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pET21a-Chaetomium thermophilum LIC

Plasmid

#74782

Purpose

encodes dynein light intermediate chain (LIC) from Chaetomium thermophilum; no tag

Depositor

Ron Vale

Article

Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

Insert

Chaetomium thermophilum dynein light intermediate chain

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 16, 2016

Availability

Academic Institutions and Nonprofits only

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pET-15b(+) Sphaeforma arctica PRL (Δprenylation motif)

Plasmid

#166425

Purpose

Express pET-15b(+) Sphaeforma arctica PRL (Δprenylation motif)

Depositor

Kalle Gehring

Article

Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

Insert

Sphaeforma arctica PRL

Use

Tags

N-terminal His-tag

Expression

Bacterial

Mutation

Deleted nucleotides coding for 4 C-terminal amino…

Promoter

AmpR, lac1

Available since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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VV017: Archaerhodopsin-3 w/ D95Q point mutation fused to eGFP

Plasmid

#58490

Purpose

Expresses Arch(D95Q)-eGFP in mammalian cells as a fluorescent reporter of membrane voltage

Depositor

Adam Cohen

Article

Venkatachalam et al J Am Chem Soc. 2014 Feb 12;136(6):2529-3

Insert

Archaerhodopsin-3 w/ D95H point mutation fused to eGFP

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

Changed Aspartic Acid 95 to Glutamine

Promoter

Ubiquitin

Available since

Aug. 8, 2014

Availability

Academic Institutions and Nonprofits only

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pM9_fcpA_HD

Plasmid

#90416

Purpose

TALEN Backbone for Phaeodactylum tricornutum_ fcpA promotor_HD

Depositor

Peter Kroth

Article

Serif et al Algal Research Volume 23, April 2017, Pa

Insert

TALEN Backbone

Use

Phaeodactylum tricornutum

Tags

Expression

Mutation

Promoter

Available since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

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pM9_NR_HD

Plasmid

#90415

Purpose

TALEN Backbone for Phaeodactylum tricornutum_ NR promotor_HD

Depositor

Peter Kroth

Article

Serif et al Algal Research Volume 23, April 2017, Pa

Insert

TALEN Backbone

Use

Phaeodactylum tricornutum

Tags

Expression

Mutation

Promoter

Available since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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pM9_NR_NG

Plasmid

#90423

Purpose

TALEN Backbone for Phaeodactylum tricornutum_ NR promotor_NG

Depositor

Peter Kroth

Article

Serif et al Algal Research Volume 23, April 2017, Pa

Insert

TALEN Backbone

Use

Phaeodactylum tricornutum

Tags

Expression

Mutation

Promoter

Available since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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pM9_NR_NN

Plasmid

#90421

Purpose

TALEN Backbone for Phaeodactylum tricornutum_ NR promotor_NN

Depositor

Peter Kroth

Article

Serif et al Algal Research Volume 23, April 2017, Pa

Insert

TALEN Backbone

Use

Phaeodactylum tricornutum

Tags

Expression

Mutation

Promoter

Available since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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pM9_fcpA_NG

Plasmid

#90420

Purpose

TALEN Backbone for Phaeodactylum tricornutum_ fcpA promotor_NG

Depositor

Peter Kroth

Article

Serif et al Algal Research Volume 23, April 2017, Pa

Insert

TALEN Backbone

Use

Phaeodactylum tricornutum

Tags

Expression

Mutation

Promoter

Available since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

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pM9_fcpA_NN

Plasmid

#90419

Purpose

TALEN Backbone for Phaeodactylum tricornutum_ fcpA promotor_NN

Depositor

Peter Kroth

Article

Serif et al Algal Research Volume 23, April 2017, Pa

Insert

TALEN Backbone

Use

Phaeodactylum tricornutum

Tags

Expression

Mutation

Promoter

Available since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

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pM9_fcpA_NI

Plasmid

#90418

Purpose

TALEN Backbone for Phaeodactylum tricornutum_ fcpA promotor_NI

Depositor

Peter Kroth

Article

Serif et al Algal Research Volume 23, April 2017, Pa

Insert

TALEN Backbone

Use

Phaeodactylum tricornutum

Tags

Expression

Mutation

Promoter

Available since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

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pM9_NR_NI

Plasmid

#90422

Purpose

TALEN Backbone for Phaeodactylum tricornutum_ NR promotor_NI

Depositor

Peter Kroth

Article

Serif et al Algal Research Volume 23, April 2017, Pa

Insert

TALEN Backbone

Use

Phaeodactylum tricornutum

Tags

Expression

Mutation

Promoter

Available since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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pPtGE34

Plasmid

#107932

Purpose

Expresses Cas9 in Phaeodactylum tricornutum / Encodes elements required for conjugation

Depositor

David Edgell

Article

Slattery et al ACS Synth Biol. 2018 Jan 8. doi: 10.1021

Inserts

OriT
40SRPS8 Promoter
ShBle
40SRPS8 Terminator
Cen6-ArsH4-His3

Use

CRISPR and Synthetic Biology; Episomal vector for…

Tags

Expression

Mutation

Promoter

Available since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pPtGE35

Plasmid

#107999

Purpose

Expresses TevCas9 in Phaeodactylum tricornutum / Encodes elements required for conjugation

Depositor

David Edgell

Article

Slattery et al ACS Synth Biol. 2018 Jan 8. doi: 10.1021

Inserts

OriT
40SRPS8 Promoter
ShBle
40SRPS8 Terminator
Cen6-ArsH4-His3
I-TevI nuclease and partial linker domain

Use

CRISPR and Synthetic Biology; Episomal vector for…

Tags

Cas9

Expression

Mutation

Promoter

Available since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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HI.HslU.RSETB.fl.wt

Plasmid

#12556

Depositor

David McKay

Article

Trame et al Acta Crystallogr D Biol Crystallogr. 200

Insert

Haemophilus influenzae heat shock locus U

Use

Tags

Expression

Bacterial

Mutation

none

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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HI0058/pET-21a(+)c-tag

Plasmid

#13097

Depositor

SeWon Suh

Article

Ku et al Acta Crystallogr D Biol Crystallogr. 200

Insert

3-deoxy-manno-octulosonate cytidylyltransferase (kdsB Haemophilus influenzae)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

June 29, 2007

Availability

Academic Institutions and Nonprofits only

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pGS-21a-PcAad1

Plasmid

#163039

Purpose

expression of aryl-alcohol dehydrogenase from the white-rot fungus Phanerochaete chrysosporium

Depositor

Jean-Marie Francois

Article

Yang et al BMC Microbiol. 2012 Jun 28;12:126. doi:

Insert

aryl-alcohol dehydrogenase

Use

Tags

GST and His6

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 19, 2021

Availability

Academic Institutions and Nonprofits only

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SGL40C.EFS.dTomato

Plasmid

#89395

Purpose

Advanced lentiviral Vector for sgRNA (hU6 Promoter) delivery with dTomato expression (EFS Promoter)

Depositor

Dirk Heckl

Article

Reimer et al Haematologica. 2017 Jun 1. pii: haematol

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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L40C-CRISPR.EFS.dTomato

Plasmid

#89392

Purpose

Lentiviral CRISPR-Cas9 delivery for sgRNA (hU6), dTomato coexpression, EFS Promoter driven

Depositor

Dirk Heckl

Article

Reimer et al Haematologica. 2017 Jun 1. pii: haematol

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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SGL40C-H1.EFS.RFP657

Plasmid

#69148

Purpose

Advanced lentiviral Vector for sgRNA (H1 Promoter) delivery with RFP657 expression (EFS Promoter)

Depositor

Dirk Heckl

Article

Reimer et al Haematologica. 2017 Jun 1. pii: haematol

Inserts

sgRNA
EFS
tagRFP657

Use

Tags

Expression

Mutation

Promoter

Available since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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L40C-CRISPR.EFS.PAC

Plasmid

#89393

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA (hU6), PAC (Puromycin resistance) coexpression, EFS Promoter driven

Depositor

Dirk Heckl

Article

Reimer et al Haematologica. 2017 Jun 1. pii: haematol

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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