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Addgene
Showing: 21 - 40 of 4528 results
  1. pTS2334_Tier3(Lenti_inverse)-rtTA-YPet_Puro

    Plasmid
    #169665
    Purpose
    Tier-3 vector for lentiviral stable integration of the rtTA and puromyine - Ypet selection cassette in inverted direction with polyA (PRPBSA-YPet-p2a-PuroR::PPGK-rtTA-pA::A3-pA)
    Depositor
    Insert
    PPGK-driven rtTA expression and PRPBSA-driven YPet and PuroR expression
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PhCMV / PPGK / RPBSA
    Availability
    Academic Institutions and Nonprofits only
  2. pCAG-lox-inv[Talpha1-HA-Dre]-lox-mOrange2-NEDD1-gTBD

    Plasmid
    #196894
    Purpose
    Neuron-specific expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to mOrange aimed to displace endogenous gamma-TuRC from the centrosome. Used in combination with Talpha1-iCre-pA plasmids
    Depositor
    Insert
    N-gTBD-mOrange2 (NEDD1 Human)
    Use
    Cre/Lox; Dre/rox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Quimeric CAG
    Availability
    Academic Institutions and Nonprofits only
  3. pSPIKE-E

    Plasmid
    #101173
    Purpose
    chimeric spike resembling a fragment of eukaryotic 18S rRNA to be used in metagenomics
    Depositor
    Insert
    chimeric 18S rRNA
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  4. pcDNA3-neo-cterminal-3HA

    Plasmid
    #102643
    Purpose
    To generate three copies of the HA epitope tag on cDNAs.
    Depositor
    Type
    Empty backbone
    Use
    Tags
    3x HA (YPYDVPDYA)
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  5. pcDNA3-HCMVgH-8his (TB40/E)

    Plasmid
    #136445
    Purpose
    Mammalian expression plasmid for HCMV glycoprotein H (TB40/E strain) extracellular domain with 8his purification tag
    Depositor
    Insert
    glycoprotein H (UL75 Human betaherpesvirus 5)
    Use
    Tags
    Short GGSG linker and 8his tag
    Expression
    Mammalian
    Mutation
    Extracellular domain (a.a. 1-717). Codon-optimize…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  6. pDONR207 SARS-CoV-2 E

    Plasmid
    #141273
    Purpose
    Gateway-compatible Entry vector, with insert of the envelope (E) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1
    Depositor
    Has Service
    Cloning Grade DNA
    Insert
    E (E SARS-CoV-2 isolate Wuhan-Hu-1)
    Use
    Gateway-compatible entry vector
    Tags
    Expression
    Mutation
    Many synonymous changes due to codon optimization
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits
  7. pDONR207 SARS-CoV-2 E 27nt-del

    Plasmid
    #153954
    Purpose
    Gateway-compatible Entry vector, with insert of E CDS bearing a 27nt deletion from SARS-CoV-2 genomic analysis in Davidson et al. 2020
    Depositor
    Insert
    E (E SARS-CoV-2 isolate Wuhan-Hu-1)
    Use
    Gateway-compatible entry vector
    Tags
    Expression
    Mutation
    Many synonymous changes due to codon optimization
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits
  8. pDONR207 SARS-CoV-2 E 27nt-del_nostop

    Plasmid
    #153955
    Purpose
    Gateway-compatible Entry vector, with insert of E CDS bearing a 27nt deletion from SARS-CoV-2 genomic analysis in Davidson et al. 2020
    Depositor
    Insert
    E (E SARS-CoV-2 isolate Wuhan-Hu-1)
    Use
    Gateway-compatible entry vector
    Tags
    Expression
    Mutation
    Many synonymous changes due to codon optimization
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 21 - 40 of 4528 results