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Showing: 21 - 40 of 1600 results

  1. pDule2-3-nitroTyrosine (A7)

    Plasmid
    #174079
    Purpose
    Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the third generation Mj 3NY (A7) synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Article
    Beyer et al J Mol Biol. 2020 Jul 24;432(16):4690-470
    Insert
    3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Y32H H70T D158H I159A L162R
    Promoter
    GlnRS (constitutive)
    Available since
    Aug. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    174079-plasmid-map-sequence-id-341130

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  4. 60-p3E_INTRON-EmptyRNAi

    Plasmid
    #163380
    Purpose
    p3E-Vector (Gateway R2-L3) for Tol2-kit cloning. Vector for performing RNAi (gene silencing - knockdown) in zebrafish via cloning and expression of synthetic miR(s).
    Depositor
    Jean Giacomotto
    Type
    Empty backbone
    Use
    RNAi
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    163380-plasmid-map-sequence-id-322413

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  6. pDule-3-nitroTyrosine (A7)

    Plasmid
    #174078
    Purpose
    Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the third generation Mj 3NY (A7) synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Article
    Beyer et al J Mol Biol. 2020 Jul 24;432(16):4690-470
    Insert
    3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Y32H H70T D158H I159A L162R
    Promoter
    GlnRS (constitutive)
    Available since
    Oct. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    174078-plasmid-map-sequence-id-345712

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  8. pBridge-tyrosinase.σ2

    Plasmid
    #197415
    Purpose
    Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-2 σ2 fusion protein in yeast (yeast three-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.
    Inserts
    tyrosinase cytosolic tail
    AP-2 σ2
    Use
    Tags
    GAL4-DNA binding domain fragment, HA tag, and nuc…
    Expression
    Yeast
    Mutation
    encodes R42G substitution, contains silent substi…
    Promoter
    ADH1 and MET25
    Available since
    March 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197415-plasmid-map-sequence-id-388893

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  9. pDule-Mb haloTyrRS C6

    Plasmid
    #160377
    Purpose
    C6 HaloTyrosine tRNA synthatase and cognate amber suppressing tRNA derived from M. barkeri.
    Depositor
    Ryan Mehl
    Article
    Jang et al ACS Chem Biol. 2020 Feb 21;15(2):562-574
    Insert
    C6 HaloTyrosine tRNA synthatase
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    L270S, Y271L, N311G, C313T
    Promoter
    Available since
    Nov. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    160377-plasmid-map-sequence-id-315316

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  10. pDule-3-nitroTyrosine (5B)

    Plasmid
    #85498
    Purpose
    Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Article
    Cooley et al Biochemistry. 2014 Apr 1;53(12):1916-24.
    Insert
    3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32H H70C D158S I159A L162R
    Promoter
    lpp (constitutive)
    Available since
    Jan. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    85498-plasmid-map-sequence-id-169862

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  11. pDule2-3-nitroTyrosine (5B)

    Plasmid
    #85499
    Purpose
    Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Article
    Cooley et al Biochemistry. 2014 Apr 1;53(12):1916-24.
    Insert
    3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Y32H H70C D158S I159A L162R
    Promoter
    lpp (constitutive)
    Available since
    Jan. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    85499-plasmid-map-sequence-id-169856

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  14. pBridge-tyrosinase.σ1A

    Plasmid
    #197381
    Purpose
    Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-1 σ1A fusion protein in yeast (yeast three-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.
    Inserts
    tyrosinase cytosolic tail
    AP-1 σ1A
    Use
    Tags
    GAL4-DNA binding domain fragment, HA tag, and SV4…
    Expression
    Yeast
    Mutation
    Contains an extra 42 nucleotides encoding 14 resi…
    Promoter
    ADH1 and MET25
    Available since
    March 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197381-plasmid-map-sequence-id-388878

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  15. BcatMut-Tyr654

    Plasmid
    #24199
    Depositor
    David Rimm
    Insert
    beta-catenin (CTNNB1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Y654F
    Promoter
    Available since
    March 23, 2010
    Availability
    Academic Institutions and Nonprofits only

  16. BcatMut-Tyr489

    Plasmid
    #24197
    Depositor
    David Rimm
    Insert
    beta-catenin (CTNNB1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Y489F
    Promoter
    Available since
    March 15, 2010
    Availability
    Academic Institutions and Nonprofits only

  17. BcatMut-Tyr604

    Plasmid
    #24196
    Depositor
    David Rimm
    Insert
    beta-catenin (CTNNB1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Y604F
    Promoter
    Available since
    March 9, 2010
    Availability
    Academic Institutions and Nonprofits only

  18. BcatMut-Tyr670

    Plasmid
    #24198
    Depositor
    David Rimm
    Insert
    beta-catenin (CTNNB1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Y670F
    Promoter
    Available since
    March 9, 2010
    Availability
    Academic Institutions and Nonprofits only

  19. BcatMut-Tyr432

    Plasmid
    #24200
    Depositor
    David Rimm
    Insert
    beta-catenin (CTNNB1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Y432F
    Promoter
    Available since
    March 15, 2010
    Availability
    Academic Institutions and Nonprofits only

  20. pEF2-FL-hTyro_gR1

    Plasmid
    #225995
    Purpose
    Expresses a chimeric contruct having N-terminal human Tyro3 and C-terminal human Interferon Gamma Receptor
    Depositor
    Raymond Birge, Sergei Kotenko
    Article
    Tsou et al J Biol Chem. 2014 Sep 12;289(37):25750-6
    Insert
    human Tyro3 N-Terminal human Interferon Gamma R1 Receptor C-Terminal fusion (TYRO3, IFNGR1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1-a
    Available since
    Nov. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    225995-plasmid-map-sequence-id-454328

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Showing: 21 - 40 of 1600 results