Showing: 21 - 40 of 62 results
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Plasmid#59855PurposeEncodes CAS9 nuclease (GFP fusion) under control of the Toxoplasma gondii SAG1 promotor. Vector also provides U6 controlled expression of a single guide RNA for CRISPR disruption of TGME49_290860.DepositorInsertCRISPR sg290860-6
UseCRISPR; ; toxoplasma gondiiTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pFloxed DHFR-TS*
Plasmid#100605PurposepUC19 with loxP-pDHFR-TS:DHFR-TS*(Pyr-R)-Stop-loxP-DHFR-TS 3' UTRDepositorInsertFloxed TgDHFR-TS*
UseToxoplasma expressionTagsExpressionMutationPyrimethamine resistancePromoterAvailabilityAcademic Institutions and Nonprofits only -
pTUB1:OsTIR1-3FLAG, SAG1:CAT
Plasmid#87258PurposeTIR1-3FLAG fusion driven by a TUB1 promoter with a CAT drug selectable marker. The TIR1 CDS from Oryza sativa was codon optimized for T. gondii expression.DepositorInsertsTIR1
CAT
UseToxoplasma expressionTags3FLAGExpressionMutationCodon optimized for Toxoplasma gondii expressionPromoterTgSAG1 361 bp and TgTUB1 2717 bpAvailabilityAcademic Institutions and Nonprofits only -
pUPRT::Floxed DHFR-TS*
Plasmid#100606PurposeTgUPRT disruption by knock in with Floxed DHFR-TS*(Pyr-R) cassette with UPRT homology flanks.DepositorInsertTgUPRT::Floxed TgDHFR-TS*
UseToxoplasma expressionTagsExpressionMutationPyrimethamine resistancePromoterpTgDHFR-TSAvailabilityAcademic Institutions and Nonprofits only -
pmCherry.LIC.HXGPRT
Plasmid#83364PurposePlasmid contains LIC cassette upstream of an in-frame copy of mCherry and HXGPRT selectable marker cassette for endogenous tagging on the gene locusDepositorInsertmCherry
UseToxoplasma gondiiTagsmCherryExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
ptdTomato.LIC.HXGPRT
Plasmid#83365PurposePlasmid contains LIC cassette upstream of an in-frame copy of tdTomato and HXGPRT selectable marker cassette for endogenous tagging on the gene locusDepositorInserttdTomato
UseToxoplasma gondiiTagstdTomatoExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCFP.HXGPRT
Plasmid#83366PurposePlasmid contains LIC cassette upstream of an in-frame copy of CFP and HXGPRT selectable marker cassette for endogenous tagging on the gene locusDepositorInsertCFP
UseToxoplasma gondiiTagsCFPExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCAT-YFP-TetR
Plasmid#59018PurposeExpresses a fusion of yellow fluorescent protein (YFP) to the N-terminus of the Escherichia coli Tn10 tet repressor (TetR) driven by the alpha tubulin promoter for use in T. gondiiDepositorInsertYFP-TetR
UseToxoplasma expressionTagsExpressionMutationPromoteralpha-tubulinAvailabilityAcademic Institutions and Nonprofits only -
pU6-DHFR
Plasmid#80329PurposeAllows for expression of an sgRNA in Toxoplasma gondii and confers resistance to pyrimethamineDepositorInsertDHFR-TSc3
UseCRISPRTagsExpressionMutationPromoterDHFR-TS 5' UTRAvailabilityAcademic Institutions and Nonprofits only -
pYFP.LIC.DHFR
Plasmid#83114PurposePlasmid contains LIC cassette upstream of an in-frame copy of YFP and DHFR-TS selectable marker cassette for endogenous tagging on the gene locus.DepositorInsertYFP
UseToxoplasma gondiiTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pYFP.LIC.HXGPRT
Plasmid#83128PurposePlasmid contains LIC cassette upstream of an in-frame copy of YFP and HXGPRT selectable marker cassette for endogenous tagging on the gene locus.DepositorInsertYFP
UseToxoplasma gondiiTagsYFPExpressionMutationPromoterEndogenous GeneAvailabilityAcademic Institutions and Nonprofits only -
pSF-TAP.LIC.HXGPRT
Plasmid#83367PurposePlasmid contains LIC cassette upstream of an in-frame copy of SF-TAP and HXGPRT selectable marker cassette for endogenous tagging on the gene locusDepositorInsertStrep-FLAG
UseToxoplasma gondiiTagsSF-TAPExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pTUB1:YFP-AID-3HA, DHFR-TS:HXGPRT
Plasmid#87260PurposeYFP-AID-3HA fusion driven by a minimal TUB1 promoter with an HXGPRT drug selectable marker. The AID CDS is Arabidopsis thaliana auxin-responsive protein IAA17.DepositorInsertsYFP-AID-3HA
HXGPRT
UseToxoplasma expressionTags3HA and AIDExpressionMutationPromoterTgDHFR-TS 463 bp and TgTUB1 378 bpAvailabilityAcademic Institutions and Nonprofits only -
ATSR
Plasmid#50052Purposeexpresses t.gondii VWA+TSR1 domains in mammalian cellsDepositorInsertMIC2-ATSR
UseTagsHISExpressionMammalianMutationS158APromoterChick beta-actin promoterAvailabilityAcademic Institutions and Nonprofits only -
proATSR
Plasmid#50053PurposeExpresses T.gondii prodomain+VWA+TSR1 domains in mammalian cellsDepositorInsertMIC2-proATSR
UseTagsHISExpressionMammalianMutationS158APromoterchick beta-actin promoterAvailabilityAcademic Institutions and Nonprofits only -
PP-mACP
Plasmid#25124PurposeBacterial expression for structure determination; may not be full ORFDepositorInsert59.m03510:C40207
UseTagsHisExpressionBacterialMutationPromoterAvailabilityIndustry, Academic Institutions, and Nonprofits -
PP-ROP2A
Plasmid#25130PurposeBacterial expression for structure determination; may not be full ORFDepositorInsert33.m01398:C43601
UseTagsHisExpressionBacterialMutationPromoterAvailabilityIndustry, Academic Institutions, and Nonprofits -
A5TSR
Plasmid#50358Purposeexpresses T.gondii MIC2(67-593) domains in mammalian cellsDepositorInsertMIC2-A5TSR
UseTagsHISExpressionMammalianMutationS158A, N357S, N463S, N470DPromoterCHICK beta-actin promoterAvailabilityAcademic Institutions and Nonprofits only -
A6TSR
Plasmid#50359Purposeexpresses T.gondii MIC2(67-660) domains in mammalian cellsDepositorInsertMIC2-A6TSR
UseTagsHISExpressionMammalianMutationS158A, N357S, N463S, N470DPromoterCHICK beta-actin promoterAvailabilityAcademic Institutions and Nonprofits only -
pLinker-BirA-3xHA-HXGPRT-loxP
Plasmid#86668Purposefor generation of BirA tagged strains using CRISPR tagging technologyDepositorInsertLinker-BirA-3xHA-HXGPRT 3'UTR
UseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 21 - 40 of 62 results