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pMSCVhyg-H3.3K4M-FLAG/HA

Plasmid

#128741

Purpose

Expression of histone H3.3K4M-FLAG/HA

Depositor

Kai Ge

Article

Jang et al Nucleic Acids Res. 2019 Jan 25;47(2):607

Insert

H3.3K4M (H3-3A Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mutation

K4M

Promoter

MSCV

Available since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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CMV-H3.3-YFP

Plasmid

#8693

Depositor

Kami Ahmad

Article

Janicki et al Cell 2004 Mar 5;116(5):683-98.

Insert

H3.3A (His3.3A Fly)

Use

Tags

YFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pQCXIpuro-H3.3K4M-FLAG/HA

Plasmid

#128742

Purpose

Expression of histone H3.3K4M-FLAG/HA

Depositor

Kai Ge

Article

Jang et al Nucleic Acids Res. 2019 Jan 25;47(2):607

Insert

H3.3K4M (H3-3A Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mutation

K4M

Promoter

CMV

Available since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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HS-H3.3A d436-GFP

Plasmid

#8697

Depositor

Kami Ahmad

Article

Ahmad et al Mol Cell 2002 Jun;9(6):1191-200.

Insert

H3.3A truncated (His3.3A Fly)

Use

Tags

GFP

Expression

Insect

Mutation

Deletion of amino acids 4-36.

Promoter

Available since

June 30, 2005

Availability

Academic Institutions and Nonprofits only

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CENPW H3.3 gRNA

Plasmid

#90620

Purpose

3rd generation lentiviral gRNA plasmid targeting human CENPW

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CENPW (Guide Designation H3.3)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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pLVX-H3.3K27M-FLAG-BFP

Plasmid

#209434

Purpose

Lentiviral packagable plasmid expressing FLAGGED human histone mutant H3.3-K27M with BFP

Depositor

Maria Castro

Insert

H3.3-K27M

Use

Tags

Expression

Mammalian

Mutation

K27M

Promoter

Available since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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pIRES-H3.3-WT-FLAG-BFP

Plasmid

#206139

Purpose

Expression of a FLAG tagged human histone H3.3 gene with BFP

Depositor

Maria Castro

Insert

H3.3 (H3-3A Human)

Use

Tags

FLAG-eBFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pKT-H3.3-K27M-katushka

Plasmid

#209443

Purpose

SB-transposable plasmid expressing a mutant histone H3.3-K27M in tandem with Katushka (RFP)

Depositor

Maria Castro

Insert

H3.3-K27M

Use

Tags

Expression

Mammalian

Mutation

K27M

Promoter

Available since

Jan. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-H3.3-polyA

Plasmid

#59780

Purpose

Express Flag-tagged H3.3 (H3F3B) in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

H3F3B (H3-3B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

Sept. 16, 2014

Availability

Academic Institutions and Nonprofits only

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pIHEU_miniSOG2 T2A H3.3A-EGFP

Plasmid

#87411

Purpose

UAS vector to express Histone 3.3A GFP fusion and miniSOG2 in Drosophila

Depositor

Xiaokun Shu

Article

Makhijani et al Cell Chem Biol. 2017 Jan 19;24(1):110-11

Insert

mini singlet oxygen generator 2

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pBT346.6-H3.3-K4M-bGHpolyA

Plasmid

#128743

Purpose

Inducible expression of histone H3.3K4M-FLAG/HA

Depositor

Kai Ge

Article

Jang et al Nucleic Acids Res. 2019 Jan 25;47(2):607

Insert

H3.3K4M (H3-3A Human)

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

K4M

Promoter

CAG

Available since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-H3.3(WT)-3AID-HA-2A-mTurquoiseBSD

Plasmid

#225701

Purpose

Lentiviral expression of AID degron tagged Wildtype H3.3 and mTurquoise fused BlasticidinR

Depositor

Lacra Bintu

Article

Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

Insert

H3.3 (WT) (H3-3A, H3-3B Human)

Use

Lentiviral

Tags

3xAID HA and T2A-mTurquoise

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-H3.3(WT)-3AID-HA-2A-mCherryBSD

Plasmid

#225697

Purpose

Lentiviral expression of AID degron tagged Wildtype H3.3 and mCherry fused BlasticidinR

Depositor

Lacra Bintu

Article

Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

Insert

H3.3 (WT) (H3-3A, H3-3B Human)

Use

Lentiviral and Synthetic Biology

Tags

3xAID HA and T2A-mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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px459-H3.3 (H3f3b) sgRNA

Plasmid

#186931

Purpose

H3f3b sgRNA used for genomic targeting at H3f3b C-terminal

Depositor

Zhiguo Zhang

Article

Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

Insert

H3f3b sgRNA (H3f3b Mouse)

Use

CRISPR and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-Histone H3.3 [N158/28R]

Plasmid

#182106

Purpose

Mammalian Expression Plasmid of anti-Histone H3.3 (Rat). Derived from hybridoma N158/28.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Histone H3.3 (Rattus norvegicus) recombinant mouse monoclonal antibody (H3f3b Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pIRES-H3.3-G34R-FLAG-BFP

Plasmid

#206140

Purpose

Expression of a FLAG tagged human histone mutant H3.3-G34R with BFP

Depositor

Maria Castro

Insert

H3.3 (H3-3A Human)

Use

Tags

FLAG-eBFP

Expression

Mammalian

Mutation

G34R

Promoter

Available since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-H3.3-G34R-FLAG-BFP

Plasmid

#209440

Purpose

Lentiviral packagable plasmid expressing FLAGGED human histone mutant H3.3-G34R with BFP

Depositor

Maria Castro

Insert

H3.3-G34R

Use

Tags

Expression

Mammalian

Mutation

G34R

Promoter

Available since

Nov. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-H3.3(K36M)-3AID-HA-2A-mTurquoiseBSD

Plasmid

#225703

Purpose

Lentiviral expression of AID degron tagged H3.3(K36M) and mTurquoise fused BlasticidinR

Depositor

Lacra Bintu

Article

Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

Insert

H3.3 (K36M) (H3-3A, H3-3B Human)

Use

Lentiviral and Synthetic Biology

Tags

3xAID HA and T2A-mTurquoise

Expression

Mammalian

Mutation

K36M

Promoter

pEF1A

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-H3.3(K27M)-3AID-HA-2A-mTurquoiseBSD

Plasmid

#225702

Purpose

Lentiviral expression of AID degron tagged H3.3(K27M) and mTurquoise fused BlasticidinR

Depositor

Lacra Bintu

Article

Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

Insert

H3.3 (K27M) (H3-3A, H3-3B Human)

Use

Lentiviral and Synthetic Biology

Tags

3xAID HA and T2A-mTurquoise

Expression

Mammalian

Mutation

K27M

Promoter

pEF1A

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-H3.3(K36M)-3AID-HA-2A-mCherryBSD

Plasmid

#225699

Purpose

Lentiviral expression of AID degron tagged H3.3(K36M) and mCherry fused BlasticidinR

Depositor

Lacra Bintu

Article

Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

Insert

H3.3 (K36M) (H3-3A, H3-3B Human)

Use

Lentiviral and Synthetic Biology

Tags

3xAID HA and T2A-mCherry

Expression

Mammalian

Mutation

K36M

Promoter

pEF1A

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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