Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
Showing: 21 - 40 of 58 results

  1. qTAG-N-Puro-moxGFP-GOLGA2

    Plasmid
    #207792
    Purpose
    Donor template for Puro-2A-moxGFP insertion into the N-terminus of the GOLGA2 locus for Golgi visualization. To be co-transfected with sgRNA plasmid px330-PITCh-GOLGA2 Addgene #207791
    Depositor
    Laurence Pelletier
    Article
    Philip et al bioRxiv
    Insert
    GOLGA2 Homology Arms flanking a Puro-moxGFP Cassette (GOLGA2 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    207792-plasmid-map-sequence-id-413795

    Enlarge

  2. qTAG-C-moxGFP-Puro-MAPRE1

    Plasmid
    #207794
    Purpose
    Donor template to insert moxGFP-2A-Puro into the C-terminus of the MAPRE1 locus for growing microtubule tip visualization. To be co-transfected with sgRNA plasmid px330-PITCh-MAPRE1 Addgene #207793
    Depositor
    Laurence Pelletier
    Article
    Philip et al bioRxiv
    Insert
    MAPRE1 Homology Arms flanking a moxGFP-Puro Cassette (MAPRE1 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    207794-plasmid-map-sequence-id-416380

    Enlarge

  4. qTAG-C-moxGFP-Puro-H2BC11-MMEJ

    Plasmid
    #207760
    Purpose
    MMEJ donor template for moxGFP-2A-Puro insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755
    Depositor
    Laurence Pelletier
    Article
    Philip et al bioRxiv
    Insert
    H2BC11 Short Homology Arms flanking a moxGFP-Puro Cassette (H2BC11 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    207760-plasmid-map-sequence-id-413789

    Enlarge

  8. ST*-GFP

    Plasmid
    #163667
    Purpose
    Expresses partial length ST in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Insert
    partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 (first 113 amino acids) (ST6GAL1 Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    This chimera contains the first 113 amino acids o…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    163667-plasmid-map-sequence-id-322800

    Enlarge

  9. F9F17F-GFP

    Plasmid
    #163679
    Purpose
    Expresses a TFR mutant with truncated cytosolic tail and TMD in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Insert
    transferrin receptor mutant (TFRC Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    This chimera contains 9 amino acids from the cyto…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    163679-plasmid-map-sequence-id-322819

    Enlarge

  11. SBP-ST*-GFP

    Plasmid
    #163668
    Purpose
    Expresses SBP tagged partial length ST in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Insert
    partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 (first 113 amino acids) (ST6GAL1 Human)
    Use
    Tags
    GFP and Streptavidin Binding Protein (SBP)
    Expression
    Mammalian
    Mutation
    This chimera contains the first 113 amino acids o…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    163668-plasmid-map-sequence-id-322801

    Enlarge

  12. FKBP-ST*-GFP

    Plasmid
    #163669
    Purpose
    Expresses FKBP tagged partial length ST in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Insert
    partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 (first 113 amino acids) (ST6GAL1 Human)
    Use
    Tags
    FK506 binding protein (FKBP) and GFP
    Expression
    Mammalian
    Mutation
    This chimera contains the first 113 amino acids o…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    163669-plasmid-map-sequence-id-322815

    Enlarge

  13. TMD18

    Plasmid
    #163673
    Purpose
    Expresses a ST6GAL1 mutant with 18 amino acids in its TMD in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Insert
    partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    For this partial length ST chimera, two restricti…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    163673-plasmid-map-sequence-id-322677

    Enlarge

  14. TMD19

    Plasmid
    #163674
    Purpose
    Expresses a ST6GAL1 mutant with 19 amino acids in its TMD in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Insert
    partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    For this partial length ST chimera, two restricti…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    163674-plasmid-map-sequence-id-322678

    Enlarge

  15. FSS-GFP

    Plasmid
    #163654
    Purpose
    Expresses a TFR and ST6GAL1 swapped chimera with the cytosolic tail from TFR and TMD and luminal domain from ST6GAL1 in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Insert
    ST6 beta-galactoside alpha-2,6-sialyltransferase 1 and transferrin receptor (ST6GAL1, TFRC Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    This chimera contains the cytosolic tail from TFR…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    163654-plasmid-map-sequence-id-322771

    Enlarge

  16. SSF-GFP

    Plasmid
    #163656
    Purpose
    Expresses a TFR and ST6GAL1 swapped chimera with the cytosolic tail and TMD from ST6GAL1, and luminal domain from TFR in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Insert
    ST6 beta-galactoside alpha-2,6-sialyltransferase 1 and transferrin receptor (ST6GAL1, TFRC Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    This chimera contains the cytosolic tail from ST,…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    163656-plasmid-map-sequence-id-322782

    Enlarge

  17. SFF-GFP

    Plasmid
    #163657
    Purpose
    Expresses a TFR and ST6GAL1 swapped chimera with the cytosolic tail from ST6GAL1, and TMD and luminal domain from TFR in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Insert
    ST6 beta-galactoside alpha-2,6-sialyltransferase 1 and transferrin receptor (ST6GAL1, TFRC Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    This chimera contains the cytosolic tail from ST,…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    163657-plasmid-map-sequence-id-322784

    Enlarge

  18. FFS-GFP

    Plasmid
    #163659
    Purpose
    Expresses a TFR and ST6GAL1 swapped chimera with the cytosolic tail and TMD from TFR, and luminal domain from ST6GAL1 in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Insert
    ST6 beta-galactoside alpha-2,6-sialyltransferase 1 and transferrin receptor (ST6GAL1, TFRC Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    This chimera contains the cytosolic tail from TFR…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    163659-plasmid-map-sequence-id-322790

    Enlarge

  19. NSS-GFP

    Plasmid
    #163661
    Purpose
    Expresses a TNF and ST6GAL1 swapped chimera with the cytosolic tail from TNF and TMD and luminal domain from ST6GAL1 in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Insert
    ST6 beta-galactoside alpha-2,6-sialyltransferase 1 and tumor necrosis factor (ST6GAL1, TNF Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    This chimera contains the cytosolic tail from TNF…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    163661-plasmid-map-sequence-id-322793

    Enlarge

  20. SSN-GFP

    Plasmid
    #163663
    Purpose
    Expresses a TNF and ST6GAL1 swapped chimera with the cytosolic tail and TMD from ST6GAL1, and luminal domain from TNF in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Insert
    ST6 beta-galactoside alpha-2,6-sialyltransferase 1 and tumor necrosis factor (ST6GAL1, TNF Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    This chimera contains the cytosolic tail from ST,…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    163663-plasmid-map-sequence-id-322796

    Enlarge

Showing: 21 - 40 of 58 results