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Plasmid#203659PurposeYeast vector encoding donor DNA for integration of 40nm GEMs into the HO locus.DepositorArticleInsertPfV
UseCRISPRTagsSapphireExpressionMutationPromoterAvailable sinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUBC-Pfv-Sapphire_PURO
Plasmid#203651PurposeMammalian lentiviral vector for the expression of the Sapphire-tagged 40nm GEM under the UBC promoter, with a puromycin selection marker.DepositorArticleInsertPfV
UseLentiviralTagsSapphireExpressionMutationPromoterAvailable sinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUBC-Pfv-Sapphire
Plasmid#203649PurposeMammalian lentiviral vector for the expression of the Sapphire-tagged 40nm GEM under the UBC promoter.DepositorArticleInsertPfV
UseLentiviralTagsSapphireExpressionMutationPromoterAvailable sinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
TetCMV-Pfv-Sapphire
Plasmid#203650PurposeMammalian lentiviral vector for the expression of the Sapphire-tagged 40nm GEM under the doxycycline-inducible promoter.DepositorArticleInsertPfV
UseLentiviralTagsSapphireExpressionMutationPromoterAvailable sinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-sapphireAKAR-NLS
Plasmid#118467Purposecp-T-sapphire-based single-color PKA biosensor targeted to the nucleus.DepositorInsertsapphireAKAR-NLS
UseTagsNuclear localization signal (NLS)ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyn-sapphireAKAR
Plasmid#118466Purposecp-T-sapphire-based single-color PKA biosensor targeted to the plasma membrane.DepositorInsertLyn-sapphireAKAR
UseTagsN-terminal targeting sequence from Lyn kinaseExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pINO4-NLS-Pfv-Sapphire-HO
Plasmid#203660PurposeYeast vector encoding donor DNA for integration of nuclear 40nm GEMs into the HO locus.DepositorArticleInsertPfV
UseCRISPRTagsSapphireExpressionMutationPromoterAvailable sinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-pCMV-PfV-GS-Sapphire
Plasmid#116933PurposeMammalian vector for expression of the 40nm GEMDepositorInsertPfv
UseTagsSapphireExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-Chronos-mT-Sapphire
Plasmid#149690PurposeFor packaging and expression of Chronos-mT-Sapphire in neurons via AAVDepositorInsertChronos
UseAAVTagsmT-SapphireExpressionMammalianMutationPromoterhSynAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUBC-SV40NLS-Pfv-Sapphire
Plasmid#203653PurposeMammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the UBC promoter.DepositorArticleInsertPfV
UseLentiviralTagsSapphireExpressionMutationPromoterAvailable sinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Tsapphire-StagRFP-SrcAR
Plasmid#173018PurposeLong-Stokes-shift green-red FRET-based Src activity reporter for multiplexed imaging applications.DepositorInsertTsapphire-StagRFP-SrcAR
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yETSapphire-CaUra3
Plasmid#44650DepositorInsertTurbo Sapphire
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRS306-URA3-PHIS3-AqLumSynth –Sapphire
Plasmid#116931PurposeYeast integrative vector for the exression of the 20nm GEM under the HIS3 promoterDepositorInsertlumazine synthase
UseTagsSapphireExpressionYeastMutationPromoterHIS3Available sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS305-Leu2-PINO4-PfV-GS-Sapphire
Plasmid#116930PurposeYeast integrative vector for the exression of the 40nm GEM under the INO4 promoterDepositorInsertPfv
UseTagsSapphireExpressionYeastMutationPromoterINO4Available sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS306-URA3-PHIS3-PfV-GS-Sapphire
Plasmid#116932PurposeYeast integrative vector for the exression of the 40nm GEM under the HIS3 promoterDepositorInsertPfv
UseTagsSapphireExpressionYeastMutationPromoterHIS3Available sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PfV-Sapphire-IRES-DSRed
Plasmid#116934PurposeMammalian lenti vector for expression of the 40nm GEM and DSRedDepositorInsertPfv
UseLentiviralTagsSapphireExpressionMutationPromoterCMVAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUBC-SV40NLS-Pfv-Sapphire-PURO
Plasmid#203654PurposeMammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the UBC promoter, with a puromycin selection marker.DepositorArticleInsertPfV
UseLentiviralTagsSapphireExpressionMutationPromoterAvailable sinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-Chronos-mT-Sapphire-NRN
Plasmid#149692PurposeFor packaging and expression of Chronos-mT-Sapphire featuring a neuritin (NRN) 3' UTR in neurons via AAVDepositorInsertChronos
UseAAVTagsmT-SapphireExpressionMammalianMutationPromoterhSynAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1a-tetR-T2A-tSapphire
Plasmid#176312Purposeconstruct expresses tet repressor (tetR) to reduce leakiness together with the fluorochrome t-SapphireDepositorInsertstetR
T-sapphire
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS403-HIS3-pINO4-SV40NLS-PfV-GS-Sapphire
Plasmid#203658PurposeYeast integrative vector for the expression of the Sapphire-tagged nuclear localizing 40nm GEMs under the HIS3 promoter.DepositorArticleInsertPfV
UseTagsSapphireExpressionYeastMutationPromoterAvailable sinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only