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Plasmid#22905DepositorInsertSYFP2-SCFP3A
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pSYC-437
Plasmid#163829PurposeFoxj1a:GCaMP6s. Fluorescent reporter for calcium signaling (GCaMP6s) driven by zebrafish foxj1a promoter.DepositorInsertsFoxj1a Promoter
GCaMP6s
UseMultisite gateway destination vectorTagsExpressionMutationPromoterAvailable sinceFeb. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSYC-190
Plasmid#178962PurposeExpresses human GFAP (R79C) mutant fused to 3xFLAG tag in mammalian cellsDepositorInsertGlial fibrillary acidic protein (GFAP Human)
UseTags3xFLAGExpressionMammalianMutationR79CPromoterCMV IE94 PromoterAvailable sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSYC-187
Plasmid#74794PurposepCS4+-P2A-mCherry-CAAXDepositorInsertP2A-mCherry-CAAX
UseTagsExpressionMutationPromoterAvailable sinceJune 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S_Psyn-sgRNA500
Plasmid#149640Purposeparental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloningDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30, PsynAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSY14-ptb
Plasmid#90490PurposepSY8 derivative with sceC gene under the control of ptb promoter used for I-SceI-mediated scarless gene modification via allelic exchange in ClostridiumDepositorInsertsceC
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSYC-181
Plasmid#31566DepositorInsertpCS4+-P2A
UseTagsExpressionMutationPromoterAvailable sinceJune 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSYC-102
Plasmid#74790PurposepCS4+-NLS-EGFP-T2A-mCherry-CAAXDepositorInsertNLS-EGFP-T2A-mCherry-CAAX
UseTagsExpressionMutationPromoterAvailable sinceJune 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSYC-104
Plasmid#74792PurposepCS4+-NLS-EGFP-F2A-mCherry-CAAXDepositorInsertNLS-EGFP-F2A-mCherry-CAAX
UseTagsExpressionMutationPromoterAvailable sinceJune 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSYC-103
Plasmid#74791PurposepCS4+-NLS-EGFP-E2A-mCherry-CAAXDepositorInsertNLS-EGFP-E2A-mCherry-CAAX
UseTagsExpressionMutationPromoterAvailable sinceJune 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S_Psyn-sgRNAflaB
Plasmid#149642Purposeall-in-one CRISPRi vector for targeting B. burgdorferi flaBDepositorInsertdCas9, lacI, sgRNAflaB
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30, PsynAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S_Psyn-sgRNAmreB
Plasmid#149653Purposeall-in-one CRISPRi vector for targeting B. burgdorferi mreBDepositorInsertdCas9, lacI, sgRNAmreB
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30, PsynAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKFSS1_Psyn-sgRNA500
Plasmid#149557PurposegRNA expression in B. burgdorferi, parental vectorDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterPsyn and pSynAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBSV2_Psyn-mCherryBb
Plasmid#149636PurposePsyn-mCherry promoter strength reporter for use in B. burgdorferiDepositorInsertPsyn-mCherryBb
UseTagsmCherryExpressionBacterialMutationPromoterPsynAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
PSY1R C3_pECIA2
Plasmid#115079PurposeBait vector PSY1R C3_pECIA2 should be used with prey vector PSY1R C3_pECIA14.DepositorInsertAT1G72300 (PSY1R Mustard Weed)
UseTagsFc, V5 and hexahistidine tagsExpressionInsectMutationPromoterMetallothionein (Copper-Inducible)Available sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
PSY1R C3_pECIA14
Plasmid#114879PurposePrey vector PSY1R C3_pECIA14 should be used with bait vector PSY1R C3_pECIA2.DepositorInsertAT1G72300 (PSY1R Mustard Weed)
UseTagsExpressionInsectMutationPromoterMetallothionein (Copper-Inducible)Available sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pETmsGFPSyt1(80-142)
Plasmid#209012PurposeRecombinant protein expression of truncated syt1 tagged with GFPDepositorInsertsyt1 (Syt1 Rat)
UseTags6xHis-msfGFPExpressionBacterialMutationaa 80-142 onlyPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSYC-204
Plasmid#178964PurposeHuman GFAP (R79C) mutant fused to 3xFLAG tag and EGFP driven by zebrafish gfap promoter for Tol2 mediated recombinationDepositorInsertsUseTol2 mediated recombinationTags3xFLAGExpressionMutationR79CPromoterZebrafish gfap promoterAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSYC-201
Plasmid#178963PurposeHuman GFAP fused to 3xFLAG tag and EGFP driven by zebrafish gfap promoter for Tol2 mediated recombinationDepositorInsertsUseTol2 mediated recombinationTags3xFLAGExpressionMutationPromoterzebrafish gfap promoterAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
LjUBQ:MtPSY6:T35s
Plasmid#164769PurposeOverexpression of Peptide coding gene (Binary vector)DepositorInsertMedtr4g108700.1
UseTagsTerminator 35SExpressionPlantMutationPromoterLotus japonicus UbiquitinAvailable sinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only