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pAAV-U6-DR(RfxCas13d)-CAG-hfCas13d-pa

Plasmid

#233036

Purpose

To express a RfxCas13d-compatible gRNA and to express HA Tagged hfCas13d from a CAG promoter

Depositor

Jonathan Ploski

Insert

RfxCas13d-compatible gRNA expression cassette and hfCas13d

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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spCas9-BlastR (pCBhCas9-BlastR)

Plasmid

#71489

Purpose

Expresses SpCas9 and Blasticidin resistance

Depositor

Richard Sherwood

Article

Arbab et al Stem Cell Reports. 2015 Oct 27. pii: S22

Insert

spCas9

Use

Tags

Expression

Mutation

Promoter

CBh

Available since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)

Plasmid

#140082

Purpose

Entry cloning vector for in vitro transcription or expression of SpCas9 sgRNAs from a T7 promoter

Depositor

Keith Joung

Insert

pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)

Use

In vitro transcription (mrna synthesis)

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-LwaCas13acrRNA-BsmBIcassette (LTH151)

Plasmid

#171129

Purpose

LwaCas13a crRNA entry vector for pU6 expression (need to clone in spacer into BsmBI sites)

Depositor

Benjamin Kleinstiver

Article

Johnson et al Mol Cell. 2022 Jun 2;82(11):2161-2166.e3

Insert

U6 promoter entry plasmid for LwaCas13a crRNA cloning

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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dSpyCas9-p300-P2A-CASANOVA

Plasmid

#113129

Purpose

Co-expresses dSpyCas9 fused to the p300 histone acetyltransferase core domain and CASANOVA

Depositor

Dominik Niopek

Article

Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

Insert

dCas9-p300-P2A-CASANOVA

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pRSF-DUET Cas1 Cas2

Plasmid

#80088

Purpose

Encodes Cas1 and Cas2

Depositor

George Church

Article

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1 (cas1 E. coli (K12))
Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pMS24: pCascade(PmcCAST)_entry

Plasmid

#168157

Purpose

Inducible expression of PmcCAST Cascade proteins. Two BsaI sites for spacer cloning.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

PmcCAST minimal CRISPR array
PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pMS7: pCascade(AvCAST)_entry

Plasmid

#168140

Purpose

Inducible expression of AvCAST Cascade proteins. Two BsaI sites for spacer cloning.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

AvCAST minimal CRISPR array
AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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Opto-casp9_casp9-VfAU1-LOV

Plasmid

#131222

Purpose

Expresses Opto-casp9_casp9-VfAU1-LOV (light-activated caspase9)

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

Opto-casp9 (casp9-VfAU1-LOV)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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Opto-casp9_casp9-AtCRY2-PHR

Plasmid

#131223

Purpose

Opto-casp9_casp9-AtCRY2-PHR (light-activated caspase9)

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

Opto-casp9 (casp9-AtCRY2-PHR)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pWUKI Cas1 P279L Cas2

Plasmid

#80095

Purpose

Encodes Cas1 P279L and Cas2

Depositor

George Church

Article

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1 (cas1 )
Cas2 (cas2 )
CRISPR Array

Use

Tags

Expression

Bacterial

Mutation

P279L

Promoter

Available since

Jan. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-DR(RfxCas13d)-CAG-CasRx-pa

Plasmid

#233035

Purpose

To express a RfxCas13d-compatible gRNA and to express HA Tagged CasRx from a CAG promoter

Depositor

Jonathan Ploski

Insert

RfxCas13d-compatible gRNA expression cassette and CasRx

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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SpCas9-NG-APOBEC-nCas9-Ung

Plasmid

#198539

Purpose

Expresses human codon-optimized SpCas9-NG-APOBEC-nCas9-Ung and blasticidin resistance: EFS promoter-SpCas9-NG-APOBEC-nCas9-Ung-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

Insert

SpCas9-NG-APOBEC-nCas9-Ung

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

Promoter

EFS

Available since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pWUKI Cas1 Cas2 E9Q

Plasmid

#80091

Purpose

Encodes Cas1 and Cas2 E9Q

Depositor

George Church

Article

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1 (cas1 )
Cas2 (cas2 )
CRISPR Array (bp1542-2899)

Use

Tags

Expression

Bacterial

Mutation

E9Q

Promoter

T7

Available since

Availability

Academic Institutions and Nonprofits only

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pWUKI Cas1 V134L Cas2

Plasmid

#80092

Purpose

Encodes Cas1 V134L and Cas2

Depositor

George Church

Article

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1 (cas1 )
Cas2 (cas2 )
CRISPR Array

Use

Tags

Expression

Bacterial

Mutation

V134L

Promoter

T7

Available since

Availability

Academic Institutions and Nonprofits only

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pWUKI Cas1 V33A Cas2

Plasmid

#80093

Purpose

Encodes Cas1 V33A and Cas2

Depositor

George Church

Article

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1 (cas1 )
Cas2 (cas2 )
CRISPR Array

Use

Tags

Expression

Bacterial

Mutation

V33A

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pWUKI Cas1 V190I Cas2

Plasmid

#80094

Purpose

Encodes Cas1 V190I and Cas2

Depositor

George Church

Article

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1 (cas1 )
Cas2 (cas2 )
CRISPR Array

Use

Tags

Expression

Bacterial

Mutation

V190I

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pWUKI Cas1 P202Q Cas2

Plasmid

#80096

Purpose

Encodes Cas1 P202Q and Cas2

Depositor

George Church

Article

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1 (cas1 )
Cas2 (cas2 )
CRISPR Array

Use

Tags

Expression

Bacterial

Mutation

P202Q

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pWUKI Cas1 P212L Cas2

Plasmid

#80097

Purpose

Encodes Cas1 P212L and Cas2

Depositor

George Church

Article

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1 (cas1 )
Cas2 (cas2 )
CRISPR Array

Use

Tags

Expression

Bacterial

Mutation

P212L

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pWUKI Cas1 P282L Cas2

Plasmid

#80098

Purpose

Encodes Cas1 P282L and Cas2

Depositor

George Church

Article

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1 (cas1 )
Cas2 (cas2 )
CRISPR Array

Use

Tags

Expression

Bacterial

Mutation

P282L

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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