Addgene

We narrowed to 35 results for: CyPet

Showing: 21 - 35 of 35 results

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C122-E16: CyPet

Plasmid

#162896

Purpose

Standard Gateway entry clone encoding CyPet fluorescent protein

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

CyPet fluorescent protein

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pCyPet-FHA2-AuroraB Substrate-YPet-IRES-puro2b

Plasmid

#40731

Depositor

Daniel Gerlich

Article

Wurzenberger et al J Cell Biol. 2012 Jul 23;198(2):173-83.

Insert

Aurora B FRET-sensor

Use

Tags

CyPet and YPet

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pH2B-CyPet-FHA2-AuroraB Substrate-YPet-IRES-puro2b

Plasmid

#40571

Depositor

Daniel Gerlich

Article

Wurzenberger et al J Cell Biol. 2012 Jul 23;198(2):173-83.

Insert

Aurora B FRET-sensor N-terminally fused to histone 2B

Use

Tags

CyPet and YPet

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pET15b-M1C

Plasmid

#107085

Purpose

M. sedula PCNA1 fused at the N-terminus of CyPet

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

M. sedula PCNA1 fused to CyPet

Use

Tags

His6 tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Availability

Academic Institutions and Nonprofits only

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pET15b-M2C

Plasmid

#107086

Purpose

M. sedula PCNA2 fused at the N-terminus of CyPet

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

M. sedula PCNA2 fused to CyPet

Use

Tags

His6 tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Availability

Academic Institutions and Nonprofits only

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pET15b-M3C

Plasmid

#107087

Purpose

M. sedula PCNA3 fused at the N-terminus of CyPet

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

M. sedula PCNA3 fused to CyPet

Use

Tags

His6 tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Availability

Academic Institutions and Nonprofits only

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pET15b-S1C

Plasmid

#107088

Purpose

S. solfataricus PCNA1 fused at the N-terminus of CyPet

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

S. solfataricus PCNA1 fused to CyPet

Use

Tags

His6 tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Availability

Academic Institutions and Nonprofits only

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pET15b-S3C

Plasmid

#107090

Purpose

S. solfataricus PCNA3 fused at the N-terminus of CyPet

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

S. solfataricus PCNA3 fused to CyPet

Use

Tags

His6 tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Availability

Academic Institutions and Nonprofits only

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pET15b-YC

Plasmid

#107072

Purpose

Host vector to construct a YPer or CyPet fusion protein

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

YPet-CyPet fusion protein

Use

Tags

His6 tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Availability

Academic Institutions and Nonprofits only

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pMMB67EH-CFP

Plasmid

#90103

Purpose

CFP control expression plasmid

Depositor

Samuel Miller

Article

Christen et al Science. 2010 Jun 4;328(5983):1295-7. do

Insert

CFP

Use

Broad-host range

Tags

Expression

Bacterial

Mutation

Promoter

Ptac

Availability

Academic Institutions and Nonprofits only

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Plk1 FRET sensor c-jun substrate

Plasmid

#45203

Depositor

Michael Lampson

Article

Liu et al J Cell Biol. 2012 Aug 20;198(4):491-9. d

Insert

Plk1 FRET sensor with c-jun substrate

Use

Tags

CyPET, YPET

Expression

Mammalian

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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N-term Hec1 Plk1 FRET sensor c-jun substrate

Plasmid

#45204

Depositor

Michael Lampson

Article

Liu et al J Cell Biol. 2012 Aug 20;198(4):491-9. d

Insert

Hec1-targeted Plk1 FRET sensor

Use

Tags

CyPET, YPET

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-E2

Plasmid

#175243

Purpose

Bacterial expression and purification, E2 enzyme (UBC9) for SUMOylation, can be recruited to FRB with rapamycin, CyPet acts as a FRET donor to Ypet

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

UBE2I (UBE2I Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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FKBP-YPet-RanGAP*

Plasmid

#175245

Purpose

Bacterial expression and purification, SUMOylation substrate that can be recruited to FRB with rapamycin, Ypet is a FRET acceptor for CyPet

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

RANGAP1 (RANGAP1 Human)

Use

Tags

Expression

Bacterial

Mutation

Amino acid 562 F to A

Promoter

tac

Availability

Academic Institutions and Nonprofits only

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untargeted Aurora B FRET sensor Kif2 substrate

Plasmid

#45215

Depositor

Michael Lampson

Article

Fuller et al Nature. 2008 Jun 19;453(7198):1132-6. do

Insert

untargeted Aurora B FRET sensor

Use

Tags

CyPET, YPET

Expression

Mammalian

Mutation

Promoter

t7

Availability

Academic Institutions and Nonprofits only

Enlarge