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Showing: 21 - 40 of 70 results

  1. pECNUS2

    Plasmid
    #184885
    Purpose
    ABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery
    Depositor
    Eunyoung Chae
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    RPS5A
    Available since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    184885-plasmid-map-sequence-id-435252

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  2. pECNUS3

    Plasmid
    #184886
    Purpose
    ABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery
    Depositor
    Eunyoung Chae
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    RPS5A
    Available since
    July 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    184886-plasmid-map-sequence-id-437894

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  3. pECNUS1

    Plasmid
    #184884
    Purpose
    ABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery
    Depositor
    Eunyoung Chae
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    RPS5A
    Available since
    July 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    184884-plasmid-map-sequence-id-437893

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  4. pUS250

    Plasmid
    #198322
    Purpose
    Cumate-inducible gene expression in diverse gram negative bacteria. Features also include mobilisation via oriT, a gigantic multiple cloning site, and blue/white screening via amilCP chromoprotein
    Depositor
    Nicholas Coleman
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    cumate-inducible
    Available since
    April 12, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    198322-plasmid-map-sequence-id-390064

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  8. pUPD2

    Plasmid
    #165856
    Purpose
    Universal Part domesticator vector v2 used for integration (domestication) of new DNA elements in the GB2.0 cloning system uses blue-white screening
    Depositor
    Koen Venken
    Article
    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    165856-plasmid-map-sequence-id-346785

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  9. pVD2

    Plasmid
    #165857
    Purpose
    universal Vector Domesticator vector v2 used in the integration (domestication) of new vector backbone parts in the GB2.0 cloning system. Uses blue-white screening method
    Depositor
    Koen Venken
    Article
    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    165857-plasmid-map-sequence-id-335645

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  11. pR6Kg-Alpha1

    Plasmid
    #165862
    Purpose
    Alpha level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived) uses blue-white screening
    Depositor
    Koen Venken
    Article
    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    165862-plasmid-map-sequence-id-335009

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  12. Level 1 P4 TaU6 guide acceptor

    Plasmid
    #165600
    Purpose
    GoldenGate (MoClo) Level 1 Position 4 TaU6 guide acceptor plasmid
    Depositor
    John Innes Centre - Crop Transformation and Genome Editing Group, Wendy Harwood
    Article
    Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.
    Insert
    TaU6 promoter::LacZ::sgRNA scaffold
    Use
    CRISPR and Synthetic Biology; Sgrna acceptor with…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    165600-plasmid-map-sequence-id-331273

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  13. Level 1 P3 TaU6 guide acceptor

    Plasmid
    #165599
    Purpose
    GoldenGate (MoClo) Level 1 Position 3 TaU6 guide acceptor plasmid
    Depositor
    John Innes Centre - Crop Transformation and Genome Editing Group, Wendy Harwood
    Article
    Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.
    Insert
    TaU6 promoter::LacZ::sgRNA scaffold
    Use
    CRISPR and Synthetic Biology; Sgrna acceptor with…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    165599-plasmid-map-sequence-id-331270

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  19. pColE1-AlphaOmega

    Plasmid
    #165867
    Purpose
    Terminal pBlueScript derived high-copy GB Cloning vector with both Alpha and Omega Entry points requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived)uses blue-white screening
    Depositor
    Koen Venken
    Article
    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    165867-plasmid-map-sequence-id-345193

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Showing: 21 - 40 of 70 results