Showing: 21 - 36 of 36 results
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Plasmid#209443PurposeSB-transposable plasmid expressing a mutant histone H3.3-K27M in tandem with Katushka (RFP)DepositorInsertH3.3-K27M
UseTagsExpressionMammalianMutationK27MPromoterAvailabilityAcademic Institutions and Nonprofits only -
pKT-IDH1(R132H)-IRES-Katushka
Plasmid#124257PurposeExpresses IDH1 with a R132H mutation and katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.DepositorInsertIsocitrate dehydrogenase 1 (R132H) (IDH1 Human)
UseTagsExpressionMammalianMutationArginine 132 is mutated to Histidine. This mutati…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pKT-H3.3-G34R-IRES-katushka
Plasmid#209428PurposeSB-transposable plasmid expressing the mutant histone H3.3-G34R with RFPDepositorInsertH3.3-G34R
UseTagsExpressionMammalianMutationG34RPromoterAvailabilityAcademic Institutions and Nonprofits only -
pTransgenesis p3 sv40 minimal promoter:: Katushka RFP
Plasmid#131731PurposeTransposon based transgenesisDepositorInsertSV40MinPromoter
UseXenopus expressionTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Transposon-for RMCE_Sleeping Beauty_LIR-CAG-Frt-red(katushka)-P2A-Puro-F3-PA-RIR
Plasmid#67275PurposeFor sleeping beauty transposition and establishment of an recombinase mediated cassette exchange docking site. To make RMCE in cell line instead of flp in cell linesDepositorInsertpuromycin resistant gene
UseTagslinked to red flouresent protein gene (katushka)ExpressionMammalianMutationPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
pKT-mIDH1(R132H)-Myc-FLAG-IRES-Kat
Plasmid#133779PurposeExpresses IDH1 with a R132H mutation and has Myc and FLAG tags. IDH1(R132H) also has a katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.DepositorInsertIsocitrate dehydrogenase 1 (IDH1 Human)
UseTagsMyc and FLAGExpressionMammalianMutationArginine is mutated to histidine at amino acid po…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pNK141j2
Plasmid#156383PurposeExpresses orthogonal RBS Katushka-9-5 reporterDepositorInsertKatushka-9-5
UseSynthetic BiologyTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pdx1-mTQ2-P2A-FlpO-PA
Plasmid#67279PurposePancreas specific expression of the FlpO recombinase controlled by the pdx1 promoter . FlpO is linked to mTQ2 -a blue fluorescent protein. The gene cassette is in a SB transposon contextDepositorInsertsmTQ2=mturquoise2 blue fluorescent gene
FlpO recombinase
UseTagslinked to FlpO through an P2A ribosomal skipping …ExpressionMammalianMutationPromoterpdxAvailabilityAcademic Institutions and Nonprofits only -
LIR-wtloxP-TRE-CAG-Frt-red-puro-3xPA-Frt-GFP(mVenus)-KrasG12D-cMyc-SV40LT-IRES-KRABoff-PA-TRE-loxP257-RIR
Plasmid#67277Purposeinducbile color change and cancer regulation: FlpO recombinase dependent expression of KrasG12D cMyc and SV40 large T antigen with doxycycline inducible downregulation through tetKRAB system.DepositorInsertsfirst casette contain a red fluorescent protein (katushka) linked to puromycin resitance gene through an E2A site
second cassette contains mVENUS-kras-cmyc-SV40LT-KRABoff
UseTagsmTQ2 blue fluorescent and red flourescent gene ka…ExpressionMammalianMutationPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
pEB2-2ndVal Kat9-5
Plasmid#104031PurposePlasmid encoding 2ndVal Katushka9-5DepositorInsert2ndVal Katushka9-5
UseLow copyTagsExpressionBacterialMutationKatushka9-5 with valine inserted in 2nd positionPromoterproCAvailabilityAcademic Institutions and Nonprofits only -
pPB_Cdc42-TomKat-sensor-CAAX
Plasmid#172473PurposePiggybac vector for mammalian expression of TomKat (tdTomato-tdKatushka) FRET sensor for Cdc42 activity anchored to the membrane with the polybasic and CAAX motif from Kras.DepositorInserttdKatushka2-PBD(Pak1)-EVlinker-Cdc42-tdTomato-CAAX
UsePiggybac transposonTagspolybasic and CAAX motif from Kras, tdKatushka2, …ExpressionMammalianMutationPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
pFLxTd5
Plasmid#167519PurposeBroad-Host range expression vector, RSF1010 origin, LuxR activator, PLuxB promoter, tdKatushka2, Gentamicin Resistance Marker.DepositorInsertLuxR-tdKatushka2
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLuxBAvailabilityAcademic Institutions and Nonprofits only -
pFVTd5
Plasmid#167520PurposeBroad-Host range expression vector, RSF1010 origin, VanRAM repressor, PVanCC promoter, tdKatushka2, Gentamicin Resistance MarkerDepositorInsertVanRAM-tdKatushka2
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPVanCCAvailabilityAcademic Institutions and Nonprofits only -
pcDNA4_ChR2opt_mKATE
Plasmid#67958Purposeoptical depolarization of mammalian cellsDepositorInsertChannelrhodopsin 2 opt
UseTagsmKatushkaExpressionMammalianMutationsynthetically produced for mammalian optimized co…PromoterCMV -TetONAvailabilityAcademic Institutions and Nonprofits only -
pTEC22
Plasmid#30181DepositorInsertMycobacterium Strong Promoter (MSP)
UseMycobacteria expressionTagstdKatuschka2ExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pDC-UAS-nBitbow1.0(miniW)
Plasmid#138329PurposeDrosophila attB-targeting plasmid that contains UAS driving nBitbow1.0 cassette. Expresses combinations of nuclear mAmetrine, mTFP, mNeonGree, mKusabira-Orange2, and mKate2 upon Flp recombination.DepositorInsertsmAmetrine
tdKatushka2
mNeonGreen
mTFP1
mKusabira-Orange2
UseTagshuman H2BExpressionInsectMutationPromoter10xUASAvailabilityAcademic Institutions and Nonprofits only
Showing: 21 - 36 of 36 results