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pMax_mOrange_A215C

Plasmid

#177828

Purpose

Site-specific mutagenesis of mOrange for the purposes of creating a site-specific 8-oxo-G:C lesion for the purposes of measuring OGG1 activity via Host Cell Reactivation (HCR).

Depositor

Leona Samson, Zachary Nagel

Article

Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

Insert

mOrange_A215C

Use

Tags

Expression

Mammalian

Mutation

A215C

Promoter

CMV

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pPSmOrange2-N1

Plasmid

#34958

Depositor

Vladislav Verkhusha

Article

Subach et al J Am Chem Soc. 2012 Aug 17.

Insert

PSmOrange2

Use

Tags

Expression

Mammalian

Mutation

R17H, R36H, F64I, Q194L, A224S, G226A (mutations …

Promoter

Available since

Aug. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pKB01_mOrange2

Plasmid

#69102

Purpose

Expresses mOrange2 (codon adapted for Streptococcus pneumoniae); under control of Zinc inducible promoter PZn. Plasmid integrates in S. pneumoniae bgaA locus by double cross-over.

Depositor

Jan-Willem Veening

Article

Beilharz et al Appl Environ Microbiol. 2015 Aug 7. pii:

Insert

mOrange2

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Oct. 2, 2015

Availability

Academic Institutions and Nonprofits only

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mOrange2-H3-23

Plasmid

#57963

Purpose

Localization: Nucleus/Histones, Excitation: 549, Emission: 565

Depositor

Michael Davidson

Insert

H3 (H3C2 Human)

Use

Tags

mOrange2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
mOrange2-H4-23

Plasmid

#57964

Purpose

Localization: Nucleus/Histones, Excitation: 549, Emission: 565

Depositor

Michael Davidson

Insert

H4 (HIST1H4J Human)

Use

Tags

mOrange2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
PS-mOrange2-N1

Plasmid

#54849

Purpose

Localization: N1 Cloning Vector, Excitation: 548 / 634, Emission: 565 / 662

Depositor

Vladislav Verkhusha, Michael Davidson

Article

Subach et al J Am Chem Soc. 2012 Aug 17.

Type

Empty backbone

Use

Tags

PS-mOrange2

Expression

Mammalian

Mutation

Promoter

Available since

June 12, 2014

Availability

Academic Institutions and Nonprofits only
LI B-C mOrange

Plasmid

#54427

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

mOrange + 16 aa GS linker

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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mOrange2-ER-5

Plasmid

#57956

Purpose

Localization: Endoplasmic Reticulum, Excitation: 549, Emission: 565

Depositor

Michael Davidson

Insert

ER (CALR Human)

Use

Tags

mOrange2

Expression

Mammalian

Mutation

ER Signal Peptide

Promoter

CMV

Available since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
PS-mOrange2-C1

Plasmid

#54848

Purpose

Localization: C1 Cloning Vector, Excitation: 548 / 634, Emission: 565 / 662

Depositor

Vladislav Verkhusha, Michael Davidson

Article

Subach et al J Am Chem Soc. 2012 Aug 17.

Type

Empty backbone

Use

Tags

PS-mOrange2

Expression

Mammalian

Mutation

Promoter

Available since

June 11, 2014

Availability

Academic Institutions and Nonprofits only
mOrange2-H1-10

Plasmid

#57960

Purpose

Localization: Nucleus/Histones, Excitation: 549, Emission: 565

Depositor

Michael Davidson

Insert

H1 (H1f0 Mouse)

Use

Tags

mOrange2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
LI C-D mOrange c3

Plasmid

#54419

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

mOrange

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pM-LSSmOrange

Plasmid

#228619

Purpose

Mammalian expression of LSSmOrange and extra insert, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al bioRxiv [Preprint]. 2024 Oct 24:2024.10.

Insert

LSSmOrange

Use

Tags

Expression

Mammalian

Mutation

We introduced some silent mutations to avoid esse…

Promoter

CMV

Available since

Jan. 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pR-mOrange2

Plasmid

#228608

Purpose

Mammalian expression of mOrange2, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al bioRxiv [Preprint]. 2024 Oct 24:2024.10.

Insert

mOrange2

Use

Tags

Expression

Mammalian

Mutation

We introduced some silent mutations to avoid esse…

Promoter

CMV

Available since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pR-LSSmOrange

Plasmid

#228600

Purpose

Mammalian expression of LSSmOrange, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al bioRxiv [Preprint]. 2024 Oct 24:2024.10.

Insert

LSSmOrange

Use

Tags

Expression

Mammalian

Mutation

We introduced some silent mutations to avoid esse…

Promoter

CMV

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pM-mOrange2

Plasmid

#228627

Purpose

Mammalian expression of mOrange2 and extra insert, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al bioRxiv [Preprint]. 2024 Oct 24:2024.10.

Insert

mOrange2

Use

Tags

Expression

Mammalian

Mutation

We introduced some silent mutations to avoid esse…

Promoter

CMV

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pQLinkHD_LSSmOrange

Plasmid

#118860

Purpose

fluorescent protein expression with an N-terminal His-tag

Depositor

Marc Birtwistle

Article

Holzapfel et al ACS Comb Sci. 2018 Nov 12;20(11):653-659

Insert

LSSmOrange

Use

Tags

His-tag

Expression

Bacterial

Mutation

A44V,F84L, W145M, I165D, M167L, G202D substituti…

Promoter

Available since

Dec. 13, 2018

Availability

Academic Institutions and Nonprofits only

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LeGO-mOrange2

Plasmid

#85212

Purpose

Lentiviral vector expressing mOrange2 fluorescent protein driven by SFFV promoter

Depositor

Boris Fehse

Article

Mohme et al Mol Ther. 2017 Jan 18. pii: S1525-0016(1

Insert

mOrange2

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Jan. 18, 2017

Availability

Academic Institutions and Nonprofits only

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hCXCR4-LSSmOrange

Plasmid

#110197

Purpose

Encodes for human CXCR4 coding sequence tagged with the LSS-mOrange FP

Depositor

Sergi Padilla Parra

Article

Starling et al Nat Commun. 2023 Aug 17;14(1):4994. doi:

Insert

hCXCR4 (CXCR4 Human)

Use

Tags

LSSmOrange

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pActinin-LSSmOrange

Plasmid

#37139

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al J Am Chem Soc. 2012 Apr 24.

Insert

LSSmOrange

Use

Tags

human alpha-actinin

Expression

Mammalian

Mutation

A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

Promoter

CMV

Available since

June 5, 2012

Availability

Academic Institutions and Nonprofits only

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mOrange2-pBAD

Plasmid

#54531

Purpose

Localization: Protein Expression Vector , Excitation: 549, Emission: 565

Depositor

Roger Tsien, Michael Davidson, Nathan Shaner

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Type

Empty backbone

Use

Tags

6xHis and mOrange2

Expression

Bacterial

Mutation

Promoter

Available since

July 25, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge