Addgene

Showing: 21 - 40 of 234 results

Results per page:

Sort results by:

PGK1 gRNA (BRDN0001146969)

Plasmid

#77982

Purpose

3rd generation lentiviral gRNA plasmid targeting human PGK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PGK1 (PGK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
p415PGK1-AtMAX1(pYL759)

Plasmid

#178289

Purpose

Expresses MAX1 from A. thaliana (AtMAX1) in Saccharomyces cerevisiae. Centromeric LEU, PPGK1-AtMAX1-Tpho5

Depositor

Yanran Li

Article

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

AtMAX1

Use

Tags

Expression

Yeast

Mutation

Promoter

PGK1

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
p416PGK1-OsCYP711A2(pYL770)

Plasmid

#178301

Purpose

Expresses OsCYP711A2 from O. sativa in Saccharomyces cerevisiae. Centromeric URA, PPGK1-OsCYP711A2-Tpho5

Depositor

Yanran Li

Article

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

OsCYP711A2

Use

Tags

Expression

Yeast

Mutation

Promoter

PGK1

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
plenti hSyn PGK1

Plasmid

#220919

Purpose

Neuronal specific expression of PGK1

Depositor

Timothy Ryan

Article

Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

Insert

PGK1 (PGK1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL4.22-PGK1-HRE::dLUC

Plasmid

#128095

Purpose

Hypoxia response element real-time luciferase reporter

Depositor

Chi Van Dang

Article

Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

Insert

Hypoxia response element (x3) from PGK1 promoter (PGK1 Human)

Use

Luciferase

Tags

drives expression of destabilized luciferase (2CP…

Expression

Mammalian

Mutation

Promoter

inserted PGK1 hypoxia response elements (HRE) (x3)

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
AAVS1_Puro_hPGK1_hGEM(1-110)_mNG_hSLBP(18-108)

Plasmid

#176932

Purpose

Donor for targeted integration of S-phase specific probe (FUCCI-S) to the AAVS1 safe harbor locus

Depositor

Yannick Doyon

Insert

hGEM(1-110)_mNG_hSLBP(18-108)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

PGK1

Available since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pFRT/TO/HIS/FLAG/HA-PGK1

Plasmid

#38071

Depositor

Markus Landthaler

Article

Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

Insert

PGK1 phosphoglycerate kinase 1 (PGK1 Human)

Use

Tags

HIS/FLAG/HA

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 17, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pPpT4- PGK1prom-TIR1-FLAG

Plasmid

#189724

Purpose

Enables expression of OsTIR1 from the PGK1 promoter, used to implement the AID system in Komagataella phaffii (original name pAEA454)

Depositor

Anita Emmerstorfer-Augustin

Article

Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

Insert

OsTIR1

Use

Tags

Expression

Yeast

Mutation

Promoter

PGK1 promoter

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV hSyn hPGK1-mRuby

Plasmid

#220911

Purpose

Neuronal specific expression of the fusion construct PGK1-mRuby

Depositor

Timothy Ryan

Article

Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

Insert

PGK1-mRuby (PGK1 Human)

Use

AAV

Tags

mRuby

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pVH233-Tier1-PmPGK1-eYFP

Plasmid

#169522

Purpose

Tier-1 vector encoding PmPGK1-driven eYFP expression (PmPGK1-eYFP-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PPGK-driven enhanced yellow fluorescent protein

Use

Tags

Expression

Mammalian

Mutation

Promoter

PPGK

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pVH231-Tier1-PmPGK1-mTagBFP2

Plasmid

#169520

Purpose

Tier-1 vector encoding PmPGK1-driven mTagBFP2 expression (PmPGK1-mTagBFP2-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PPGK-driven monomeric Tag blue fluorescent protein 2

Use

Tags

Expression

Mammalian

Mutation

Promoter

PPGK

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
plenti hSyn PGK1-HALO

Plasmid

#220910

Purpose

Neuronal specific expression of the fusion construct PGK1-HALO

Depositor

Timothy Ryan

Article

Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

Insert

PGK1-HALO (PGK1 Human)

Use

Lentiviral

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pVH234-Tier1-PmPGK1-tTA

Plasmid

#169574

Purpose

Tier-1 vector encoding PmPGK1-driven tTA expression (PmPGK1-tTA-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

tetracycline-controlled transactivator

Use

Tags

Expression

Mammalian

Mutation

Promoter

PPGK

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pVH232-Tier1-PmPGK1-mRuby2

Plasmid

#169523

Purpose

Tier-1 vector encoding PmPGK1-driven mRuby2 expression (PmPGK1-mRuby2-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PPGK-driven monomeric red fluorescent protein mRuby2

Use

Tags

Expression

Mammalian

Mutation

Promoter

PPGK

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB1975 (pUC19-PTEF1-PPGK1)

Plasmid

#73296

Purpose

Example plasmid containing a bidirectional promoter

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

pTEF1-pPGK1

Use

Synthetic Biology

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pVH235-Tier1-PmPGK1-rtTA

Plasmid

#169578

Purpose

Tier-1 vector encoding PmPGK1-driven rtTA expression (PmPGK1-rtTA-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

reverse tetracycline-controlled transactivator

Use

Tags

Expression

Mammalian

Mutation

Promoter

PPGK

Available since

July 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pVH230-2-Tier1-PmPGK1-YPet

Plasmid

#169521

Purpose

Tier-1 vector encoding PmPGK1-driven YPet expression (PmPGK1-YPet-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PPGK-driven yellow fluorescent protein Ypet

Use

Tags

Expression

Mammalian

Mutation

Promoter

PPGK

Available since

June 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pVH237-2-Tier1-PmPGK1-TtgA

Plasmid

#169584

Purpose

Tier-1 vector encoding PmPGK1-driven TtgA expression (PmPGK1-TtgA-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

Regulatory protein TtgR fused to a VP16 domain

Use

Tags

Expression

Mammalian

Mutation

Promoter

PPGK

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pVH608-Tier1-PmPGK1-Fluc

Plasmid

#169543

Purpose

Tier-1 vector encoding PmPGK1-driven Fluc (PmPGK1-Fluc-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PPGK-driven Firefly Luciferase

Use

Tags

Expression

Mammalian

Mutation

Promoter

PPGK

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDSM-de-Ppgk1-PYC-Ttpi1

Plasmid

#127735

Purpose

Yeast pathway position 5. PYC transcription unit with the PGK1 promoter and TPI1 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

pyruvate carboxylase (PYC1 Synthetic, Budding Yeast)

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Ppgk1

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge