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Plasmid#206956PurposeAAV plasmid for CaMKIIa driven co-expression of mScarlet-I and 2M-Qt-FLAG EMcapsulinsDepositorInsertmScarlet-I, MmMT3, QtEnc
UseAAVTagsFLAGExpressionMutationPromoterAvailable sinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
Qt-FLAG-TAA-rt20s-antiGFP
Plasmid#206955PurposeMammalian expression of Qt EMcapsulins targeting GFP with stoichiometry control (~6% surface decoration)DepositorInsertQtEnc, antiGFP-intrabody
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
1M-Qt-FLAG-TGA-rt20s-antiGFP
Plasmid#206953PurposeMammalian expression of 1M-Qt EMcapsulins targeting GFP with stoichiometry control (~20% surface decoration)DepositorInsertMmMT3, QtEnc, antiGFP-intrabody
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
sfGFP-SasG(2G)-mCherry
Plasmid#206944PurposeMammalian expression of heterobifunctional SasG linkers (2G)DepositorInsertsfGFP-SaSasG (2G)-mCherry
UseTagsmCherry and sfGFPExpressionMammalianMutationPromoterAvailable sinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
1M-Mx-FLAG
Plasmid#206941PurposeMammalian Expression of 1M-Mx-FLAG EMcapsulinsDepositorInsertMmMT3, MxEnc
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
1M-Qt-FLAG
Plasmid#206938PurposeMammalian Expression of 1M-Qt-FLAG EMcapsulinsDepositorInsertMmMT3, QtEnc
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
1M-Tm-BC2
Plasmid#206943PurposeMamamlian Expression of 1M-Tm-BC2Tag EMcapsulinsDepositorInsertMmMT3, TmEnc
UseTagsBC2ExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
3M-Qt-FLAG
Plasmid#206940PurposeMammalian Expression of 3M-Qt-FLAG EMcapsulinsDepositorInsertMmMT3, SeSmtA, TaEC1, QtEnc
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
2M-Qt-FLAG
Plasmid#206939PurposeMammalian Expression of 2M-Qt-FLAG EMcapsulinsDepositorInsertMmMT3, QtEnc
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pINSPECT:NEAT1-total_SigP:NLuc
Plasmid#197267PurposeDonor plasmid for CRISPR/Cas9-mediated insertion of INSPECT into human NEAT1 locus (both isoforms). Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette.DepositorInsertNEAT1-total homology arms
UseCRISPR, Luciferase, Synthetic Biology, and Unspec…TagsExpressionMammalianMutationPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pINSPECT_SigP:NLuc
Plasmid#197263PurposeCloning Backbone for INSPECT expression. Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette. Clone homology arms via Esp3I.DepositorTypeEmpty backboneUseCRISPR, Luciferase, Synthetic Biology, and Unspec…TagsExpressionMammalianMutationPromoterAvailable sinceApril 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEXSISERS_NLuc_FRT-PuroR
Plasmid#177865PurposeCloning Backbone for NanoLuc-luciferase-based EXSISERS containing FRT-sites flanked PuroR cassetteDepositorInsertNLuc-based EXSISERS
UseCRISPR, Synthetic Biology, and UnspecifiedTagsOLLASExpressionMammalianMutationPromoterAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEXSISERS_BSD_FRT-PuroR
Plasmid#177867PurposeCloning Backbone for Blasticidin-S-deaminase-based EXSISERS containing FRT-sites flanked PuroR cassetteDepositorInsertBSD-based EXSISERS
UseCRISPR, Luciferase, Synthetic Biology, and Unspec…TagsOLLASExpressionMammalianMutationPromoterAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEXSISERS_SurfaceHaloTag_FRT-PuroR
Plasmid#177869PurposeCloning Backbone for Surface-HaloTag-based EXSISERS containing FRT-sites flanked PuroR cassette; clone homology arms via BbsI (or BpiI).DepositorInsertSurface-HaloTag-based EXSISERS
UseCRISPR, Synthetic Biology, and UnspecifiedTagsExpressionMammalianMutationPromoterAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only