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Plasmid#134350PurposeTargeting vector for site specific integration of doxycycline inducible expression of full length human SHH cDNA into human AAVS1 locusDepositorInsertSHH (SHH Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
tet-pLKO.puro_shHuR CDS
Plasmid#110411PurposeTRCN0000276129 (Target CGAGCTCAGAGGTGATCAAAG), silence human ELAVL1 (HuR) gene, doxycycline inducible, puromycin selectionDepositorInsertELAVL1 (HuR)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterH1/TO (RNA PolIII)Available sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-TRC.mKO2_shHuR CDS
Plasmid#110426PurposeTRCN0000276129 (Target CGAGCTCAGAGGTGATCAAAG), silence human ELAVL1 (HuR) gene and express monomeric Kusabira-Orange2.DepositorInsertELAVL1 HuR
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6 (RNA Pol III)Available sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
tet-pLKO.puro_shHuR 3UTR
Plasmid#110412PurposeTRCN0000276186 (Target TTGTTAGTGTACAACTCATTT), silence human ELAVL1 (HuR) gene, doxycycline inducible, puromycin selectionDepositorInsertELAVL1 (HuR)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterH1/TO (RNA PolIII)Available sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ShhN-Ren
Plasmid#37677DepositorInsertshh (Shh Mouse)
UseTagsrenilla luciferaseExpressionMammalianMutationsoluble form (see comments)PromoterCMVAvailable sinceNov. 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
RCAS (A) shh (CT#251)
Plasmid#13991DepositorInsertshh (SHH Chicken)
UseRetroviral; Avian expressionTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2007AvailabilityAcademic Institutions and Nonprofits only -
RCAS (E) shh (CT#489)
Plasmid#13992DepositorInsertshh (SHH Chicken)
UseRetroviral; Avian expressionTagsExpressionMutationPromoterAvailable sinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-SRSF2-P95_R102delPPDSHHSR
Plasmid#116684PurposeLentiviral expression of SRSF2 P95_R102delPPDSHHSRDepositorInsertSRSF2 (SRSF2 Human)
UseLentiviralTagsExpressionMutationP95_R102delPPDSHHSRPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHTSHP-Smt-IIShhN
Plasmid#121135PurposeExpresses N-terminal SUMO tagged II-ShhN in bacteriaDepositorInsertShh (Shh Mouse)
UseTagsHIS, HRV3C site, MBP, and Smt tagExpressionBacterialMutationAmino acid sequence from 26-195, with two extra i…PromotertacAvailable sinceJan. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
MSCV-PM mir shHoxB9 b
Plasmid#27020DepositorInsertmir HoxB9 3b
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
AAV-RedO-shHsp90ab1(#c)
Plasmid#206359PurposeAAV plasmid expressing HSP90AB1 shRNA in photoreceptorsDepositorInsertshRNA of Hsp90ab1 (#c)
UseAAVTagsExpressionMutationPromoterhuman red opsinAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-RedO-shHsp90ab1 (#a)
Plasmid#206357PurposeAAV plasmid expressing HSP90AB1 shRNA in photoreceptorsDepositorInsertshRNA #a of Hsp90ab1
UseAAVTagsExpressionMutationPromoterhuman red opsinAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHTSHP-ShhN-Cys
Plasmid#121136PurposeExpresses ShhN with a C-terminal cysteine residues in bacteriaDepositorInsertshh (Shh Mouse)
UseTagsHIS, HRV3C site, and MBPExpressionBacterialMutationAmino acid sequence from 26-191, with an addition…PromotertacAvailable sinceMarch 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
MSCV-PM mir shHoxB9 a
Plasmid#27021DepositorInsertmir HoxB9 2A
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
PCR4-Shh::lacZ-H11
Plasmid#139098PurposeenSERT LacZ reporter vector for site-specific integration into the H11 locus (contains Shh minimal promoter)DepositorTypeEmpty backboneUseCRISPR and Mouse TargetingTagsExpressionMammalianMutationPromoterShhAvailable sinceMarch 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDonor14-ShHELIX-KanR (CJT70)
Plasmid#181793PurposepDonor for ShHELIX containing 14bp of spacing between the I-AniI site and LE/RE and encoding a kanamycin resistance gene as cargoDepositorInsertKanR, R6K origin of replication
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCCLc-U6-shHMGA2.3-PGK-dTomato
Plasmid#89606PurposeSilencing Hmga2 in human cellsDepositorInsertshRNA targeting HMGA2 (HMGA2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJuly 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCCLc-U6-shHMGA2.2-PGK-dTomato
Plasmid#89605PurposeSilencing Hmga2 in human cellsDepositorInsertshRNA targeting HMGA2 (HMGA2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET19b-rShHTL7 cPGFP(E167)
Plasmid#166004PurposeExpression of coding sequence in Escherichia coliDepositorInsertrShHTL7 cPGFP(E167)
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET19b-rShHTL7 cPGFP(D165)
Plasmid#166003PurposeExpression of coding sequence in Escherichia coliDepositorInsertrShHTL7 cPGFP(D165)
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only