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Plasmid#224436PurposeConstruct for labelling cell nuclei in mammalian cells using Histon protein 2B fused to mScarlet3DepositorInsertH2B-mScarlet3
UseTagsExpressionMammalianMutationPromoterCMV & SP6Available sinceSept. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSEED-mScarlet3:ALFA
Plasmid#219641PurposePermits GoldenGate assembly (BsmBI) of donor plasmids to knock-in mScarlet3:ALFA using the SEED/Harvest methodDepositorInsertmScarlet:ALFA SEED Cassette
UseTagsExpressionMutationPromoterAvailable sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDRESS_mTurquoise2_spatial-linker-P2A_mScarlet3
Plasmid#189752PurposeProduces an mTurquoise-antiFRET-mScarlet3 fusion protein in bacteria and in mamallian cells it produces 1:1 unfused mTurquoise2 and mScarlet3 FPs for ratiometric analysisDepositorInsertmTurquoise2_spatial-linker-P2A_mScarlet3
UseTags6xHisExpressionBacterial and MammalianMutationPromoterCMV & RhaAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
3_T7-mScarlet3-KRas6R
Plasmid#225930PurposeIn vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the K-Ras6R membrane localisation signalDepositorInsertmScarlet3-KRas6R
UseIn vitro transcriptionTagsExpressionMutationPromoterT7Available sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
10_T7-SP-CD8tm-mScarlet3
Plasmid#225937PurposeIn vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with a signal peptide and the CD8 transmembrane domainDepositorInsertSP-CD8tm-mScarlet3
UseIn vitro transcriptionTagsExpressionMutationPromoterT7Available sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDx_mScarlet3-NCwt-SYFP2
Plasmid#189766PurposeDual expression vector for bacteria and mammalian cells producing mScarlet3-NCwt-SYFP2 fusion protein for FRET analysisDepositorInsertmScarlet3-NCwt-SYFP2
UseTags6xHisExpressionBacterial and MammalianMutationencodes the expected internal mutations relative …PromoterCMV & RhaAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
1_T7-mScarlet3-Hras
Plasmid#225928PurposeIn vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the H-Ras membrane localisation signalDepositorInsertmScarlet3-HRas
UseIn vitro transcriptionTagsExpressionMutationPromoterT7Available sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
2_T7-mScarlet3-KRas
Plasmid#225929PurposeIn vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the K-Ras membrane localisation signalDepositorInsertmScarlet3-KRas
UseIn vitro transcriptionTagsExpressionMutationPromoterT7Available sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
4_T7-mScarlet3-RitC
Plasmid#225931PurposeIn vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the H-RitC membrane localisation signalDepositorInsertmScarlet3-RitC
UseIn vitro transcriptionTagsExpressionMutationPromoterT7Available sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
5_T7-GAP43-mScarlet3
Plasmid#225932PurposeIn vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the GAP43 membrane localisation signalDepositorInsertGAP43-mScarlet3
UseIn vitro transcriptionTagsExpressionMutationPromoterT7Available sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
6_T7-Lyn-mScarlet3
Plasmid#225933PurposeIn vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the Lyn membrane localisation signalDepositorInsertLyn-mScarlet3
UseIn vitro transcriptionTagsExpressionMutationPromoterT7Available sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
7_T7-Src64B-mScarlet3
Plasmid#225934PurposeIn vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the Src64B membrane localisation signalDepositorInsertSrc64B-mScarlet3
UseIn vitro transcriptionTagsExpressionMutationPromoterT7Available sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
9_T7-mScarlet3-LactC2
Plasmid#225936PurposeIn vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the LactC2 membrane localisation signalDepositorInsertmScarlet3-LactC2
UseIn vitro transcriptionTagsExpressionMutationPromoterT7Available sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDRF1 mScarlet3-T2A-mTurquoise2
Plasmid#219848Purposeequimolar expression of mScarlet3 and mTurquoise2 in budding yeastDepositorInsertmScarlet3-T2A-mTurquoise2
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 27, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCS2+mScarlet3-C Cloning Vector
Plasmid#220616PurposeTo express a protein of interest fused to the C-terminus of mScarlet3 (unpublished)DepositorTypeEmpty backboneUseTagsmScarlet3 fusionExpressionMammalianMutationPromoterCMVAvailable sinceJune 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-mScarlet3-CAAX-IRES-Puro
Plasmid#222639PurposeLive-cell plasma membrane markerDepositorInsertmScarlet3-CAAX
UseLentiviralTagsmScarlet3ExpressionMutationPromoterEF1aAvailable sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Cyto-iPASnFR-L-mScarlet3-3xFLAG
Plasmid#225242PurposeMammalian expression of iPASnFR-L in the cytosolDepositorInsertiPASnFR-L
UseTags3xFLAG and mScarlet3ExpressionMammalianMutationPromoterAvailable sinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Cyto-iPASnFR-H-mScarlet3-3xFLAG
Plasmid#225241PurposeMammalian expression of iPASnFR-H in the cytosolDepositorInsertiPASnFR-H
UseTags3xFLAG and mScarlet3ExpressionMammalianMutationPromoterAvailable sinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-NLS-iPASnFR-L-mScarlet3-3xFLAG
Plasmid#225246PurposeMammalian expression of iPASnFR-L in the nucleusDepositorInsertiPASnFR-L
UseTags3xFLAG and mScarlet3ExpressionMammalianMutationPromoterAvailable sinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-cox8-iPASnFR-L-mScarlet3-3xFLAG
Plasmid#225240PurposeMammalian expression of iPASnFR-L in mitochondriaDepositorInsertiPASnFR-L
UseTags3xFLAG and mScarlet3ExpressionMammalianMutationPromoterAvailable sinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only