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Addgene
Showing: 21 - 40 of 102 results

  1. PMmEos3.2

    Plasmid
    #203755
    Purpose
    Encodes the membrane anchor of Lyn including the first 10 residues of the N terminus, with 1 myristoylation and 1 palmitoylation, with mEos3.2 fused to the C terminus. Used as a fluorescent plasma membrane probe.
    Depositor
    Sarah Veatch
    Article
    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d
    Insert
    Lyn n-term (LYN Human)
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    203755-plasmid-map-sequence-id-403338

    Enlarge

  2. mEos3.2GG

    Plasmid
    #203756
    Purpose
    Encodes a modified version of the membrane anchor of KRas, including the last 16 residues of the C terminus, with mEos3.2 fused to the N terminus. The base pairs encoding the CAAX box for KRas were replaced by the CAAX box from rap1B, resulting in a geranylgeranylation instead of farnesylation. Used as a fluorescent plasma membrane probe.
    Depositor
    Sarah Veatch
    Article
    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d
    Insert
    KRas c-term (KRAS Human)
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    203756-plasmid-map-sequence-id-404785

    Enlarge

  3. mEos3.2FP

    Plasmid
    #203757
    Purpose
    Encodes the membrane anchor of NRas, including the last 10 residues of the C terminus with 1 farnesylation and 1 palmitoylation, with mEos3.2 fused to the N terminus. Used as a fluorescent plasma membrane probe.
    Depositor
    Sarah Veatch
    Article
    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d
    Insert
    NRas c-term (NRAS Human)
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    203757-plasmid-map-sequence-id-403337

    Enlarge

  4. mEos3.2FPP

    Plasmid
    #203758
    Purpose
    Encodes the membrane anchor of HRas, including the last 10 residues of the C terminus with 1 farnesylation and 2 palmitoylations, with mEos3.2 fused to the N terminus. Used as a fluorescent plasma membrane probe.
    Depositor
    Sarah Veatch
    Article
    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d
    Insert
    HRas c-term (HRAS Human)
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    203758-plasmid-map-sequence-id-431758

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  5. mEos3.2GPI

    Plasmid
    #203759
    Purpose
    Encodes the fluorescent protein mEos3.2 followed by the C-terminal sequence derived from CD58 encoding a GPI-attachment signal. Used as a fluorescent plasma membrane probe.
    Depositor
    Sarah Veatch
    Article
    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d
    Insert
    CD58 c-term (CD58 Human)
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    203759-plasmid-map-sequence-id-404788

    Enlarge

  6. mEos3.2-Actin-C-18

    Plasmid
    #57443
    Purpose
    Localization: Actin, Excitation: 507 / 572, Emission: 516 / 580
    Depositor
    Michael Davidson
    Insert
    Actin (ACTB Human)
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits

  7. mEos3.2-Calnexin-N-14

    Plasmid
    #57445
    Purpose
    Localization: Endoplasmic Reticulum, Excitation: 507 / 572, Emission: 516 / 580
    Depositor
    Michael Davidson
    Insert
    Calnexin (CANX Human)
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    I474N in Calnexin
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits

  8. mEos3.2-Calreticulin-N-16

    Plasmid
    #57446
    Purpose
    Localization: Endoplasmic Reticulum, Excitation: 507 / 572, Emission: 516 / 580
    Depositor
    Michael Davidson
    Insert
    Calreticulin (CALR Human)
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits

  9. mEos3.2-Caveolin-C-10

    Plasmid
    #57447
    Purpose
    Localization: Membrane, Excitation: 507 / 572, Emission: 516 / 580
    Depositor
    Michael Davidson
    Insert
    Caveolin (CAV1 Human)
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits

  10. mEos3.2-CCPB1-C-18

    Plasmid
    #57448
    Purpose
    Localization: Actin Capping Protein , Excitation: 507 / 572, Emission: 516 / 580
    Depositor
    Michael Davidson
    Insert
    CCPB1 (CAPZB Chicken)
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits

  11. mEos3.2-CCPB1-N-18

    Plasmid
    #57449
    Purpose
    Localization: Actin Capping Protein , Excitation: 507 / 572, Emission: 516 / 580
    Depositor
    Michael Davidson
    Insert
    CCPB1
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits
    57449-plasmid-map-sequence-id-216841

    Enlarge

  12. mEos3.2-CENPB-N-22

    Plasmid
    #57451
    Purpose
    Localization: Nucleus/Centromeres, Excitation: 507 / 572, Emission: 516 / 580
    Depositor
    Michael Davidson
    Insert
    CENPB (CENPB Human)
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    aa 1-169 of CENPB (DNA Binding Domain)
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits
    57451-plasmid-map-sequence-id-203801

    Enlarge

  14. mEos3.2-Cx43-7

    Plasmid
    #57453
    Purpose
    Localization: Gap Junctions, Excitation: 507 / 572, Emission: 516 / 580
    Depositor
    Michael Davidson
    Insert
    Cx43 (Gja1 Rat)
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits
    57453-plasmid-map-sequence-id-210795

    Enlarge

  16. mEos3.2-Farnesyl-5

    Plasmid
    #57456
    Purpose
    Localization: Membrane, Excitation: 507 / 572, Emission: 516 / 580
    Depositor
    Michael Davidson
    Insert
    Farnesyl (HRAS Human)
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    encodes final 20 aa of NM_001130442.1
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits
    57456-plasmid-map-sequence-id-393325

    Enlarge

  17. mEos3.2-H2B-C-10

    Plasmid
    #57458
    Purpose
    Localization: Nucleus/Histones, Excitation: 507 / 572, Emission: 516 / 580
    Depositor
    Michael Davidson
    Insert
    H2B (H2BC11 Human)
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    D26G and V119I in H2B
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits

  18. mEos3.2-H2B-6

    Plasmid
    #57459
    Purpose
    Localization: Nucleus/Histones, Excitation: 507 / 572, Emission: 516 / 580
    Depositor
    Michael Davidson
    Insert
    H2B (H2BC11 Human)
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    D26G and V119I in H2B
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits

  19. mEos3.2-Homer1-C-18

    Plasmid
    #57460
    Purpose
    Localization: Membrane Adaptor, Excitation: 507 / 572, Emission: 516 / 580
    Depositor
    Michael Davidson
    Insert
    Homer1
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits

  20. mEos3.2-Homer1-N-18

    Plasmid
    #57461
    Purpose
    Localization: Membrane Adaptor, Excitation: 507 / 572, Emission: 516 / 580
    Depositor
    Michael Davidson
    Insert
    Homer1
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 21 - 40 of 102 results