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pGSTag BimEL

Plasmid

#23103

Depositor

Roger Davis

Article

Hubner et al Mol Cell. 2008 May 23. 30(4):415-25.

Insert

pGSTag Bim EL (Bcl2l11 Mouse)

Use

Tags

GSTag

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 24, 2010

Availability

Academic Institutions and Nonprofits only
pIS1 mELOVL6

Plasmid

#60791

Purpose

Luciferase reporter to measure miR-155 mediated differential repression. Contains ELOVL6 3' UTR and mutated miR-155 sites

Depositor

David Bartel

Article

Nam et al Mol Cell. 2014 Mar 20;53(6):1031-43. doi

Insert

ELOVL6 3'UTR and mutated miR-155 binding site (ELOVL6 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

mutated miR-155 binding site

Promoter

thymidine kinase (HSV-TK promoter)

Available since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pHR_CMV_MeltEGFR-37-mCherry

Plasmid

#234065

Purpose

Encodes EGFR intracellular domain controlled by a Melt variant with a switching temperature of 37°C

Depositor

Lukasz Bugaj

Article

Benman et al Nat Methods. 2025 Jan 23. doi: 10.1038/s

Insert

MeltEGFR-37-mCherry

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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pHR_CMV_MeltTEVp-40-mCherry

Plasmid

#234064

Purpose

Encodes a TEV protease controlled by a Melt variant with a switching temperature of 40°C

Depositor

Lukasz Bugaj

Article

Benman et al Nat Methods. 2025 Jan 23. doi: 10.1038/s

Insert

MeltTEV-40-mCherry

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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pHR_CMV_Melt-30-mCherry

Plasmid

#234061

Purpose

Encodes a Melt variant with a switching temperature of 30°C

Depositor

Lukasz Bugaj

Article

Benman et al Nat Methods. 2025 Jan 23. doi: 10.1038/s

Insert

Melt-30-mCherry

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pHR_CMV_Melt-32-mCherry

Plasmid

#234062

Purpose

Encodes a Melt variant with a switching temperature of 32°C

Depositor

Lukasz Bugaj

Article

Benman et al Nat Methods. 2025 Jan 23. doi: 10.1038/s

Insert

Melt-32-mCherry

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pHR_CMV_MeltCasp1-37-GFP

Plasmid

#234069

Purpose

Encodes the caspase1 without CARD domain (with R391K mutation) controlled by a Melt variant with a switching temperature of 37°C

Depositor

Lukasz Bugaj

Article

Benman et al Nat Methods. 2025 Jan 23. doi: 10.1038/s

Insert

MeltCasp1-37-GFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Melt(C292A,Q355N); hcaspase1(R391K)

Promoter

CMV

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pET28b-MelG

Plasmid

#225729

Purpose

To express MelG (Rv1937) in E. coli

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

melG

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pIRES2_hMela(CT mGluR6)

Plasmid

#191344

Purpose

Optogenetic tool

Depositor

Sonja Kleinlogel

Article

Kralik et al Commun Biol. 2022 Oct 20;5(1):1116. doi:

Insert

hMela(CTmGluR6)

Use

Tags

IRES_Turbo

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pET28b-MelI

Plasmid

#225730

Purpose

To express MelI (Rv1939) in E. coli

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

melI

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pET28b-MelK

Plasmid

#225732

Purpose

To express MelK (Rv1941) in E. coli

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

melK

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pET28b-MelJ

Plasmid

#225731

Purpose

To express MelJ (Rv1940) in E. coli

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

melJ

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pLV_Tre3g_CalcireticulinSS_mTurquoise_D4-cameleon_REAch2_KDEL

Plasmid

#216253

Purpose

FRET-based calcium sensor located in the ER lumen, Tet-inducible

Depositor

Edward Avezov

Article

Crapart et al Proc Natl Acad Sci U S A. 2024 Mar 26;12

Insert

ER-targeted D4 second generation cameleon (FRET calcium sensor)

Use

Lentiviral

Tags

REAch and mTurquoise

Expression

Mutation

Promoter

TRE3G

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA4-Flag-Timeless

Plasmid

#22887

Depositor

Aziz Sancar

Article

Unsal-Kacmaz et al Mol Cell Biol. 2005 Apr . 25(8):3109-16.

Insert

Timeless (TIMELESS Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

deleted normal start codon, replaced with start c…

Promoter

Available since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only

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pEGFP-hTimeless (pc4729)

Plasmid

#216861

Purpose

Mammalian expression vector encoding GFP tagged human Timeless

Depositor

Cristina Cardoso

Article

Prorok et al Front Cell Dev Biol. 2024 Feb 29;12:1346

Insert

Timeless (TIMELESS Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 11, 2024

Availability

Academic Institutions and Nonprofits only

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pHR_CMV_MeltNLS/NES-40-mCherry

Plasmid

#234066

Purpose

Encodes a protein that localizes to nucleus under the control of a Melt variant with a switching temperature of 40°C

Depositor

Lukasz Bugaj

Article

Benman et al Nat Methods. 2025 Jan 23. doi: 10.1038/s

Insert

MeltNLS/NES-40-mCherry

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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pDmelOR-mRho.V5.mER.hOr56a

Plasmid

#126479

Purpose

Human codon-optimized D. melanogaster Orco (hOrco) and Or56a (hOr56a). Or56a has N-terminal tags derived from human Rhodopsin (mRho) and HCN1 (mER) for improved trafficking in mammalian cells

Depositor

Bill Hansson

Article

Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.

Inserts

Or56a (Or56a Fly)
Orco (Orco Fly)

Use

Tags

H. sapiens HCN1 106VNKFSL111 (mER), H. sapiens Rh…

Expression

Mammalian

Mutation

codon optimization for H. sapiens and codon optim…

Promoter

CMV

Available since

June 13, 2019

Availability

Academic Institutions and Nonprofits only

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pDmelOR-mRho.V5.mER.hOr47a

Plasmid

#126478

Purpose

Human codon-optimized D. melanogaster Orco (hOrco) and Or47a (hOr47a). Or47a has N-terminal tags derived from human Rhodopsin (mRho) and HCN1 (mER) for improved trafficking in mammalian cells

Depositor

Bill Hansson

Article

Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.

Inserts

Or47a (Or47a Fly)
Orco (Orco Fly)

Use

Tags

H. sapiens HCN1 106VNKFSL111 (mER), H. sapiens Rh…

Expression

Mammalian

Mutation

codon optimization for H. sapiens and codon optim…

Promoter

CMV

Available since

June 13, 2019

Availability

Academic Institutions and Nonprofits only

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pFastBac1-Flag-Timeless

Plasmid

#23119

Depositor

Aziz Sancar

Article

Kemp et al J Biol Chem. 2010 Mar 15. ():.

Insert

Timeless (TIMELESS Human)

Use

Tags

Flag

Expression

Insect

Mutation

Promoter

Available since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)_Pmel-1_TCRB

Plasmid

#175288

Purpose

Expresses Pmel-1 TCR beta chain

Depositor

Jeff Hammerbacher

Article

Gottschalk et al bioRxiv 2021.11.30.470597

Insert

T cell receptor beta chain (Tcrb Mouse)

Use

Suitable for ivt

Tags

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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