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EGFP-SPN_ARR wt (Shank3)

Plasmid

#175254

Purpose

SPN-ARR fragment in EGFP backbone for mammalian expression

Depositor

Johanna Ivaska

Article

Salomaa et al Curr Biol. 2021 Sep 29. pii: S0960-9822(

Insert

SPN-ARR fragment from SHANK3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pET28b(+)-SPN+IFS

Plasmid

#83372

Purpose

Expresses SPN and IFS complex in E. coli cell

Depositor

SeWon Suh

Article

Yoon et al J Synchrotron Radiat. 2013 Nov;20(Pt 6):

Inserts

SPN
IFS

Use

Tags

Hexahistidine tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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PX458-SPNS1-sg1

Plasmid

#198566

Purpose

SPNS1-targeting sgRNA in PX458 vector

Depositor

Monther Abu-Remaileh

Article

Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

Insert

SPNS1-sg1 (SPNS1 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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PX458-SPNS1-sg2

Plasmid

#198567

Purpose

SPNS1-targeting sgRNA in PX458 vector

Depositor

Monther Abu-Remaileh

Article

Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

Insert

SPNS1-sg2 (SPNS1 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pMXs-3xFlag-SPNS1

Plasmid

#198568

Purpose

SPNS1 codon-optimized orf with n-terminal 3xFlag in pMXs-IRES-Blast vector

Depositor

Monther Abu-Remaileh

Article

Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

Insert

SPNS1 with n-terminal 3xFlag (SPNS1 Human)

Use

Retroviral

Tags

3xFlag

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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plentiCRISPR-v2 Puro SPNS1_sg2

Plasmid

#218531

Purpose

sgRNA targeting human SPNS1

Depositor

Kivanc Birsoy

Article

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

SPNS1 (SPNS1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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plentiCRISPR-v2 Puro SPNS1_sg1

Plasmid

#218530

Purpose

sgRNA targeting human SPNS1

Depositor

Kivanc Birsoy

Article

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

SPNS1 (SPNS1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsPNRC2_V

Plasmid

#147760

Purpose

Mammalian Expression of HsPNRC2

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsPNRC2 (PNRC2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pFHMSPN-avi-TEV-LIC V

Plasmid

#220885

Purpose

Donor vector to generate recombinant baculovirus by site-specific transposition in an E. coli host.

Depositor

Cheryl Arrowsmith

Type

Empty backbone

Use

Baculovirus expresssion

Tags

Honeybee melittin signal followed by His, avi and…

Expression

Mutation

Promoter

Available since

June 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pCIneo-V5-SBP-MBP-HsPNRC2_V

Plasmid

#147761

Purpose

Mammalian Expression of HsPNRC2

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsPNRC2 (PNRC2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-SPN_ARR N52R (Shank3)

Plasmid

#175255

Purpose

SPN-ARR fragment with structure opening N52R mutation in EGFP backbone for mammalian expression

Depositor

Johanna Ivaska

Article

Salomaa et al Curr Biol. 2021 Sep 29. pii: S0960-9822(

Insert

SPN-ARR fragment with N52R mutation from SHANK3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-IRES-blast GFP-SPNS1

Plasmid

#198571

Purpose

SPNS1 codon-optimized orf with n-terminal 3xFlag GFP in pMXs-IRES-Blast vector

Depositor

Monther Abu-Remaileh

Article

Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

Insert

SPNS1 with n-terminal 3xFlag-GFP fusion (SPNS1 Human)

Use

Retroviral

Tags

3xFlag-GFP

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pMXs-IRES-blast 3xFlag-SPNS1(E164K)

Plasmid

#198569

Purpose

SPNS1(E164K) codon-optimized orf with n-terminal 3xFlag in pMXs-IRES-Blast vector

Depositor

Monther Abu-Remaileh

Article

Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

Insert

SPNS1(E164K) with n-terminal 3xFlag (SPNS1 Human)

Use

Retroviral

Tags

3xFlag

Expression

Mammalian

Mutation

changed glutamic acid E164 to lysine (E164K)

Promoter

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pMXs-IRES-blast 3xFlag-SPNS1(R76A)

Plasmid

#198570

Purpose

SPNS1(R76A) codon-optimized orf with n-terminal 3xFlag in pMXs-IRES-Blast vector

Depositor

Monther Abu-Remaileh

Article

Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

Insert

SPNS1(R76A) with n-terminal 3xFlag (SPNS1 Human)

Use

Retroviral

Tags

3xFlag

Expression

Mammalian

Mutation

changed arginine R76 to alanine (R76A)

Promoter

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pMXs-IRES-blast GFP-SPNS1(E164K)

Plasmid

#198572

Purpose

SPNS1(E164K) codon-optimized orf with n-terminal 3xFlag GFP in pMXs-IRES-Blast vector

Depositor

Monther Abu-Remaileh

Article

Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

Insert

SPNS1(E164K) with n-terminal 3xFlag-GFP fusion (SPNS1 Human)

Use

Retroviral

Tags

3xFlag-GFP

Expression

Mammalian

Mutation

changed glutamic acid E164 to lysine (E164K)

Promoter

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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EGFP-SPN_ARR Q37A/R38A/N52R (Shank3)

Plasmid

#175256

Purpose

SPN-ARR fragment with structure opening N52R and actin binding site Q37A/R38A mutations in EGFP backbone for mammalian expression

Depositor

Johanna Ivaska

Article

Salomaa et al Curr Biol. 2021 Sep 29. pii: S0960-9822(

Insert

SPN-ARR fragment with Q37A/R38A/N52R mutations from SHANK3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-MYL2-TadA8e-SpN aa2-713- inteinN-U6-Camk2d sgRNA7

Plasmid

#226915

Purpose

Expresses TadA8e and Sp cas9N by MYL2 promoter and sgRNA targeting murine Camk2d intron7 5' splice site by U6 promoter

Depositor

Yuxuan Guo

Article

Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

Insert

MYL2, TadA, nSp Cas9N, inteinN

Use

AAV

Tags

Expression

Mutation

Promoter

MYL2

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCM5.50

Plasmid

#19383

Depositor

Geraldine Seydoux

Article

Merritt et al Curr Biol. 2008 Sep 23. ():.

Insert

spn-4 3'utr in pDONRP2RP3 (spn-4 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only

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pCM1.120

Plasmid

#19600

Depositor

Geraldine Seydoux

Insert

spn-4 prom in PDONRP4P1R

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only

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pDR3.01

Plasmid

#19369

Depositor

Geraldine Seydoux

Article

Merritt et al Curr Biol. 2008 Sep 23. ():.

Insert

spn-4 ORF+3' in pDONR201

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Jan. 21, 2009

Availability

Academic Institutions and Nonprofits only

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