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Plasmid#155259PurposePlasmid for the aTc-inducible expression of the v5.9DepositorInsertv5.9
UseTags6xHisExpressionBacterialMutationPromotertet PAAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWUR702
Plasmid#53079PurposeInducible TtAgo expressionDepositorInsertTtAgo
UseTagsStrep-II tagExpressionBacterialMutationPromoterT7Available sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-TS2126 RnlA-Strep
Plasmid#159350PurposeExpress TS2126 RNA ligase with His6 tag and Strep tag at N and C terminal, respectivelyDepositorInsertTS2126 RNA ligase
UseTagsHis6 and StrepExpressionBacterialMutationPromoterT7Available sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
P74-26 Tail Tube Protein (gp93) in pSMT3
Plasmid#191261PurposeExpresses P74-26 gp93 with a His-SUMO tag in bacterial cellsDepositorInsertP74-26 gp93
UseTagsHis-SUMOExpressionBacterialMutationPromoterT7Available sinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
T. aq RNA Polymerase
Plasmid#215349PurposeExpress T. aq RNA Polymerase subunits in E. coli cellsDepositorInsertRNA polymerase
UseTags6x HisExpressionBacterialMutationPromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
T. aq sigmaA
Plasmid#215350PurposeExpress T. aq sigmaA transcription initiation factor in E. coli cellsDepositorInsertrpoD
UseTags6x HisExpressionBacterialMutationPromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWUR703
Plasmid#53082Purposeinducible TtAgoDM (D478A,D546A) expressionDepositorInsertTtAgo
UseTagsStrep-II tagExpressionBacterialMutationD478A D546APromoterT7Available sinceMay 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pACYC-TtCas6-4xcrRNA4.5
Plasmid#127764PurposeFor expression of T. thermophilus Csm complex in E. coli (crRNA)DepositorInsertsSynthetic CRISPR array containing four identical T. thermophilus spacers
Cas6A
UseTagsExpressionBacterialMutationCodon optimized for expression in E. coliPromoterT7Available sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOpen-TthRT
Plasmid#165566PurposeDNA polymerase that efficiently reverse-transcribes RNA in the presence of MnCl2DepositorInsertThermus thermophilus (Tth) RT
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 17, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
mV-TtCBD_656
Plasmid#105824PurposeExpresses mVenus-fusion protein of MxCBD (Thermus thermophilus CarH)DepositorInsertmV-TtCBD
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOVC1_X_TtCarH-CBD
Plasmid#131190PurposeExpresses TtCarH-CBD (Thermus termophilus) preceded by restriction sitesDepositorInsertTtCarH-CBD
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOVC1_TtCarH-CBD_X
Plasmid#131179PurposeExpresses TtCarH-CBD (Thermus termophilus) followed by restriction sitesDepositorInsertTtCarH-CBD
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-hTtAgo
Plasmid#104383Purposehuman codon optimized TtAgo; pCDNA3 vector backbone, mammalian expressionDepositorInserthTtAgo
UseTags3XFlag, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET_BGL3
Plasmid#101912PurposeExpresses a β-glucosidase gene from Rhodothermus marinus in E. coli under the control of the pET system.DepositorInsertβ-glucosidase
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7+LacOAvailable sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRD551
Plasmid#220789PurposeOverexpresses HMfC in E. coli cellsDepositorInsertHMfC
UseTagsExpressionBacterialMutationPromoterT7 promotorAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC19-A0LWB3
Plasmid#103070PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertA0LWB3(Cas9 coding gene from Acidothermus cellulolyticus (strain ATCC 43068 / 11B))
UseCRISPR; Cloning vectorTagsExpressionMutationhuman codon-optimizedPromoterAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only