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SPSB1
Plasmid#38981PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertSPSB1 (SPSB1 Human)
UseTags6xHis-TevExpressionBacterialMutationResidues 24-233 of SPSB1 are present in this plas…PromoterAvailable sinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pKD077
Plasmid#136473PurposeSSB-mTur2 IDL fusion expression plasmidDepositorInsertSSB-mTur2 (ssb E. coli)
UseTagsmTurquoise2ExpressionBacterialMutationmTurquoise2 inserted between Phe148 and Ser149PromoterT7 promoterAvailable sinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKD072
Plasmid#136472PurposeSSB-C-term-GFP fusion expression plasmidDepositorInsertSSB-C-term-GFP (ssb E. coli)
UseTagssuperfolder GFPExpressionBacterialMutationsuperfolder GFP attached to C-terminus at Phe 178PromoterT7 promoterAvailable sinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKD068
Plasmid#136471PurposeSSB-GFP IDL fusion expression plasmidDepositorInsertSSB-GFP (ssb E. coli)
UseTagssuperfolder GFPExpressionBacterialMutationsuperfolder GFP inserted between Phe148 and Ser149PromoterT7 promoterAvailable sinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
ALARM NMR
Plasmid#90209PurposeExpresses the human La antigen for use in ALARM NMRDepositorInsertLa antigen (SSB Human)
UseTags6xHisExpressionBacterialMutationcontains amino acids 100-324PromoterT7 promoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE7-mutant (Q20A_Y23A_Y24F_F35A)
Plasmid#224123PurposeMammalian expression of SpCas9 PE7 mutant prime editor (Q20A_Y23A_Y24F_F35A)DepositorInsertPE7-mutant (Q20A_Y23A_Y24F_F35A)
UseTagsSV40 bpNLS and c-Myc NLSExpressionMammalianMutationQ20A_Y23A_Y24F_F35A in La(SSB)1-194PromoterCMVAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE7
Plasmid#214812PurposeMammalian expression of SpCas9 PE7 prime editorDepositorInsertPE7
UseTagsSV40 bpNLS and c-Myc NLSExpressionMammalianMutationPromoterCMVAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT7-PE7 for IVT
Plasmid#214813PurposeTemplate for in vitro transcription of PE7DepositorInsertPE7
UseTemplate for in vitro transcriptionTagsSV40 bpNLS and c-Myc NLSExpressionMutationPromoterT7 (inactivated)Available sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
T7_CC_PE7_IVT_Template
Plasmid#223022PurposeTemplate for in vitro transcription of PE7. For HSPC-related experiments.DepositorInsertPE7
UseTemplate for in vitro transcriptionTagsSV40 bpNLS and c-Myc NLSExpressionMutationPromoterT7Available sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pCMV-PE7-P2A-BSD
Plasmid#224122PurposeMammalian expression of SpCas9 PE7 prime editor with P2A-BSD markerDepositorInsertPE7-P2A-BSD
UseTagsP2A-BSD, SV40 bpNLS, and c-Myc NLSExpressionMammalianMutationPromoterCMVAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE7-P2A-GFP
Plasmid#222996PurposeMammalian expression of SpCas9 PE7 prime editor with P2A-EGFP markerDepositorInsertPE7-P2A-EGFP
UseTagsP2A-EGFP, SV40 bpNLS, and c-Myc NLSExpressionMammalianMutationPromoterCMVAvailable sinceAug. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
PE7_AAVS1_knockin_HDR_donor
Plasmid#223001PurposeHDR donor plasmid for PE7 knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PE7-IRES2-EGFP-WPRE-βglobinpA-right_homology_armDepositorInsertPE7
UseHdr donorTagsSV40 bpNLS and c-Myc NLSExpressionMammalianMutationPromoterEF1αAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE7-P2A-hMLH1dn
Plasmid#222997PurposeMammalian expression of SpCas9 PE7 prime editor with P2A-human MLH1dn (codon optimized)DepositorInsertPE7-P2A-hMLH1dn
UseTagsSV40 bpNLS and c-Myc NLSExpressionMammalianMutationDeletion of residues 754-756 in MLH1 as described…PromoterCMVAvailable sinceAug. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX335-NEAT1_IS_v1
Plasmid#97085PurposeEncodes an sgRNA that creates a SSB near the poly(A) site of human NEAT1_1 isoform.DepositorInsertsgRNA NEAT1_IS_v1
UseCRISPRTagsExpressionMutationPromoterU6Available sinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX335-NEAT1_IS_v2
Plasmid#97086PurposeEncodes an sgRNA that creates a SSB near the poly(A) site of human NEAT1_1 isoform.DepositorInsertsgRNA NEAT1_IS_v2
UseCRISPRTagsExpressionMutationPromoterU6Available sinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pETM33_SPSB1_SPRY
Plasmid#178487PurposeBacterial expression of human domain with His-tag and GST tagDepositorInsertSPSB1_SPRY (SPSB1 Synthetic, Human)
UseTagsHis-GSTExpressionBacterialMutationPromoterT7/LacOAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET29a-hNABP2
Plasmid#128307PurposeExpress hNABP2 (a.k.a hSSB1 or SOSSB1) in E. coli with a C-terminal His-tagDepositorInsertHuman Nucleic Acid Binding Protein 2 (hNABP2) (NABP2 Human)
UseTagsHisExpressionBacterialMutationY85C- please see depositor comments belowPromoterT7Available sinceAug. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET29a-hNABP1
Plasmid#128306PurposeExpress hNABP1 (a.k.a hSSB2 or SOSSB2) in E. coli with a C-terminal His-tagDepositorInsertHuman nucleic acid-binding protein 1 (hNABP1) (NABP1 Human)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only
