Showing: 21 - 40 of 4528 results
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Plasmid#169665PurposeTier-3 vector for lentiviral stable integration of the rtTA and puromyine - Ypet selection cassette in inverted direction with polyA (PRPBSA-YPet-p2a-PuroR::PPGK-rtTA-pA::A3-pA)DepositorInsertPPGK-driven rtTA expression and PRPBSA-driven YPet and PuroR expression
UseTagsExpressionMammalianMutationPromoterPhCMV / PPGK / RPBSAAvailabilityAcademic Institutions and Nonprofits only -
pCAG-lox-inv[Talpha1-HA-Dre]-lox-mOrange2-NEDD1-gTBD
Plasmid#196894PurposeNeuron-specific expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to mOrange aimed to displace endogenous gamma-TuRC from the centrosome. Used in combination with Talpha1-iCre-pA plasmidsDepositorInsertN-gTBD-mOrange2 (NEDD1 Human)
UseCre/Lox; Dre/roxTagsExpressionMammalianMutationPromoterQuimeric CAGAvailabilityAcademic Institutions and Nonprofits only -
pCAG-lox-inv[Talpha1-iCre-pA]-lox-2xHA-128-425-Akap9
Plasmid#196897PurposeNeuron-specific expression of fragment 128-425 from A-Kinase Anchoring Protein 9 (Akap9) fused to 2xHA-tags.DepositorInsert2xHA-Akap9(128-425) (Akap9 Rat)
UseCre/LoxTagsExpressionMammalianMutationPromoterQuimeric CAGAvailabilityAcademic Institutions and Nonprofits only -
pSPIKE-E
Plasmid#101173Purposechimeric spike resembling a fragment of eukaryotic 18S rRNA to be used in metagenomicsDepositorInsertchimeric 18S rRNA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-neo-cterminal-3HA
Plasmid#102643PurposeTo generate three copies of the HA epitope tag on cDNAs.DepositorTypeEmpty backboneUseTags3x HA (YPYDVPDYA)ExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-neo-Strep_Flag_Nterm
Plasmid#102644PurposeEpitope tag n-terminus with SBP-Flag moietyDepositorTypeEmpty backboneUseTagsSBP-Flag, MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREPG…ExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-neo-Strep_Flag_Cterm
Plasmid#102645PurposeEpitope tag c-terminus with SBP-Flag moietyDepositorTypeEmpty backboneUseTagsSBP-Flag, MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREPG…ExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
E-cadherin-GFP
Plasmid#28009DepositorInsertE-cadherin (CDH1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HCMVgH (TB40/E)
Plasmid#136446PurposeMammalian expression plasmid for HCMV glycoprotein H (TB40/E strain), full-lengthDepositorInsertglycoprotein H (UL75 Human betaherpesvirus 5)
UseTagsExpressionMammalianMutationCodon-optimized for human cell expression.PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mNRDc-E>A-V5
Plasmid#51298Purposeexpression of the inactive mutant of mouse NRDc in mammalian cellsDepositorInsertNRDc E247A (Nrd1 Mouse)
UseTagsHIS and V5ExpressionMammalianMutationE247A, enzymatic inactive mutantPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCR3-FLAG-Gephyrin E
Plasmid#68819PurposeFlag tagged mammalian expression of E domainDepositorInsertGephyrin (Gphn Rat)
UseTags3xFLAGExpressionMammalianMutationinternal EcoRI site has silent mutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
p172 Dync1i2.E (Ex 1b)
Plasmid#26442DepositorInsertcytoplasmic dynein 1 intermediate chain 2 isoform E (exon 1b) (Dync1i2 Mouse)
UseSubcloning and sequencingTagsExpressionMutationN/APromoterAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HCMVgL-FLAG (TB40/E)
Plasmid#136449PurposeMammalian expression plasmid for HCMV glycoprotein L (TB40/E strain) with FLAG tagDepositorInsertglycoprotein L (UL115 Human betaherpesvirus 5)
UseTagsShort SGGSG linker and FLAG tagExpressionMammalianMutationCodon-optimized for human cell expression.PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HCMVgH-8his (TB40/E)
Plasmid#136445PurposeMammalian expression plasmid for HCMV glycoprotein H (TB40/E strain) extracellular domain with 8his purification tagDepositorInsertglycoprotein H (UL75 Human betaherpesvirus 5)
UseTagsShort GGSG linker and 8his tagExpressionMammalianMutationExtracellular domain (a.a. 1-717). Codon-optimize…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HCMVgO-sfGFP (TB40/E)
Plasmid#136451PurposeMammalian expression plasmid for HCMV glycoprotein O (TB40/E strain) fused to superfolder GFPDepositorInsertglycoprotein O (UL74 Human betaherpesvirus 5)
UseTagsGly/Ser-rich linker (GGSGGSGSGG) (includes BamHI …ExpressionMammalianMutationCodon-optimized for human cell expression.PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HCMVgO-8his (TB40/E)
Plasmid#136453PurposeMammalian expression plasmid for HCMV glycoprotein O (TB40/E strain) with an 8his tagDepositorInsertenvelope glycoprotein O (UL74 Human betaherpesvirus 5)
UseTagsLinker and 8his tag (GGSGHHHHHHHH, includes BamHI…ExpressionMammalianMutationCodon-optimized for human cell expression.PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pDONR207 SARS-CoV-2 E
Plasmid#141273PurposeGateway-compatible Entry vector, with insert of the envelope (E) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1DepositorUseGateway-compatible entry vectorTagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailabilityIndustry, Academic Institutions, and Nonprofits -
pLenti-Zyxin-E (aa1-138)-EGFP
Plasmid#187697PurposeVisualization of ZyxinDepositorInsertZyxin (Zyx Mouse)
UseLentiviralTagsEGFPExpressionMammalianMutationaa1-138PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pDONR207 SARS-CoV-2 E 27nt-del
Plasmid#153954PurposeGateway-compatible Entry vector, with insert of E CDS bearing a 27nt deletion from SARS-CoV-2 genomic analysis in Davidson et al. 2020DepositorInsertE (E SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vectorTagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR207 SARS-CoV-2 E 27nt-del_nostop
Plasmid#153955PurposeGateway-compatible Entry vector, with insert of E CDS bearing a 27nt deletion from SARS-CoV-2 genomic analysis in Davidson et al. 2020DepositorInsertE (E SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vectorTagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailabilityIndustry, Academic Institutions, and Nonprofits
Showing: 21 - 40 of 4528 results