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Plasmid#164000PurposeMammalian expression vector encoding codon-optimized human NLRP1 CARD (dimer interface mutation) with a C-terminal mCherry tag for imagingDepositorInsertNLRP1 (NLRP1 Human)
UseTagsmCherryExpressionMammalianMutationM1457APromoterAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry Y1445A
Plasmid#163999PurposeMammalian expression vector encoding codon-optimized human NLRP1 CARD (dimer interface mutation) with a C-terminal mCherry tag for imagingDepositorInsertNLRP1 (NLRP1 Human)
UseTagsmCherryExpressionMammalianMutationY1445APromoterAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry R1427E
Plasmid#163998PurposeMammalian expression vector encoding codon-optimized human NLRP1 CARD (filament-deficient mutation) with a C-terminal mCherry tag for imagingDepositorInsertNLRP1 (NLRP1 Human)
UseTagsmCherryExpressionMammalianMutationR1427EPromoterAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry E1414R
Plasmid#163997PurposeMammalian expression vector encoding codon-optimized human NLRP1 CARD (filament-deficient mutation) with a C-terminal mCherry tag for imagingDepositorInsertNLRP1 (NLRP1 Human)
UseTagsmCherryExpressionMammalianMutationE1414RPromoterAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry E1411R
Plasmid#163996PurposeMammalian expression vector encoding codon-optimized human NLRP1 CARD (filament-deficient mutation) with a C-terminal mCherry tag for imagingDepositorInsertNLRP1 (NLRP1 Human)
UseTagsmCherryExpressionMammalianMutationE1411RPromoterAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry E1397R
Plasmid#163994PurposeMammalian expression vector encoding codon-optimized human NLRP1 CARD (filament-deficient mutation) with a C-terminal mCherry tag for imagingDepositorInsertNLRP1 (NLRP1 Human)
UseTagsmCherryExpressionMammalianMutationE1397RPromoterAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry R1392E
Plasmid#163993PurposeMammalian expression vector encoding codon-optimized human NLRP1 CARD (filament-deficient mutation) with a C-terminal mCherry tag for imagingDepositorInsertNLRP1 (NLRP1 Human)
UseTagsmCherryExpressionMammalianMutationR1392EPromoterAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry R1386E
Plasmid#163992PurposeMammalian expression vector encoding codon-optimized human NLRP1 CARD (filament-deficient mutation) with a C-terminal mCherry tag for imagingDepositorInsertNLRP1 (NLRP1 Human)
UseTagsmCherryExpressionMammalianMutationR1386EPromoterAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac_Dual-GST-TEV-SINTBAD-EGFP (codon optimized Sf9)
Plasmid#198035PurposeUsed for the expression and purification of EGFP labelled SINTBAD (SMC Internal No. 1613)DepositorInsertSINTBAD (TBKBP1 Human)
UseTagsEGFP and GST-TEVExpressionInsectMutationPromoterAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 NLRP1-delPYD-delCARD-TEV-GFP-FLAG codon-optimized
Plasmid#166834PurposeExpression and purification of NLRP1 (without the PYD and CARD) from mammalian cellsDepositorInsertNLRP1 (NLRP1 Human)
UseTagsTEV-GFP-FLAGExpressionMammalianMutationPromoterCMVAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-Tight-Pur-Trim69 Full length, isoform A (codon optimized)
Plasmid#199566PurposeStable and dox. inducible expression of Trim69 Full length, isoform A (codon optimized) after retroviral-mediated gene transferDepositorInsertTrim69 Full length, isoform A (codon optimized) (TRIM69 Human)
UseRetroviral; Allows for puromycin selectionTagsFlagExpressionMutationcodon optimizedPromoterAvailable sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
RRL.sin.cPPT.SFFV/Flag-codon-optimized-NCOA7 variant 6.IRES-puro.WPRE (CG584)
Plasmid#139444PurposeLentiviral vector to ectopically express human codon-optimized NCOA7 isoform 4DepositorInsertFLAG-tag codon-optimized NCOA7 variant 6 (coding for isoform 4, the short, interferon-inducble isoform) (NCOA7 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationsequence codon-optimizedPromoterSFFVAvailable sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC57-ITR2-H1- sgCTG Gollum-eCMV-SaSpD10A (Neuron codon optimization)-3XFLAG-SV40 NLS-ITR2
Plasmid#210735PurposeCoding for SaSp D10A Cas9 alongside Gollum sgRNA targeting CAG repeatsDepositorInsertsSaSp D10A Cas9
Gollum sgCAG
UseCRISPRTags3xFLAG, SV40 NLS, PolyA signalExpressionMammalianMutationD10A, N terminal Sa Cas9, C-terminal Sp Cas9PromoterH1 and eCMVAvailable sinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC57-ITR2-H1- sgCTG Merry-eCMV-SaSpD10A+linker (Neuron codon optimization)-3XFLAG-SV40 NLS-ITR2
Plasmid#210736PurposeCoding for SaSp D10A Cas9 alongside Merry sgRNA targeting CAG repeatsDepositorInsertsSaSp D10A Cas9
Merry sgCAG
UseCRISPRTags3xFLAG, SV40 NLS, PolyA signalExpressionMammalianMutationD10A, N-terminal Sa Cas9, C-terminal Sp Cas9PromoterH1 and eCMVAvailable sinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBS-Nvec-codonopti-Cas9-2xNLS
Plasmid#141108PurposePlasmid containing insert for the IVT of nuclear localized Cas9 mRNA with codon optimization for Nematostella vectensisDepositorInsertNematostella codon optimized Cas9
UseUnspecifiedTagsExpressionMutationAll amino acids coded for by codons optimized for…PromoterT7Available sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMK-Lux-G codon opt. Nt chloroplasts
Plasmid#123568PurposeMoChlo kitDepositorInsertLux-G codon optimized Nt chloroplasts
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK-Lux-E codon opt. Nt chloroplasts
Plasmid#123567PurposeMoChlo kitDepositorInsertLux-E codon optimized Nt chloroplasts
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK-Lux-B codon opt. Nt chloroplasts
Plasmid#123566PurposeMoChlo kitDepositorInsertLux-B codon optimized Nt chloroplasts
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK-Lux-A codon opt. Nt chloroplasts
Plasmid#123565PurposeMoChlo kitDepositorInsertLux-A codon optimized Nt chloroplasts
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK-Lux-D codon opt. Nt chloroplasts
Plasmid#123564PurposeMoChlo kitDepositorInsertLux-D codon optimized Nt chloroplasts
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only