    Bacterial and Insect
    Mutation
    Promoter
    Polyhedrin promoter
    Available since
    May 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    169076-plasmid-map-sequence-id-416841

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  15. pGBdest-10xHis-TEV-ATG5_ATG7_ATG10_ATG12_ATG16L1_F32A-I35A-I36A_-GFP-TEV-StrepII

    Plasmid
    #192705
    Purpose
    Plasmid for the Expression of the E3 complex with an FII (F32A, I35A, and I36A) mutation. SMC Internal reference: SMC1728
    Depositor
    Sascha Martens
    Inserts
    ATG5 (ATG5 Human)
    ATG7 (ATG7 Human)
    ATG10 (ATG10 Human)
    ATG12 (ATG12 Human)
    ATG16L1
    Use
    Tags
    -GFP-TEV-StrepII and 10x-His-TEV
    Expression
    Insect
    Mutation
    F32A-I35A-I36A
    Promoter
    Available since
    Nov. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    192705-plasmid-map-sequence-id-377544

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  16. pCS2-Prom1-TEVp-TwinStrep-His / IRES2 / Pcdh21-TEVp-Myc-Flag

    Plasmid
    #210820
    Purpose
    Mammalian overexpression vector for polycistronic co-expression of C-terminally Strep and His tagged human Prom1s1 (WT) and C-terminally Myc and Flag tagged human Pchd21 (WT)
    Depositor
    Luke Chao
    Article
    Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7
    Inserts
    Prominin 1 (PROM1 Human)
    Protocadherin 21 (CDHR1 Human)
    Use
    Tags
    Myc, 3xFlag and TwinStrep, 10xHis
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and CMV / IRES2
    Available since
    July 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    210820-plasmid-map-sequence-id-417732

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  18. 10xHis-TEV-ATG5_ATG7_ATG10_ATG12_ATG16L1-TEV-mGFP-StrepII (Human autophagy E3(mGFP)-like enzyme)

    Plasmid
    #169077
    Purpose
    Insect codon optimized ATG genes (excluded tags): 10xHis-TEV-ATG5, ATG7, ATG10, ATG12 and ATG16L1-GFP-TEV-StrepII
    Depositor
    Sascha Martens
    Article
    Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151
    Inserts
    ATG5 (ATG5 Human)
    ATG7 (ATG7 Human)
    ATG10 (ATG10 Human)
    ATG12 (ATG12 Human)
    ATG16 (ATG16L1 Human)
    Use
    Tags
    GFP tag, GGlinker, TEV cleavage site. StrepII and…
    Expression
    Bacterial and Insect
    Mutation
    Promoter
    Polyhedrin promoter
    Available since
    May 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169077-plasmid-map-sequence-id-332050

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  19. pFastBacMam7_N_Flag_TEV

    Plasmid
    #178817
    Purpose
    Baculovirus gene transfer into Mammalian cells to product recombinant proteins.
    Depositor
    Kazuhiro Abe
    Type
    Empty backbone
    Use
    Tags
    Flag, TEV
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    178817-plasmid-map-sequence-id-351922

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Showing: 21 - 40 of 6476 results