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iRFP713-FRB-Fis1tail

Plasmid

#220080

Purpose

FRB targeted to the mitochondria with the tail of the mitochondrial outer membrane protein Fis1 with an iRFP fluorophore

Depositor

Gerry Hammond

Article

Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

Insert

FRB-Fis1tail

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-iRFP713-ORP9-PH

Plasmid

#214269

Purpose

Mammalian expression of near-IR fluorescent PI4P reporter iRFP713-ORP9-PH

Depositor

Shinya Tsukiji

Article

Ajiki et al RSC Chemical Biology

Insert

iRFP713-ORP9(1-111) (Osbpl9 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-miRFP713

Plasmid

#197208

Purpose

Expresses the protein of miRFP713 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

miRFP713

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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iRFP713-N2-Frankenbody-Flag

Plasmid

#175291

Purpose

Visualize endogeneous loci coupled with ZF probes harboring 10xFLAG tag

Depositor

Tim Stasevich

Article

Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

Insert

anti-FLAG frankenbody fused with iRFP713

Use

Tags

iRFP713

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 3, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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iRFP713-FRB-PIP5K1A

Plasmid

#202743

Purpose

expresses recruitable fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

iRFP713:MTOR(2021-2113):GGSA2:PIP5K1A (PIP5K1A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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plex_307-ccdB-iRFP713

Plasmid

#201140

Purpose

pLEX_307 plasmid with iRFP713 marker

Depositor

Alejandro Chavez

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF-1α

Available since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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iRFP713-C1-SNAP-Fis1tail

Plasmid

#220067

Purpose

allows for a reversible dimerization system to be targeted at the mitochondria with an iRFP fluorophore

Depositor

Gerry Hammond

Article

Doyle et al Contact (Thousand Oaks). 2024 Feb 22;7:2

Insert

SNAP-Fis1tail

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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plex_306-ccdB-iRFP713

Plasmid

#201132

Purpose

pLEX_306 plasmid with iRFP713 marker

Depositor

Alejandro Chavez

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-iRFP713

Plasmid

#197209

Purpose

Expresses the protein of iRFP713 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

iRFP713

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pSF11-wmiRFP713-T2A-HO1

Plasmid

#197246

Purpose

Expresses the protein of wmiRFP713-T2A-HO1 in neurons of C. elegans

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

wmiRFP713-T2A-HO1

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pSB700 lenti gRNA iRFP713_zhang2.0

Plasmid

#167916

Purpose

Lentiviral vector for expressing U6 MS2-sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-hSyn1-eNpHR3.0-iRFP713

Plasmid

#197594

Purpose

Lentiviral transfer vector for the neuronal expression of eNpHR3.0-iRFP713 (halorhodopsin for the inhibition of neuronal activity)

Depositor

Patrik Verstreken

Insert

eNpHR3.0-iRFP713

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hSynI

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pSB700 lenti gRNA iRFP713

Plasmid

#167908

Purpose

Lentiviral vector for expressing U6 sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-miRFP713-HO1

Plasmid

#197255

Purpose

Expresses the protein of miRFP713 and HO1 in bacteria

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

miRFP713

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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iRFP713-FRB-Mss4-Kina

Plasmid

#202744

Purpose

expresses recruitable fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

iRFP713:MTOR(2021-2113):GGSA2:S. cerevisiae Mss4 (377-756) (MSS4 Budding Yeast)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pPHR-iRFP713-NLS-VP16

Plasmid

#103823

Purpose

Soluble BLInCR transcription activator (transactivation domain of viral VP16 protein) that is recruited to 'localizer' sites upon blue light illumination

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

PHR-iRFP713-VP16 (CRY2 Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

VP16: G126T (silent, G42G)

Promoter

CMV

Available since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pSF11-wiRFP713-T2A-HO1

Plasmid

#197247

Purpose

Expresses the protein of wiRFP713-T2A-HO1 in neurons of C. elegans

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

wiRFP713-T2A-HO1

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAD-HisD-iRFP713-HO1

Plasmid

#197256

Purpose

Expresses the protein of iRFP713 and HO1 in bacteria

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

iRFP713

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGGS-eDHFR(69K6)-iRFP713

Plasmid

#178855

Purpose

Mammalian expression of eDHFR(69K6)-iRFP713

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

eDHFR(69K6)-iRFP713

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR2-L3-SfoI-iRFP713-PBL-R1

Plasmid

#141023

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

iRFP713

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge