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pGWB602-tkUnaG

Plasmid

#221661

Purpose

For Agrobacterium-mediated transformation. Plastid(thylakoid membrane)-localized mCherry-UnaG.

Depositor

Yutaka Kodama

Article

Ishikawa et al Plant Cell Physiol. 2024 Feb 27:pcae017.

Insert

mCherry-UnaG flanked with the full-length coding sequence for Arabidopsis BESTROPHIN-LIKE PROTEIN2

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pSC-cUnaG

Plasmid

#207631

Purpose

A plasmid encoding photocaged SpyCatcher (pSC) fused to C-terminal UnaG fragment.

Depositor

Cole DeForest

Article

Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

Insert

photocaged SpyCatcher-cUnaG

Use

Tags

6xHis

Expression

Bacterial

Mutation

Amber stop codon at SC's critical lysine

Promoter

T7

Available since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pGWT35S-TP-UnaG

Plasmid

#203484

Purpose

For particle bombardment. A plastid transit peptide fused UnaG under the control of the cauliflower mosaic virus (CaMV) 35S promoter.

Depositor

Yutaka Kodama

Article

Ishikawa et al Sci Adv. 2023 Jun 9;9(23):eadh4787. doi:

Insert

Plastid transit peptide fused UnaG

Use

Tags

Expression

Bacterial and Plant

Mutation

Promoter

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-HRE-dUnaG

Plasmid

#124372

Purpose

UnaG fluorescent protein reporter for hypoxia-induced factor (HIF) signaling

Depositor

Roland Friedel

Article

Sattiraju et al Immunity. 2023 Jul 7:S1074-7613(23)00274

Insert

HRE-dUnaG

Use

Lentiviral

Tags

PEST-degron and Myc tag

Expression

Mutation

Promoter

HRE (HIF responsive element 5x + CMV minimal prom…

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3_UnaG-Flag-Sec61B

Plasmid

#83413

Purpose

Expressed N-term tagged UnaG on Sec61B

Depositor

Hyun-Woo Rhee

Article

Park et al ACS Chem Biol. 2016 May 27.

Insert

UnaG-Sec61B (SEC61B Synthetic, Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

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pRDAX-381 HA UnaG

Plasmid

#130132

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

UnaG

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-UnaG-pA (pE091)

Plasmid

#149354

Purpose

Mammalian Expression vector for UnaG

Depositor

Ralf Kuehn

Article

Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

Insert

UnaG

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pSC-cUnaG-LPETG

Plasmid

#207635

Purpose

A plasmid encoding photocaged SpyCatcher (pSC) fused to C-terminal UnaG fragment and LPETG sortase recognition sequence.

Depositor

Cole DeForest

Article

Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

Insert

photocaged SpyCatcher-GGSGGGSG-cUnaG-LPETG

Use

Tags

6xHis

Expression

Bacterial

Mutation

Amber stop codon at SC's critical lysine

Promoter

T7

Available since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pGWB511-TP-UnaG-FLAG

Plasmid

#203479

Purpose

For Agrobacterium transformation. A plastid transit peptide fused UnaG-FLAG under the control of the cauliflower mosaic virus (CaMV) 35S promoter.

Depositor

Yutaka Kodama

Article

Ishikawa et al Sci Adv. 2023 Jun 9;9(23):eadh4787. doi:

Insert

Plastid transit peptide fused UnaG-FLAG

Use

Tags

Expression

Bacterial and Plant

Mutation

Promoter

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pGWB602-TP-mCherry-UnaG

Plasmid

#203483

Purpose

For Agrobacterium transformation. A plastid transit peptide fused mCherry-UnaG under the control of the cauliflower mosaic virus (CaMV) 35S promoter.

Depositor

Yutaka Kodama

Article

Ishikawa et al Sci Adv. 2023 Jun 9;9(23):eadh4787. doi:

Insert

Plastid transit peptide fused mCherry-UnaG

Use

Tags

Expression

Bacterial and Plant

Mutation

Promoter

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRDAX-379 FLAG UnaG

Plasmid

#130131

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

UnaG

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-UnaG-P2A-FusionRed

Plasmid

#191109

Purpose

Mammalian cell line expression plasmids for production of UnaG and FusionRed proteins

Depositor

Kiryl Piatkevich

Article

Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

Insert

UnaG

Use

Tags

FusionRed

Expression

Mammalian

Mutation

See depositor comments below

Promoter

chicken β-actin promoter

Available since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pRDAX-460 N-Tag UnaG[1] L20 FKBP EBPF2

Plasmid

#129927

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

FKBP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pRDAX-475 C-Tag UnaG[2] L10 FRB mCherry

Plasmid

#129918

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

FRB

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 11, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pRDAX-473 C-Tag UnaG[1] L32 FRB EBPF2

Plasmid

#129916

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

FRB

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pRDAX-474 N-Tag UnaG[1] L32 FRB EBPF2

Plasmid

#129917

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

FRB

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pRDAX-476 N-Tag UnaG[2] L10 FRB mCherry

Plasmid

#129919

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

FRB

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pRDAX-477 C-Tag UnaG[2] L20 FRB mCherry

Plasmid

#129920

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

FRB

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pRDAX-478 N-Tag UnaG[2] L20 FRB mCherry

Plasmid

#129921

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

FRB

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pRDAX-479 C-Tag UnaG[2] L32 FRB mCherry

Plasmid

#129922

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

FRB

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge