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Plasmid#68375PurposeDoyon lab Tandem-Affinity Purification Following (i) Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus and (ii) Endogenous TAP Tagging.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPR and TALEN; ZfnTags3xFLAG_Twin_StrepExpressionMammalianMutationPromoterhuman PGK1Available sinceOct. 13, 2015AvailabilityAcademic Institutions and Nonprofits only
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pHR_Gal4UAS_IRES_mC_pGK_tBFP
Plasmid#79123PurposeLentiviral vector for inducible expression of genes with Gal4DBDVP64 synNotch ReceptorsDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHR_Gal4UAS_tBFP_PGK_mCitrine
Plasmid#188386PurposeLentiviral vector - tBFP reporter for Gal4-based SNIPRs with a constitutive mCitrine;DepositorInsertGal4UAS_tBFP_PGK_mCitrine
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti.PGK.chFP.W
Plasmid#51008PurposeLentivector that expresses chFP from internal human PGK promoterDepositorInsertmChFP
UseLentiviralTagsExpressionMammalianMutationPromoterhuman PGKAvailable sinceFeb. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
EF.PGK.GFP
Plasmid#17618DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRRlsinPGK_CREGFP_WPRE
Plasmid#91789PurposeConstitutive expression of creGFP fusion using PGK promoter in a lentiviral backbone.DepositorInsertCre-GFP fusion protein
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterPGKAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAVS1_Puro_hPGK1_EGFP_Donor
Plasmid#178088PurposeHDR donor plasmid to introduce the EGFP cassette to AAVS1 using AAVS1 T2 sgRNA.DepositorInsertEGFP
UseCRISPRTagsExpressionMammalianMutationPromoterHuman PGK1Available sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRRlsinPGK_H2BGFP_WPRE
Plasmid#91788PurposeConstitutive expression of H2BGFP fusion using PGK promoter in a lentiviral backbone.DepositorInsertHuman histone H2B (H2BC11 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterPGKAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti_PGK_Prkaa1
Plasmid#204356PurposeLentiviral plasmid, Expresses Prkaa1 under the control of the PGK promoterDepositorInsertAMP-activated protein kinase alpha 1 (Prkaa1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_antiCD19_synNotch_TetRVP64
Plasmid#79126PurposeLentiviral vector for constitutive expression of the anti-CD19 scFv synNotch TetRVP64 Receptor (PGK promoter)DepositorInsertantiCD19_synNotch_TetRVP64
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_LaG16_2_synNotch_Gal4VP64
Plasmid#85421PurposeLentiviral vector for expression of LaG16_2 GFP nanobody Gal4VP64 synthetic NotchDepositorInsertLaG16_2 GFP nanobody synNotch Gal4VP64
UseLentiviralTagsExpressionMutationPromoterPGKAvailable sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK
Plasmid#79120PurposeLentiviral vector for constitutive expression of genes (PGK promoter)DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
PGKneobpA
Plasmid#13442DepositorInsertPGKneobpA
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
PGKneotpAlox2
Plasmid#13444DepositorInsertPGKneotpAlox2
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
SIN40C.TRE.Luciferase.IRES.dTomato.PGK.sfGFP.P2A.Tet3G
Plasmid#169317PurposeDoxycycline-inducible expression of Luciferase cDNA with dTomato as reporter fluorescent proteinDepositorInsertLuciferase
UseLentiviralTagsIRES-dTomatoExpressionMutationPromoterTREAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAVS1_Puro_hPGK1_EBFP_Donor
Plasmid#178089PurposeHDR donor plasmid to introduce the EBFP (T65S/F145Y) cassette to AAVS1 using AAVS1 T2 sgRNA.DepositorInsertEBFP (T65S/F145Y)
UseCRISPRTagsExpressionMammalianMutationPromoterHuman PGK1Available sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV.PGK.GFP.miR9T
Plasmid#115973PurposemiR-9 regulated GFP expressionDepositorInsertGFPmiR9T
UseLentiviralTagsGFPExpressionMutationPromoterPGKAvailable sinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR_Gal4UAS_Tbet_T2A_GFP_PGK_mCherry
Plasmid#85431PurposeLentiviral vector for Gal4 inducible Tbet T2A GFP and constitutive mCherry expressionDepositorInserthuman Tbet
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPGKFLPobpA
Plasmid#13793PurposeExpression of mouse codon-optimized FLP recombinase with bovine growth hormone polyadenylation (bpA) sequence.DepositorInsertFLPo
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pPGKPhiC31obpA
Plasmid#13795PurposeExpression of mouse codon-optimized PhiC31 recombinase with bovine growth hormone polyadenylation (bpA) sequence.DepositorInsertPhiC31o
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2007AvailabilityAcademic Institutions and Nonprofits only