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Plasmid#51793PurposeInducible expression of EBFP2 with rtTA for input fluorescenceDepositorInsertEBFP2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTREAvailable sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_m4_VPR_EBFP2
Plasmid#167881PurposePiggyBac compatible plasmid expressing dCas9-VPRDepositorInsertdCas9-VPR
UseCRISPRTagsExpressionMutationD10A, D839A, H840A, N863APromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_EBFP2
Plasmid#167857PurposePiggyBac compatible plasmid expressing spCas9DepositorInsertCas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQLinkHD_EBFP2
Plasmid#118853Purposefluorescent protein expression with an N-terminal His-tagDepositorInsertEBFP2
UseTagsHis-tagExpressionBacterialMutationEBFP1.2-I128V/V150I/ D155V/V224RPromoterAvailable sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_m4_KRAB_EBFP2
Plasmid#167873PurposePiggyBac compatible plasmid expressing dCas9-KRABDepositorInsertdCas9-KRAB
UseCRISPRTagsExpressionMutationD10A, D839A, H840A, N863APromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
12x_a1gRNAOp_pCYC1m_yeBFP2
Plasmid#64393Purposeencodes twelve a1 operator sites upstream of minimal pCYC1 promoter driving yeast enhanced BFPDepositorInsertyeast enhanced BFP2
UseTagsExpressionYeastMutationPromoterpCYC1Available sinceMay 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
6x_a1gRNAOp_pCYC1m_yeBFP2
Plasmid#64392Purposeencodes six a1 operator sites upstream of minimal pCYC1 promoter driving yeast enhanced BFPDepositorInsertyeast enhanced BFP2
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
3x_a1gRNAOp_pCYC1m_yeBFP2
Plasmid#64391Purposeencodes three a1 operator sites upstream of minimal pCYC1 promoter driving yeast enhanced BFPDepositorInsertyeast enhanced BFP2
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_VPR_EBFP2
Plasmid#167889PurposePiggyBac compatible plasmid expressing Cas9-VPRDepositorInsertCas9-VPR
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_m4_EBFP2
Plasmid#167865PurposePiggyBac compatible plasmid expressing dCas9DepositorInsertdCas9
UseCRISPRTagsExpressionMutationD10A, D839A, H840A, N863APromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEBFP2-Nuc
Plasmid#14893PurposeMammalian expression of EBFP2 blue fluorescent protein with NLSDepositorInsertpEBFP2-Nuc
UseTagsNLSExpressionMammalianMutationReplace EYFP of the vector pEYFP-Nuc from Clontec…PromoterAvailable sinceMay 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
EBFP2-H2B-6
Plasmid#55243PurposeLocalization: Nucleus/Histones, Excitation: 383, Emission: 448DepositorInsertH2B (H2BC11 Human)
UseTagsEBFP2ExpressionMammalianMutationD26G and V119I in H2BPromoterCMVAvailable sinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
EBFP2-Lysosomes-20
Plasmid#55246PurposeLocalization: Lysosome Membrane, Excitation: 383, Emission: 448DepositorInsertLAMP1
UseTagsEBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
EBFP2-Mito-7
Plasmid#55248PurposeLocalization: Mitochondria, Excitation: 383, Emission: 448DepositorInsertMito (COX8A Human)
UseTagsEBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 10, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.1-EBFP2(1-10)
Plasmid#165141PurposeExpresses EBFP2(1-10) in mammalian cellsDepositorInsertEBFP2(1-10)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 8, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
EBFP2-LaminB1-10
Plasmid#55244PurposeLocalization: Nuclear Envelope, Excitation: 383, Emission: 448DepositorInsertLaminB1 (LMNB1 Human)
UseTagsEBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
EBFP2-EB3-7
Plasmid#55239PurposeLocalization: MT End Binding Protein, Excitation: 383, Emission: 448DepositorInsertEB3 (MAPRE3 Human)
UseTagsEBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
EBFP2-Lifeact-7
Plasmid#54559PurposeLocalization: Actin, Excitation: 383, Emission: 448DepositorTypeEmpty backboneUseTagsEBFP2ExpressionMammalianMutationPromoterAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pQLinkHD_EBFP2-LSSmOrange
Plasmid#118856Purposefluorescent protein expression with an N-terminal His-tagDepositorInsertEBFP2-LSSmOrange
UseTagsHis-tagExpressionBacterialMutationEBFP1.2-I128V/V150I/ D155V/V224R and for LSSmOra…PromoterAvailable sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
EBFP2-Fibrillarin-7
Plasmid#55241PurposeLocalization: Nucleoli, Excitation: 383, Emission: 448DepositorInsertFibrillarin (FBL Human)
UseTagsEBFP2ExpressionMammalianMutationR36Q, S66FPromoterCMVAvailable sinceOct. 10, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits