1. HDAC3 Flag

   Plasmid

   #13819

   Purpose

   Mammalian expression of human histone deacetylase 3 with flag tag

   Depositor

   Eric Verdin

   Article

   Emiliani et al Proc Natl Acad Sci U S A. 1998 Mar 17. 9

   Insert

   HDAC3 (HDAC3 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

These results may also be relevant.

1. HA-HIF2α MT-pBabe-Hygro

   Plasmid

   #22134

   Depositor

   William Kaelin

   Article

   Kondo et al PLoS Biol. 2003 Dec . 1(3):E83.

   Insert

   Hypoxia inducible factor 2 alpha (EPAS1 Human)
   

   Use

   Retroviral

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   cDNA "silent"("conservative"…

   Promoter

   Available since

   Oct. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. HA-HIF2α wt-pBabe-Puro

   Plasmid

   #26055

   Depositor

   William Kaelin

   Article

   Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

   Insert

   Hypoxia inducible factor 2 alpha (EPAS1 Human)
   

   Use

   Retroviral

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   wt cDNA

   Promoter

   Available since

   Aug. 20, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. HA-HIF2α P405A;P531A;bHLH*-pBabe-Puro

   Plasmid

   #22135

   Depositor

   William Kaelin

   Article

   Kondo et al PLoS Biol. 2003 Dec . 1(3):E83.

   Insert

   Hypoxia inducible factor 2 alpha (EPAS1 Human)
   

   Use

   Retroviral

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   cDNA changed amino acids from wt: Proline 405 t…

   Promoter

   Available since

   Nov. 10, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pg-dsRedXP-HIF-2alpha

   Plasmid

   #87209

   Purpose

   N-terminally dsRedXP-tagged human HIF-2a/EPAS

   Depositor

   Violaine Sée

   Article

   Taylor et al Open Biol. 2016 Sep;6(9). pii: 160195.

   Insert

   HIF-2a (EPAS1 Human)
   

   Use

   Tags

   dsRedXP

   Expression

   Mammalian

   Mutation

   Q868E

   Promoter

   CMV

   Available since

   Sept. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. HA-HIF2alpha-P405A/P531A-pcDNA3

   Plasmid

   #18956

   Depositor

   William Kaelin

   Article

   Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

   Insert

   Hypoxia inducible factor 2 alpha (EPAS1 Human)
   

   Use

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   cDNA changed amino acids: Proline 405 to Alanin…

   Promoter

   Available since

   Aug. 20, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. HA-HIF2alpha-P405A/P531A-pBabe-puro

   Plasmid

   #19006

   Depositor

   William Kaelin

   Article

   Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

   Insert

   Hypoxia inducible factor 2 alpha (EPAS1 Human)
   

   Use

   Retroviral

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   cDNA changed amino acids: P405A and P531A

   Promoter

   Available since

   Aug. 20, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3 mHIF-2α MYC (P405A/P530V/N851A)

   Plasmid

   #44027

   Depositor

   Celeste Simon

   Article

   Hu et al Mol Biol Cell. 2007 Nov;18(11):4528-42.

   Insert

   mHIF-2α (P405A/P530V/N851A) (Epas1 Mouse)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   P405A, P530V, N851A

   Promoter

   CMV

   Available since

   March 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pg-EGFP-HIF-2alpha

   Plasmid

   #87207

   Purpose

   N-terminally EGFP-tagged human HIF-2a/EPAS

   Depositor

   Violaine Sée

   Article

   Bagnall et al J Biol Chem. 2014 Feb 28;289(9):5549-64.

   Insert

   HIF-2a (EPAS1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Q868E

   Promoter

   CMV

   Available since

   Sept. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. HA-HIF2α P405A/P531A-pBabe-Hygro

   Plasmid

   #21676

   Depositor

   William Kaelin

   Article

   Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

   Insert

   Hypoxia inducible factor 2 alpha (EPAS1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   cDNA changed amino acids from wt: Proline 405 t…

   Promoter

   Available since

   Sept. 4, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. HA-HIF2α P405A/P531A/N847A-pBabe-Puro

    Plasmid

    #25956

    Depositor

    William Kaelin

    Article

    Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

    Insert

    Hypoxia inducible factor 2 alpha (EPAS1 Human)
    

    Use

    Retroviral

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    cDNA changed amino acids: Proline 405 to Alanin…

    Promoter

    Available since

    Oct. 19, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. HA-HIF2α P405A Δ450-572-pBabe-Puro

    Plasmid

    #25958

    Depositor

    William Kaelin

    Article

    Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

    Insert

    Hypoxia inducible factor 2 alpha (EPAS1 Human)
    

    Use

    Retroviral

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    cDNA changed amino acids: Proline 405 to Alanin…

    Promoter

    Available since

    Sept. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. HA-HIF2α P405A/P531A/N847Q-pBabe-Puro

    Plasmid

    #25948

    Depositor

    William Kaelin

    Article

    Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

    Insert

    Hypoxia inducible factor 2 alpha (EPAS1 Human)
    

    Use

    Retroviral

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    cDNA changed amino acids: Proline 405 to Alanin…

    Promoter

    Available since

    Sept. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pIS1 EPAS1

    Plasmid

    #60792

    Purpose

    Luciferase reporter to measure miR-155 mediated differential repression. Contains EPAS1 3' UTR and wild-type miR-155 sites

    Depositor

    David Bartel

    Article

    Nam et al Mol Cell. 2014 Mar 20;53(6):1031-43. doi

    Insert

    EPAS1 3'UTR and wild-type miR-155 binding site (EPAS1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    thymidine kinase (HSV-TK promoter)

    Available since

    Oct. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pIS1 mEPAS1

    Plasmid

    #60793

    Purpose

    Luciferase reporter to measure miR-155 mediated differential repression. Contains EPAS1 3' UTR and mutated miR-155 sites

    Depositor

    David Bartel

    Article

    Nam et al Mol Cell. 2014 Mar 20;53(6):1031-43. doi

    Insert

    EPAS1 3'UTR and mutated miR-155 binding site (EPAS1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    mutated miR-155 binding site

    Promoter

    thymidine kinase (HSV-TK promoter)

    Available since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. TFORF1106

    Plasmid

    #143762

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    EPAS1 (EPAS1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCDH-CB1-HIF2a-GFP-T2A-Puro

    Plasmid

    #71708

    Purpose

    Lentiviral expression of HIF2A-GFP

    Depositor

    Xiande Liu, Eric Jonasch

    Article

    Liu et al Oncogene. 2015 May 7;34(19):2450-60. doi

    Insert

    HIF2A (EPAS1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. HA-HIF2α P405A/P531A Δ820-870-pBabe-Puro

    Plasmid

    #25949

    Depositor

    William Kaelin

    Article

    Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

    Insert

    Hypoxia inducible factor 2 alpha (EPAS1 Human)
    

    Use

    Retroviral

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    cDNA changed amino acids: Proline 405 to Alanin…

    Promoter

    Available since

    Oct. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. HA-HIF2α P405A Δ450-572/Δ820-870-pBabe-Puro

    Plasmid

    #25955

    Depositor

    William Kaelin

    Article

    Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

    Insert

    Hypoxia inducible factor 2 alpha (EPAS1 Human)
    

    Use

    Retroviral

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    cDNA changed amino acids: Proline 405 to Alanin…

    Promoter

    Available since

    Sept. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HA-HIF2α bHLH* P405A/P531A Δ820-870-pBabe-Puro

    Plasmid

    #25957

    Depositor

    William Kaelin

    Article

    Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

    Insert

    Hypoxia inducible factor 2 alpha (EPAS1 Human)
    

    Use

    Retroviral

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    cDNA changed amino acids: Proline 405 to Alanin…

    Promoter

    Available since

    Sept. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. HA-HIF2α P405A/N847A Δ450-572-pBabe-Puro

    Plasmid

    #25936

    Depositor

    William Kaelin

    Article

    Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

    Insert

    Hypoxia inducible factor 2 alpha (EPAS1 Human)
    

    Use

    Retroviral

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    cDNA changed amino acids: Proline 405 to Alanin…

    Promoter

    Available since

    Sept. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge