1. hCas9

   Plasmid

   #41815

   Purpose

   Expresses human codon optimized Cas9 nuclease for genome engineering

   Depositor

   George Church

   Article

   Mali et al Science. 2013 Feb 15;339(6121):823-6. do

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   human codon-optimized

   Promoter

   CMV

   Available since

   Jan. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

These results may also be relevant.

1. pTKhc-yibDp-GFP-β20cp6

   Plasmid

   #127060

   Purpose

   Low phosphate inducible circular permutation of GFP, which is a substrate of the lon protease

   Depositor

   Michael Lynch

   Article

   Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

   Insert

   GFPcp6-beta20
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   yibD

   Available since

   May 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHCKan-yibDp-CBD-hGLY

   Plasmid

   #134596

   Purpose

   Low Phosphate inducible hGLYAT2 with N-terminal chitin binding domain tag

   Depositor

   Michael Lynch

   Article

   Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

   Insert

   human glycine acyltransferase 2 (GLYATL2 )
   

   Use

   Tags

   chitin binding domain

   Expression

   Bacterial

   Mutation

   Promoter

   yibD

   Available since

   May 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSMART-Ala1

   Plasmid

   #65814

   Purpose

   NADPH dependent Alanine Dehydrogenase, Low Phosphate Dependent E. coli Expression

   Depositor

   Michael Lynch

   Article

   Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

   Insert

   NADPH Alanine Dehydrogenase (ald Synthetic)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D196A, L197R

   Promoter

   Insulated waaHp from E. coli

   Available since

   July 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV4Neo-murine IL-17RA-HA

   Plasmid

   #46859

   Purpose

   expresses mouse IL-17RA

   Depositor

   Sarah Gaffen

   Article

   Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

   Insert

   IL-17RA (Il17ra Mouse)
   

   Use

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pSMART-ala10

   Plasmid

   #87135

   Purpose

   NADPH dependent Alanine Dehydrogenase, Low Phosphate Dependent E. coli Expression

   Depositor

   Michael Lynch

   Article

   Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

   Inserts

   NADPH Alanine Dehydrogenase (ald Synthetic)
   alanine exporter (alaE Synthetic)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D196A, L197R

   Promoter

   waaHp from E. coli as part of operon with ald* an…

   Available since

   March 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. gh33

   Plasmid

   #106710

   Purpose

   expression of gRNA targeting B4GALNT3

   Depositor

   Eric-Paul Bennett

   Article

   Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

   Insert

   B4GALNT3 (B4GALNT3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV4Neo-murine IL-17RASEFIR-HA

   Plasmid

   #46863

   Purpose

   expresses mouse IL-17RA with SEFIR deletion

   Depositor

   Sarah Gaffen

   Article

   Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

   Insert

   IL-17RA-delta-SEFIR (Il17ra Mouse)
   

   Use

   Tags

   3x HA

   Expression

   Mammalian

   Mutation

   Deletion of SEFIR domain (AA 394-485)

   Promoter

   CMV

   Available since

   Sept. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

8. CL20_mEGFP_ADIPOR2_FOXJ2

   Plasmid

   #205768

   Purpose

   Express mEGFP-tagged fusion protein, ADIPOR2_FOXJ2 from patient-derived sequence

   Depositor

   Richard Kriwacki

   Article

   Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

   Insert

   ADIPOR2_FOXJ2 (ADIPOR2, FOXJ2 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLV-Enh eGFP Reporter-muKC2-X6

   Plasmid

   #59230

   Purpose

   Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.

   Depositor

   Benjamin Yu

   Insert

   krt5 ex2
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Mu Hsp68 minimal promoter

   Available since

   Oct. 1, 2014

   Availability

   Academic Institutions and Nonprofits only