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pAtU6-sgRNA

Plasmid

#119775

Purpose

Expression of a target sgRNA in potato

Depositor

Caixia Gao

Article

Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

Insert

AtU6-SmR-sgRNA

Use

Tags

Expression

Plant

Mutation

Promoter

AtU6

Available since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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sgRNA AAVS1

Plasmid

#128119

Purpose

Co-expresses sgRNA AAVS1, SpyCas9 scaffold (F+E) and GFP (transfection marker)

Depositor

Dominik Niopek

Article

Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

Insert

sgRNA AAVS1 + GFP

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

RSV/U6

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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sgRNA EMX1

Plasmid

#128121

Purpose

Co-expresses sgRNA EMX1, SpyCas9 scaffold (F+E) and GFP (transfection marker)

Depositor

Dominik Niopek

Article

Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

Insert

sgRNA EMX1 + GFP

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

RSV/U6

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pCF142_U6-sgRNA

Plasmid

#225960

Purpose

U6-sgRNA (recipient). Expresses U6-sgRNA (recipient) for VLP production. This is a recipient vector for cloning of specific SpyCas9 sgRNAs.

Depositor

Christof Fellmann

Article

Ramadoss et al bioRxiv [Preprint]. 2024 Jun 26:2024.06.

Insert

U6-sgRNA (recipient)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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ATG13 sgRNA

Plasmid

#207558

Purpose

pX330 expressing Cas9 and a sgRNA targeting the ATG13 locus

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

ggaaactgatctcaattccc

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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ATG9A sgRNA

Plasmid

#207557

Purpose

pX330 expressing Cas9 and a sgRNA targeting the ATG9A locus

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

CACTGAATACCAGCGCCTAG

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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WIPI2 sgRNA

Plasmid

#207554

Purpose

pX330 expressing Cas9 and a sgRNA targeting the WIPI2 locus

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

CGCGCGCCCAGCCATGAACC

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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sgRNA HBB

Plasmid

#128120

Purpose

Co-expresses sgRNA HBB, SpyCas9 scaffold (F+E) and GFP (transfection marker)

Depositor

Dominik Niopek

Article

Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

Insert

sgRNA HBB + GFP

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

RSV/U6

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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sgRNA RUNX

Plasmid

#128122

Purpose

Co-expresses sgRNA RUNX, SpyCas9 scaffold (F+E) and GFP (transfection marker)

Depositor

Dominik Niopek

Article

Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

Insert

sgRNA RUNX + GFP

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

RSV/U6

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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sgRNA HEK

Plasmid

#128123

Purpose

Co-expresses sgRNA HEK, SpyCas9 scaffold (F+E) and GFP (transfection marker)

Depositor

Dominik Niopek

Article

Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

Insert

sgRNA HEK + GFP

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

RSV/U6

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pTBL509 AT sgRNA

Plasmid

#126429

Purpose

To target a protospacer with AT at the cut site.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

spacer against human genome with AT at cut site

Use

Tags

Expression

Mammalian

Mutation

Promoter

human U6

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAGM4723::AtU6p::sgRNA2-2x35S-5′UTR::Cas9::NOST-AtU6p::sgRNA1

Plasmid

#49772

Purpose

Level 2 construct

Depositor

Sophien Kamoun

Article

Belhaj et al Plant Methods. 2013 Oct 11;9(1):39.

Insert

sgRNA_PDS2-Cas9-sgRNA_PDS1

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Sept. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pBXMCS-2 Pconstitutive-sgRNA(Sth3)_cpaA- Pconstitutive-sgRNA(Sth3)_blaA

Plasmid

#133347

Purpose

expression of two sgRNA from Streptococcus thermophilus #3 each express from its own constitutive promoter; here first one targets cpaA and second one targets blaA (from Caulobacter crescentus)

Depositor

Michael Laub

Article

Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

Insert

sgRNA_cpaA and sgRNA_blaA

Use

CRISPR

Tags

Expression

Mutation

Promoter

constitutive

Available since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pBXMCS-2 Pconstitutive-sgRNA(Sth3)_blaA- Pconstitutive-sgRNA(Sth3)_cpaA

Plasmid

#133348

Purpose

expression of two sgRNA from Streptococcus thermophilus #3 each express from its own constitutive promoter; here first one targets blaA and second one targets cpaA (from Caulobacter crescentus)

Depositor

Michael Laub

Article

Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

Insert

sgRNA_blaA and sgRNA_cpaA

Use

CRISPR

Tags

Expression

Mutation

Promoter

constitutive

Available since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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LCV2_LacZ_sgRNA_2

Plasmid

#155093

Purpose

lentiviral plasmid expressing Cas9 and gRNA targeting LacZ

Depositor

Jason Moffat

Article

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Insert

LacZ_sgRNA_2

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6 promoter

Available since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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PtPuc3_diaCas9_sgRNA

Plasmid

#109219

Purpose

Episome based vector with CRISPR/Cas9_sgRNA module for genome editing in Phaeodactylum tricornutum

Depositor

Per Winge

Article

Sharma et al Sci Rep. 2018 Sep 26;8(1):14401. doi: 10

Inserts

LHCF2 promoter
diaCas9
LHCF1 terminator
U6 promoter
sgRNA
U6 3' region

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

LHCF1 terminator, LHCF2 promoter, U6 3' regi…

Available since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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psgRNA

Plasmid

#114005

Purpose

express sgRNA. ColE1, Kan

Depositor

David Bikard

Article

Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

Insert

gRNA

Use

Tags

Expression

Mutation

Promoter

BBa_J23119

Available since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pU6b:sgRNA#3

Plasmid

#64247

Purpose

expresses sgRNA under U6b promoter

Depositor

Wenbiao Chen

Article

Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

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pU6a:sgRNA#1

Plasmid

#64245

Purpose

expresses sgRNA under U6a promoter

Depositor

Wenbiao Chen

Article

Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

May 7, 2015

Availability

Academic Institutions and Nonprofits only

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pU6c:sgRNA#4

Plasmid

#64248

Purpose

expresses sgRNA under U6c promoter

Depositor

Wenbiao Chen

Article

Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge