1. pU6-(BbsI)_CBh-Cas9-T2A-mCherry

   Plasmid

   #64324

   Purpose

   Expression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to mCherry via a T2A peptide

   Depositor

   Ralf Kuehn

   Article

   Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

   Inserts

   Cas9
   sgRNA cassette
   

   Use

   CRISPR

   Tags

   3xFLAG, NLS, and T2A-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available since

   Sept. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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1. pSICO-CPSF6-358-iRFP670

   Plasmid

   #110694

   Purpose

   Expresses a truncated form of CPSF6 (CPSF6-358 originally described in Lee et al. Cell Host Microbe 2010) fused with iRFP670

   Depositor

   Zandrea Ambrose

   Article

   Ning et al J Virol. 2018 Apr 11. pii: JVI.00368-18.

   Insert

   CPSF6-iRFP670 (CPSF6 Human)
   

   Use

   Lentiviral

   Tags

   iRFP670

   Expression

   Mutation

   Truncation of CPSF6 isoform at amino acid 358

   Promoter

   Available since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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