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Plasmid#139971PurposeFusion of A. viridans lactate oxidase (LOX) and E. coli catalase (CAT) that converts lactate into pyruvate without producing H2O2. Contains a albumin binding peptide (ABP).DepositorInsertA fusion of lactate oxidase from A. viridans and catalase from E. coli
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET30a-SARS-CoV2-NP-Nt(47-173)
Plasmid#165091Purposeprotein expression in E. coliDepositorInsertSevere acute respiratory syndrome coronavirus 2 nucleocapsid protein N-terminal(47-173) (N SARS-CoV2)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 22, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET30a-His6-ABP-LOXCATmut
Plasmid#139972PurposeA control for His6-ABP-LOXCAT where LOX is catalytically dead (mutations H265A and R268A)DepositorInsertA fusion of lactate oxidase from A. viridans and catalase from E. coli
UseTagsHis6ExpressionBacterialMutationMutation in H265A and R268A in LOX (used original…PromoterT7Available sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET30a-SARS-CoV2-ORF8
Plasmid#165089Purposeprotein expression in E. coliDepositorInsertSevere acute respiratory syndrome coronavirus 2 ORF8 (ORF8 SARS-CoV2)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 10, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET30a-SARS-CoV2-ORF3b (S23Q)
Plasmid#165088Purposeprotein expression in E. coliDepositorInsertSevere acute respiratory syndrome coronavirus 2 ORF3b (S23Q)
UseTagsExpressionBacterialMutationStop codon to glutamine at 23rd amino acidPromoterAvailable sinceFeb. 10, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET30a-ASXL1-HARE-HTH domain
Plasmid#107164PurposeExpresses murine ASXL1 HARE-HTH domain (amino acids 2-83) in E. coliDepositorInsertASXL1 (Asxl1 Mouse)
UseTagsFlagExpressionBacterialMutationContains amino acids 2-83PromoterT7Available sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET30a- phage MazG-like/Atd1
Plasmid#195244PurposeExpersses phage MazG-like/Atd1DepositorInsertphage MazG
UseTagsFlag and TwinStrepExpressionBacterialMutationCatalytic site mutantPromoterT7Available sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
hFerritinL
Plasmid#122653PurposeExpression of hFerritinL in bacterial cellsDepositorInsertlight chain ferritin, human (FTL Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
hFerritinH
Plasmid#122652PurposeExpression of hFerritinH in bacterial cellsDepositorInsertferritin heavy chain 1 (FTH1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET30-GBFusion1
Plasmid#133438PurposeStreptococcus GB1 domain in vector for bacterial expression.DepositorInsertGB1 domain
UseTagsExpressionBacterialMutationThis fragment encodes only aa 1 to 56.PromoterAvailable sinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
LisDPS
Plasmid#122654PurposeExpression of LisDPS in bacterial cellsDepositorInsertDPS, listeria
UseTagsExpressionBacterialMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pET30-T7-sfGFP
Plasmid#180757PurposepET30 vector missing lacO and N-terminal His tag with sfGFP-S-tag inserted between the Bgl II and T7 terminatorDepositorInsertsuperfolder green fluorescent protein
UseTagsstrep tagExpressionBacterialMutationPromoterAvailable sinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
His-PaIct
Plasmid#111293Purposebacterial expressionDepositorInsertsuccinyl-CoA:itaconate CoA transferase
UseTagsHis tagExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET30-T7-His-sfGFP
Plasmid#180755PurposepET30 vector missing lacO with sfGFP-S-tag inserted between the Bgl II and T7 terminatorDepositorInsertsuperfolder green fluorescent protein
UseTagsstrep tagExpressionBacterialMutationPromoterAvailable sinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET30-T7-lacO-sfGFP
Plasmid#180756PurposepET30 vector missing N-terminal His tag with sfGFP-S-tag inserted between the Bgl II and T7 terminatorDepositorInsertsuperfolder green fluorescent protein
UseTagsstrep tagExpressionBacterialMutationPromoterAvailable sinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET30 NHERF1
Plasmid#32854DepositorInsertsolute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1 (SLC9A3R1 Rabbit)
UseTagsS16 and hisExpressionBacterialMutationnonePromoterT7Available sinceFeb. 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET30-T7-lacO-His-sfGFP
Plasmid#180754PurposepET30 vector with sfGFP-S-tag inserted between the Bgl II and T7 terminatorDepositorInsertsuperfolder green fluorescent protein
UseTagsstrep tagExpressionBacterialMutationG140C- Please see depositor commentsPromoterAvailable sinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
mP14H1
Plasmid#84369Purposeantigen for mouse PRDM14 purification ( aa 1-231)DepositorInsertPRDM14
UseTags6xHisExpressionBacterialMutationonly aa 1-231 presentPromoterT7Available sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pM9-BetaGal-8C
Plasmid#172489PurposePlasmid encoding E. coli BetaGalactosidase with an N-terminal M9 nuclear localization sequenceDepositorInsertM9-BetaGal-8C
UseTags6xHis and M9 nuclear localization sequence (from …ExpressionBacterialMutationBetaGal has two cysteine mutations (C77A, C1022S)…PromoterT7Available sinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only