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AAVS1_Puro_hPGK1_EGFP_Donor

Plasmid

#178088

Purpose

HDR donor plasmid to introduce the EGFP cassette to AAVS1 using AAVS1 T2 sgRNA.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

EGFP

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Human PGK1

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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PE7_AAVS1_knockin_HDR_donor

Plasmid

#223001

Purpose

HDR donor plasmid for PE7 knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PE7-IRES2-EGFP-WPRE-βglobinpA-right_homology_arm

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7

Use

Hdr donor

Tags

SV40 bpNLS and c-Myc NLS

Expression

Mammalian

Mutation

Promoter

EF1α

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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PEmax_AAVS1_knockin_HDR_donor

Plasmid

#223000

Purpose

HDR donor plasmid for PEmax knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PEmax-IRES2-EGFP-WPRE-βglobinpA-right_homology_arm

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PEmax

Use

Hdr donor

Tags

SV40 bpNLS and c-Myc NLS

Expression

Mammalian

Mutation

Promoter

EF1α

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1_Puro_PGK1_3xFLAG_Twin_Strep

Plasmid

#68375

Purpose

Doyon lab Tandem-Affinity Purification Following (i) Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus and (ii) Endogenous TAP Tagging.

Depositor

Yannick Doyon

Article

Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR and TALEN; Zfn

Tags

3xFLAG_Twin_Strep

Expression

Mammalian

Mutation

Promoter

human PGK1

Available since

Oct. 13, 2015

Availability

Academic Institutions and Nonprofits only

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gRNA_AAVS1-T1

Plasmid

#41817

Purpose

Expresses a guide RNA (gRNA) to target human AAVS1 (T1 target sequence) for genome engineering

Depositor

George Church

Article

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Insert

gRNA_AAVS1-T1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR_Puro_sgAAVS1

Plasmid

#187818

Purpose

pLentiCRISPR that is selectable with puromycin with an sgRNA targeting AAVS1

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

sgAAVS1

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pLCKO_AAVS1_sgRNA_2

Plasmid

#155085

Purpose

lentiviral plasmid expressing Cas9 gRNA targeting AAVS1 safe harbor locus

Depositor

Jason Moffat

Article

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Insert

AAVS1_sgRNA_2

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6 promoter

Available since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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plentiCRISPv2_AAVS1

Plasmid

#184487

Purpose

Lentivirus all in one CRISPR vector targeting human AAVS1 gene

Depositor

Todd Golub, Peter Tsvetkov

Article

Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

Insert

AAVS1 (AAVS1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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AAVS1_Puro_hPGK1_EBFP_Donor

Plasmid

#178089

Purpose

HDR donor plasmid to introduce the EBFP (T65S/F145Y) cassette to AAVS1 using AAVS1 T2 sgRNA.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

EBFP (T65S/F145Y)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Human PGK1

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pDECKO_GFP_AAVS1_186

Plasmid

#159091

Purpose

targeting, non-essential locus control

Depositor

Rory Johnson

Insert

gRNA

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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AAVS1_gRNA

Plasmid

#196139

Purpose

gRNA targeting the AAVS1 locus in a third generation Cas9 backbone with GFP

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

AAVS1 gRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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AAVS1_sgRNA

Plasmid

#100554

Purpose

Expresses AAVS1 sgRNA. Target sequence: GGGGCCACTAGGGACAGGAT

Depositor

Dustin Maly

Article

Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

Insert

AAVS1 sgRNA

Use

Tags

Expression

Mutation

Promoter

U6

Available since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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AAVS1_puro_CAG_HexaPro

Plasmid

#164077

Purpose

Targeted integration of SARS-CoV-2 spike HexaPro variant into the AAVS1 genomic safe harbor locus

Depositor

Yannick Doyon

Article

Rivest et al protocols.io 2021

Insert

SARS-CoV-2 S HexaPro (S Severe acute respiratory syndrome coronavirus 2)

Use

CRISPR

Tags

2X Strep-Tag II, 8X His tag, and HRV 3C cleavage …

Expression

Mammalian

Mutation

Ectodomain only (AAs 1-1208); 682-685 (furin site…

Promoter

CAG

Available since

Jan. 4, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pAAVS1_Puro_ccdb_Ubc_rtTA

Plasmid

#172130

Purpose

Destination vector for AAVS1 targeting

Depositor

Kenji Kontani

Article

Araki et al J Biol Chem. 2021 Nov 18:101428. doi: 10

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

PGK1

Available since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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AAVS1_Puro_PGK1_3xFLAG_Twin_Strep_EZH2

Plasmid

#79902

Purpose

Gene Addition of 3xFLAG_Twin_Strep_EZH2 to the AAVS1 Genomic Safe Harbor Locus

Depositor

Yannick Doyon

Article

Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

Insert

3xFLAG_Twin_Strep_EZH2

Use

CRISPR and TALEN

Tags

3xFLAG_Twin_Strep

Expression

Mammalian

Mutation

Promoter

PGK1

Available since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pDECKO_mCherry_AAVS1

Plasmid

#141212

Purpose

pDECKO_mCherry_AAVS1_186. Targeting, non-essential locus control

Depositor

Rory Johnson

Insert

gRNA

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

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psAAVS1-T2

Plasmid

#60543

Purpose

GFP reporter for CRISPR/CAS9 and gRNA_AAVS1-T2 activities

Depositor

William Simonds

Article

Zhang et al J Biotechnol. 2014 Sep 2. pii: S0168-165

Insert

ProtoSpacer AAVS1-T2 (AAVS1 Human)

Use

CRISPR; Kan

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pDECKO_GFP_AAVS1

Plasmid

#141211

Purpose

pDECKO_GFP_AAVS1_1801. Targeting, non-essential locus control

Depositor

Rory Johnson

Article

Esposito et al Cell Genom. 2022 Aug 22;2(9):100171. doi

Insert

gRNA

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

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LCV2_mClover3_AAVS1_sgRNA_2

Plasmid

#155099

Purpose

lentiviral plasmid expressing mClover3, Cas9 and a gRNA targeting the AAVS1 safe harbor locus

Depositor

Jason Moffat

Article

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Insert

AAVS1_sgRNA_2

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6 promoter

Available since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_AAVS1A

Plasmid

#183337

Purpose

All-in-One CRISPRko system with a guide RNA that targets the AAVS1 locus

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

AAVS1A

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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