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pCFJ2307 - [1-2] ENTR - Fluor - ce-tagRFP-T(1 intron, syntrons(3), NLS(2), no_atg, no_stop)

Plasmid

#159856

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-tagRFP-T(1 intron, syntrons(3), NLS(2), no_atg, no_stop)

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ2308 - [1-2] ENTR - Fluor - mNeon(1 intron, syntrons(3), NLS(2), no_atg, no_stop)

Plasmid

#159852

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - mNeon(1 intron, syntrons(3), NLS(2), no_atg, no_stop)

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pDGB3_o1_LIR-2nd intron-3'eGFP-T35S-SIR-p35S-5'eGFP-1st intron-LIR (GB5177)

Plasmid

#225442

Purpose

BeYDV LIR:2nd intron + 3'eGFP +T35S:BeYDV SIR + p35S + 5'eGFP + 1st intron:BeYDV LIR for INPACT configuration.

Depositor

Diego Orzaez

Insert

LIR:2nd intron:3'eGFP:T35S:SIR:p35S:5'eGFP:1st intron:LIR

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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CAG-loxP-3xpA-loxP-H2B-sfGFP+pCI syntron-T2A-tdTomato+RbG and HsbG syntrons

Plasmid

#172480

Purpose

H2B-sfGFP with a synthetic intron from the pCI expression vector, T2A, and tdTomato with synthetic introns derived from the rabbit beta-globin gene and the human beta-globin gene

Depositor

Mario Capecchi

Insert

H2B-sfGFP-T2A-tdTomato

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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AAV9retro

Plasmid

#224687

Purpose

AAV packaging plasmid expressing Rep/Cap genes

Depositor

Zhigang He

Article

Zhang et al Cell. 2024 May 3:S0092-8674(24)00447-1.

Insert

Rep2/Cap9retro

Use

AAV

Tags

Expression

Mutation

insertion of 10-mer peptide from AAV2retro (LADQD…

Promoter

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pLIX403_control_APOBEC_HA_P2A_mRuby_Capture1

Plasmid

#183951

Purpose

Inducible lentiviral expression, TRE-control-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTA

Depositor

Eugene Yeo

Article

Brannan et al Nat Methods. 2021 May;18(5):507-519. doi

Insert

APOBEC1 (Apobec1 Rat)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE promoter, Tet ON

Available since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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Cilantro 2

Plasmid

#74450

Purpose

Can clone in a C2H2 zinc finger via BsmBI restriction sites and monitor post-translational degradation using EGFP:mCherry ratio (Lentiviral, PGK.BsmBICloneSite-EGFP.IRES.mCherry.cppt.EF1a.PuroR)

Depositor

Benjamin Ebert

Article

Slabicki et al Nature. 2020 Sep;585(7824):293-297. doi:

Type

Empty backbone

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Promoter

Available since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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pRetroNeo

Plasmid

#21740

Depositor

Clare Huxley

Article

Magin-Lachmann et al BMC Biotechnol. 2003 Feb 3. 3(1):2.

Type

Empty backbone

Use

N/a

Tags

Expression

Mutation

Promoter

Available since

Sept. 28, 2009

Availability

Academic Institutions and Nonprofits only

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pBJ089_zACNE20_myl7_Voltron1_P2A_jGCaMP7f

Plasmid

#209283

Purpose

Expresses Voltron1 and jGCaMP7f in the zebrafish heart

Depositor

Adam Cohen

Article

Jia et al Nature. 2023 Oct;622(7981):149-155. doi:

Insert

Voltron1_P2A_jGCaMP7f

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

zACNE20_myl7

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pEmpty_Control

Plasmid

#162607

Purpose

Empty control for Cas9 experiments and to clone new guide RNAs for paper

Depositor

Lars Steinmetz

Article

Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

Insert

Tef1-Cas9 with RPL25 Intron

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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GPR_control

Plasmid

#216222

Purpose

GFP P2A RFP control reporter

Depositor

Rebecca Voorhees

Article

Inglis et al J Cell Sci. 2023 May 15;136(10):jcs26121

Insert

GFP P2A RFP_stop

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Seattle_IPDA_control_1_001

Plasmid

#167347

Purpose

ddPCR gating control for droplets containing all 5 targets (2 in pol)

Depositor

Florian Hladik

Article

Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

Insert

pol_gag_tat_env

Use

Other

Tags

Expression

Mutation

Promoter

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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Seattle_IPDA_control_9_003

Plasmid

#167349

Purpose

ddPCR gating control for droplets containing either gag+env targets OR tat + env targets

Depositor

Florian Hladik

Article

Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

Insert

gag_tat_env

Use

Other

Tags

Expression

Mutation

Promoter

Available since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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Seattle_IPDA_control_6_002

Plasmid

#167348

Purpose

ddPCR gating control for droplets containing either gag+5'pol targets OR 3'pol+tat targets

Depositor

Florian Hladik

Article

Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

Insert

pol_gag_tat

Use

Other

Tags

Expression

Mutation

Promoter

Available since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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Seattle_IPDA_control_29_006

Plasmid

#167352

Purpose

ddPCR gating control for droplets containing either env+5'pol targets OR env+3'pol targets

Depositor

Florian Hladik

Article

Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

Insert

pol_env

Use

Other

Tags

Expression

Mutation

Promoter

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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Seattle_IPDA_control_14_004

Plasmid

#167350

Purpose

ddPCR gating control for droplets containing the env target

Depositor

Florian Hladik

Article

Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

Insert

env

Use

Other

Tags

Expression

Mutation

Promoter

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Seattle_IPDA_control_32_007

Plasmid

#167353

Purpose

ddPCR gating control for droplets containing 5'pol or 3'pol targets

Depositor

Florian Hladik

Article

Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

Insert

pol

Use

Other

Tags

Expression

Mutation

Promoter

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Seattle_IPDA_control_17_005

Plasmid

#167351

Purpose

ddPCR gating control for droplets containing gat and tat targets

Depositor

Florian Hladik

Article

Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

Insert

gat_tat

Use

Other

Tags

Expression

Mutation

Promoter

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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HT142_pAAV_EF1a_fDiO_Voltron2

Plasmid

#208607

Purpose

Expressing Voltron2 in a Flp-dependent manner

Depositor

Adam Cohen

Insert

Voltron2

Use

AAV

Tags

Halo tag

Expression

Mutation

Promoter

EF1a

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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GPR_control_lenti

Plasmid

#216223

Purpose

GFP P2A RFP control reporter in a lenti backbone

Depositor

Rebecca Voorhees

Article

Inglis et al J Cell Sci. 2023 May 15;136(10):jcs26121

Insert

GFP P2A RFP_stop

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge