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Plasmid#135970PurposeExpresses SpCas9, and cloning backbone for sgRNADepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only
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pCDH_blast_MCS_Nard_GFP_LAMIN
Plasmid#167340PurposeExpresses GFP fused to lamin A (LA) in mammalian cellsDepositorInsertLamin A (LMNA Human)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
tetO.Nfia.Blast
Plasmid#117270PurposeExpresses Nfia and blasticidin resistance gene under control of tetO sequence.DepositorInsertNfia (Nfia Mouse)
UseLentiviralTagsExpressionMammalianMutationPromotertet-onAvailable sinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGreenFire_blastocidin
Plasmid#174103PurposepGreenFire MPRA plasmid for cloningDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Blast
Plasmid#111887PurposeLentiviral expression of cDNA with blasticidin resistance geneDepositorTypeEmpty backboneUseLentiviralTagsFLAGExpressionMammalianMutationPromoterEFS promoterAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
BPK1520_blastR
Plasmid#175289PurposeHuman expression plasmid for SpCas9 sgRNA with blasticidin co-selectionDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterCMV/U6Available sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHCMV_LASV_GPC
Plasmid#207287PurposeMammalian expression of Lassa virus GPC proteinDepositorInsertLASV_GPC
UseTagsExpressionMammalianMutationmammalian codon optimizationPromoterCMV promoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDual_dsCas9_Blast_hs_NC_chr1
Plasmid#214690PurposeLentiviral expression vector for an inducible Cas9-P2A-Blasticidin resistance casette with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)DepositorInsertdgRNA_Chr1
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDual_dsCas9_Blast_hs_TP73
Plasmid#214691PurposeLentiviral expression vector for an inducible Cas9-P2A-Blasticidin resistance casette with two sgRNA sequences against human TP73DepositorInsertdgRNA_TP73 (TP73 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
Butelase AY
Plasmid#221847PurposeExpresses Butelase AY with Val237Ala and Thr238Tyr mutationsDepositorInsertButelase 1 ligase
UseTagsExpressionBacterialMutationVal237Ala, Thr238TyrPromoterAvailable sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_LASP1_LIM
Plasmid#109780PurposeProtein expression and purification of LASP1_LIMDepositorInsertLASP1_LIM (LASP1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDH_blast_MCS_Nard_GFP_D50
Plasmid#167339PurposeExpresses GFP fused to progerin (d50) in mammalian cellsDepositorInsertProgerin
UseLentiviralTagsGFPExpressionMammalianMutation50AA deletion creating Progerin instead of mature…PromoterCMVAvailable sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_plasmid_donor_RD
Plasmid#86551PurposeHomology-directed repair (HDR) donor plasmid with homology arms to ATP1A1 to introduce the Q118R and N129D (RD) mutations conferring cellular resistance to ouabainDepositorInsertATP1A1 (ATP1A1 Human)
UseCRISPR and TALEN; Co-selection via hdr using ouab…TagsExpressionMammalianMutationQ118R, N129DPromoterAvailable sinceMarch 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_m4_Blasto
Plasmid#167864PurposePiggyBac compatible plasmid expressing dCas9DepositorInsertdCas9
UseCRISPRTagsExpressionMutationD10A, D839A, H840A, N863APromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_Blasto
Plasmid#167856PurposePiggyBac compatible plasmid expressing spCas9DepositorInsertCas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti_P2A_Blast
Plasmid#211365PurposeLentiviral expression vector for an insert of interest linked to a P2A-T2A blasticidin sequence. Produces virus very efficiently. Can also be used for regular transfectionsDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
ptetmCherry_prey_plasmid-2
Plasmid#34617DepositorInsertmCherry
UseTagsExpressionBacterialMutationPromoterptetAvailable sinceJuly 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
FFiBlast p5
Plasmid#68470PurposeInverted and floxed blasticidin resistanceDepositorInsertBlasticidin Resistance
UseCre/LoxTagsExpressionMammalianMutationInvertedPromoterLTRAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAK_Tol2_TRE_Blast
Plasmid#130261PurposeTol2 doxycycline inducible expression vectorDepositorTypeEmpty backboneUseTol2 transposon, doxycycline inducibleTagsExpressionMammalianMutationPromoterTRE PromoterAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBSVSG221KOblast
Plasmid#59733PurposeFor replacement of VSG221 with a blasticidin resistence gene in the VSG221 expression site in T.brucei.DepositorInsertVSG221 knockout construct
UseFor homologous recombination into vsg221 expressi…TagsExpressionMutationPromoterAvailable sinceJuly 15, 2015AvailabilityAcademic Institutions and Nonprofits only