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Plasmid#37132DepositorInsertLSSmOrange-DEVD-mKate2
UseTagsExpressionMammalianMutationA44V/F84L/W145M/I165D/M167L/G202D compared to mOr…PromoterCMVAvailable sinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-ChrimsonR-LSSmOrange
Plasmid#149691PurposeFor packaging and expression of ChrimsonR-LSSmOrange in neurons via AAVDepositorInsertChrimsonR
UseAAVTagsLSSmOrangeExpressionMammalianMutationPromoterhSynAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-ChrimsonR-LSSmOrange-NRN
Plasmid#149693PurposeFor packaging and expression of ChrimsonR-LSSmOrange featuring a neuritin (NRN) 3' UTR in neurons via AAVDepositorInsertChrimsonR
UseAAVTagsLSSmOrangeExpressionMammalianMutationPromoterhSynAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMSCVII-LO
Plasmid#162754PurposeRetroviral (MSCV) expression of LSSmOrange fluorescent proteinDepositorInsertLSSmOrange
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pMIII-Neo-LO
Plasmid#153428PurposeRetroviral (MSCV) expression of LSSmOrange fluorescent proteinDepositorInsertLSSmOrange
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET15b-sfGO-Matryoshka-LE-LP
Plasmid#100018PurposeBacterial expression of fluorescent protein casette - cpsfGFP with nested LSSmOrange and LE N-term and LP C-term amino acid linkersDepositorInsertcpsfGFP-LSSmOrange
UseTags6x HIS tagExpressionBacterialMutationLeu and Glu N-term linker and Leu and Pro C-term …PromoterT7Available sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET15b-eGO-Matryoshka-LE-LP
Plasmid#100017PurposeBacterial expression of fluorescent protein casette - cpEGFP with nested LSSmOrange and LE N-term and LP C-term amino acid linkersDepositorInsertcpEGFP-LSSmOrange
UseTags6x HIS tagExpressionBacterialMutationLeu and Glu N-term linker and Leu and Pro C-term …PromoterT7Available sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSET a sfMatryoshCaMP6s-T78H
Plasmid#100021PurposeBacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference LSSmOrange nested within the reporting superfolder cpGFP-T78HDepositorInsertsfMatryoshCaMP6s
UseTags6x HIS tag and Xpress_EK tagExpressionBacterialMutationcpEGFP replaced with superfolder cpGFP and T78H m…PromoterT7Available sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
AKARdual
Plasmid#99869PurposeMeasure PKA activity by conformational change after PKA substrate phosphorylation and recognition of this phosphorylated substrateDepositorInsertAKAR4
UseTagsLSSmOrange and mKate2ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET19b-rDAD2 cPGFP(N180)
Plasmid#166001PurposeExpression of coding sequence in Escherichia coliDepositorInsertrDAD2 cPGFP(N180)
UseTagsExpressionBacterialMutationLSSmOrange- V49A (please see depositor comments)PromoterT7 promoterAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV:Flag-OgNLuc
Plasmid#208851PurposeExpresses FLAG epitope-tagged OgNLuc "Orange" BRET reporter in mammalian cells.DepositorInsertOgNLuc LumiFluor
UseLentiviralTags1xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGPTVII-Bar-U-MatryoshCaMP6s
Plasmid#100024PurposePlant expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.DepositorInsertMatryoshCaMP6s
UseTagsExpressionPlantMutationPromoterAtUBQ10Available sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2-DEST-MatryoshCaMP6s
Plasmid#100025PurposeMammalian expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.DepositorInsertMatryoshCaMP6s
UseTagsV5 Epitope TagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSET a MatryoshCaMP6s
Plasmid#100019PurposeBacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.DepositorInsertMatryoshCaMP6s
UseTags6x HIS tag and Xpress_EK tagExpressionBacterialMutationPromoterT7Available sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSET a sfMatryoshCaMP6s
Plasmid#100020PurposeBacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting superfolder cpGFP.DepositorInsertsfMatryoshCaMP6s
UseTags6x HIS tag and Xpress_EK tagExpressionBacterialMutationcpEGFP replaced with superfolder cpGFPPromoterT7Available sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDRF'- AmTryoshka1;3-LS-F138I
Plasmid#100922PurposeYeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP.DepositorInsertAmTryoshka1;3-LS-F138I (AMT1;3 Mustard Weed)
UseTagsExpressionYeastMutationF138IPromoterPMAAvailable sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDRF'- AmTryoshka1;3-LS-L255I
Plasmid#100964PurposeYeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP.DepositorInsertAmTryoshka1;3-LS-L255I (AMT1;3 Mustard Weed)
UseTagsExpressionYeastMutationL255IPromoterPMAAvailable sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDRF'- AmTryoshka1;3-GS-F138I
Plasmid#100965PurposeYeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP.DepositorInsertAmTryoshka1;3-GS-F138I (AMT1;3 Mustard Weed)
UseTagsExpressionYeastMutationF138IPromoterPMAAvailable sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDRF'- AmTryoshka1;3-GS-L255I
Plasmid#100966PurposeYeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP.DepositorInsertAmTryoshka1;3-GS-L255I (AMT1;3 Mustard Weed)
UseTagsExpressionYeastMutationL255IPromoterPMAAvailable sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only