Addgene

We narrowed to 156,664 results for: Gene

Showing: 21 - 40 of 156664 results

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pBS_KS_attB2_SA(1)_T2AGeneSwitch

Plasmid

#125215

Purpose

Artificial phase 1 exon to include a spliced T2A-GeneSwitch effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

Depositor

Herman Dierick

Article

Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

Insert

GeneSwitch

Use

Other

Tags

T2A

Expression

Mutation

Promoter

Available since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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M13KO7-ΔPS-ΔgeneIII

Plasmid

#134351

Purpose

Helper phage for M13 phage production

Depositor

Mark Isalan

Article

Brodel et al Sci Adv. 2020 Jun 10;6(24):eaba2728. doi

Insert

Helper phage gene

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 11, 2020

Availability

Academic Institutions and Nonprofits only

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pLITMUS*-PBBa_J23106-geneIII

Plasmid

#134352

Purpose

Phagemid

Depositor

Mark Isalan

Article

Brodel et al Sci Adv. 2020 Jun 10;6(24):eaba2728. doi

Insert

Gene III

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 11, 2020

Availability

Academic Institutions and Nonprofits only

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pRK5 BARK1 minigene

Plasmid

#14695

Depositor

Robert Lefkowitz

Article

Koch et al J Biol Chem. 1994 Feb 25. 269(8):6193-7.

Insert

BARK1 minigene (GRK2 Bovine)

Use

Tags

3'UTR of human b-globin

Expression

Mammalian

Mutation

BARK1 amino acids 495-689

Promoter

Available since

June 19, 2007

Availability

Academic Institutions and Nonprofits only

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GEARBOCS-SPARCL1-GeneTRAP

Plasmid

#218184

Purpose

To KO and genetrap mouse Hevin simultaneously

Depositor

Cagla Eroglu

Article

Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

Insert

sgRNA (Sparcl1 Mouse)

Use

AAV and CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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G608G lamin A minigene

Plasmid

#20292

Depositor

Tom Misteli

Article

Scaffidi et al Nat Med. 2005 Apr . 11(4):440-5.

Insert

lamin A (LMNA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

G606G:GGC>GGT Mutation causes aberrant spli…

Promoter

Available since

March 5, 2009

Availability

Academic Institutions and Nonprofits only
pEB2-mRuby3 Addgene

Plasmid

#104005

Purpose

Plasmid encoding mRuby3 Addgene

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mRuby3 Addgene

Use

Low copy

Tags

Expression

Bacterial

Mutation

Mutations relative to eqFP611 (MSKGEE LIKENMRMKVV…

Promoter

proC

Available since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pGene-TAP-GBP1

Plasmid

#138087

Purpose

Plasmid with TAP-GBP1 ORF

Depositor

Eva Frickel

Article

Fisch et al EMBO J. 2019 Jul 1;38(13):e100926. doi:

Insert

human GBP1 (GBP1 Human)

Use

Tags

Tandem affinity purification (TAP) tag

Expression

Mammalian

Mutation

Promoter

Gal4-E1b

Available since

April 7, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV5-FLAG-Gene33

Plasmid

#21633

Depositor

John Kyriakis

Article

Makkinje et al J Biol Chem. 2000 Jun 9. 275(23):17838-4

Insert

Gene33 (Errfi1 Rat)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only
Guide-Generating Vector

Plasmid

#125752

Purpose

Guide-Generating Vector for creating the Cys4-guide-scaffold structure in the first round of assembly

Depositor

Rodrigo Ledesma-Amaro

Article

McCarty et al ACS Synth Biol. 2019 Apr 2. doi: 10.1021

Insert

Cys4-GFPdropout-SpCas9scaffold

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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D5 gene in PETCON vector

Plasmid

#87626

Purpose

expresses D5 allosteric Zn2+ and fluorescein binding antibody for yeast display

Depositor

Sarel Fleishman

Article

Khersonsky et al Protein Sci. 2017 Apr;26(4):807-813. doi

Insert

Designed allosteric Zn2+ and fluorescein binding antibody scFv D5

Use

Tags

3x(G4S) Linker and cMyc tag on backbone

Expression

Yeast

Mutation

Promoter

Available since

March 29, 2017

Availability

Academic Institutions and Nonprofits only

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MPC11 IgG2b gene

Plasmid

#127248

Purpose

Expresses intact IgG2b gene in mammalian cells

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

IgG2b gene with exons and alt pAs (Ighg2b Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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CJ32 (let-7 minigene)

Plasmid

#17769

Depositor

David Bartel

Article

Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

Insert

dme-let-7 minigene (let-7 Fly)

Use

Drosophila p-element vector

Tags

Expression

Mutation

Promoter

Available since

May 12, 2008

Availability

Academic Institutions and Nonprofits only
CJ24 (let-7 minigene)

Plasmid

#17764

Depositor

David Bartel

Article

Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

Insert

dme-let-7 minigene (let-7 Fly)

Use

Drosophila metallothionein gene promoter drives e…

Tags

Expression

Insect

Mutation

Promoter

Available since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
GEARBOCS-Vamp2-GeneTRAP

Plasmid

#218187

Purpose

To KO and genetrap mouse Vamp2 simultaneously

Depositor

Cagla Eroglu

Article

Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

Insert

sgRNA (Vamp2 Mouse)

Use

AAV and CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGEARBOCS-v0-Gfap-GeneTRAP

Plasmid

#196492

Purpose

To KO and genetrap mouse GFAP simultaneously

Depositor

Cagla Eroglu

Article

Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

Inserts

mCherry-CAAX
gRNA

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGEARBOCS-v0-Vamp2-GeneTRAP

Plasmid

#196493

Purpose

To KO and genetrap mouse VAMP2 simultaneously

Depositor

Cagla Eroglu

Article

Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

Inserts

mCherry-CAAX
gRNA

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pUC19 gene 60 to gap

Plasmid

#49158

Purpose

Template for generating gene 60 mRNA, sequence 5' of coding gap only

Depositor

Nils Walter

Article

Todd et al RNA. 2013 May;19(5):685-700. doi: 10.126

Insert

Bacteriophage T4 gene 60

Use

Tags

Expression

Mutation

Coding gap and downstream sequence have been del…

Promoter

T7

Available since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pSC101-ΔPS-PM,CS-RBSBBa_B0034-geneVI

Plasmid

#134356

Purpose

Accessory plasmid

Depositor

Mark Isalan

Article

Brodel et al Sci Adv. 2020 Jun 10;6(24):eaba2728. doi

Insert

Gene VI

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 11, 2020

Availability

Academic Institutions and Nonprofits only

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tau exon 9–12 splicing minigene

Plasmid

#121165

Purpose

Generates circular RNAs from a Tau minigene

Depositor

Stefan Stamm

Article

Welden et al Biochim Biophys Acta Mol Basis Dis. 2018

Insert

Tau exons 9–12 (MAPT Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

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