1. pCL-Eco

   Plasmid

   #12371

   Depositor

   Inder Verma

   Article

   Naviaux et al J Virol. 1996 Aug . 70(8):5701-5.

   Insert

   gag/pol/env
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

These results may also be relevant.

1. pCRISPR Human CASP9 -1

   Plasmid

   #198419

   Purpose

   Expresses Human CASP9 Specific gRNA/Cas9 Complex and Reporter Protein

   Depositor

   Hannah Rabinowich

   Article

   Han et al Autophagy. 2021 Jun;17(6):1393-1409. doi

   Insert

   Human CASP9 Specific gRNA (CASP9 Human)
   

   Use

   CRISPR

   Tags

   Cas9/Orange Fluorescent Protein Reporter

   Expression

   Mammalian

   Mutation

   Promoter

   U6; CMV

   Available since

   April 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMMP-MCS-IRES-eGFP-P2A-iCas9

   Plasmid

   #178533

   Purpose

   For genome editing: EGFP-inducible Caspase9 knock-in cassette which allows ablation of target cells

   Depositor

   Ryoji Yao

   Article

   Okamoto et al STAR Protoc. 2021 Sep 16;2(4):100780. do

   Insert

   iCaspase9 (CASP9 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   FKBP12 F36 to V Truncated Caspase9 (135-416; lack…

   Promoter

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Tet-Inducible Human Caspase-9 Alpha

   Plasmid

   #194708

   Purpose

   Inducible Expression of Human Caspase-9 Alpha

   Depositor

   Hannah Rabinowich

   Article

   Han et al J Biol Chem. 2014 Mar 7;289(10):6485-649

   Insert

   Human Caspase-9 Alpha (CASP9 Human)
   

   Use

   Tags

   None

   Expression

   Mammalian

   Mutation

   None

   Promoter

   CMV

   Available since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Mouse DsRed-Monomer CASP9 C325A Mutant

   Plasmid

   #198401

   Purpose

   Expresses Mouse DsRed-Monomer CASP9 C325A Mutant Fusion Protein in Mammalian Cells

   Depositor

   Hannah Rabinowich

   Article

   Han et al Autophagy. 2021 Jun;17(6):1393-1409. doi

   Insert

   Mouse Caspase-9 C325A Mutant (Casp9 Mouse)
   

   Use

   Tags

   DsRed-monomer

   Expression

   Mammalian

   Mutation

   Changed Cysteine 325 to Alanine to eliminate prot…

   Promoter

   CMV

   Available since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. N-terminal 3X Flag Human CASP9 C287A Mutant

   Plasmid

   #198380

   Purpose

   Expresses Human N-Terminal 3X Flag CASP9 Catalytic Mutant in Mammalian Cells

   Depositor

   Hannah Rabinowich

   Article

   Han et al Autophagy. 2021 Jun;17(6):1393-1409. doi

   Insert

   N-Terminal 3X Flag Human Caspase-9 C287A Mutant (CASP9 Human)
   

   Use

   Tags

   3X Flag

   Expression

   Mammalian

   Mutation

   Changed Cysteine 287 to Alanine to eliminate prot…

   Promoter

   CMV

   Available since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Tet-Inducible Mouse C325A Caspase-9 Mutant

   Plasmid

   #194500

   Purpose

   Inducible Expression of Mouse C325A Caspase-9 Mutant

   Depositor

   Hannah Rabinowich

   Article

   Han et al J Biol Chem. 2014 Mar 7;289(10):6485-649

   Insert

   Mouse Caspase-9 C325A Mutant (Casp9 Mouse)
   

   Use

   Tags

   None

   Expression

   Mammalian

   Mutation

   Changed Cysteine 325 to Alanine to eliminate prot…

   Promoter

   CMV

   Available since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Tet-Inducible Human Caspase-9 Alpha C287A Mutant

   Plasmid

   #194709

   Purpose

   Inducible Expression of Human Caspase-9 Alpha C287A Mutant

   Depositor

   Hannah Rabinowich

   Article

   Han et al J Biol Chem. 2014 Mar 7;289(10):6485-649

   Insert

   Human Caspase-9 C287A Mutant (CASP9 Human)
   

   Use

   Tags

   None

   Expression

   Mammalian

   Mutation

   Changed Cysteine 287 to Alanine to eliminate prot…

   Promoter

   CMV

   Available since

   Feb. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Tet-inducible 3xFlag Mouse C325A Caspase-9 Mutant

   Plasmid

   #194501

   Purpose

   Inducible Expression of N-Terminal 3X FLAG Mouse Caspase-9 C325A Mutant

   Depositor

   Hannah Rabinowich

   Article

   Han et al J Biol Chem. 2014 Mar 7;289(10):6485-649

   Insert

   Caspase-9 C325A Mutant (Casp9 Mouse)
   

   Use

   Tags

   3X Flag

   Expression

   Mammalian

   Mutation

   Changed Cysteine 325 to Alanine to eliminate prot…

   Promoter

   CMV

   Available since

   Feb. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Tet-inducible 3xFlag Mouse Caspase-9 ΔLS

   Plasmid

   #194609

   Purpose

   Inducible Expression of N-Terminal 3X Flag Mouse Caspase-9 Minus Large Subunit Domain

   Depositor

   Hannah Rabinowich

   Article

   Han et al J Biol Chem. 2014 Mar 7;289(10):6485-649

   Insert

   N-Terminal 3X Flag Mouse Caspase-9 Minus Sequence Encoding Val 177 to Asp 353 (Casp9 Mouse)
   

   Use

   Tags

   3X Flag

   Expression

   Mammalian

   Mutation

   Deletion of Large Subunit Domain eliminates bindi…

   Promoter

   CMV

   Available since

   Feb. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Tet-inducible 3xFlag Mouse C325A Caspase-9 ΔCARD

    Plasmid

    #194608

    Purpose

    Inducible Expression of N-Terminal 3X Flag Mouse C325A Caspase-9 Mutant Minus CARD Domain

    Depositor

    Hannah Rabinowich

    Article

    Han et al J Biol Chem. 2014 Mar 7;289(10):6485-649

    Insert

    N-Terminal 3X Flag Mouse C325A Caspase-9 Mutant Minus Sequence Encoding Met 1 to Lys 176 (Casp9 Mouse)
    

    Use

    Tags

    3X Flag

    Expression

    Mammalian

    Mutation

    Deletion of N-terminal CARD domain and changed Cy…

    Promoter

    CMV

    Available since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Tet-inducible 3xFlag Mouse C325A Caspase-9 ΔA LS

    Plasmid

    #194926

    Purpose

    Inducible Expression of N-Terminal 3X Flag Mouse C325A Caspase-9 Minus Large Subunit Region Val 177 to Arg 263

    Depositor

    Hannah Rabinowich

    Article

    Han et al J Biol Chem. 2014 Mar 7;289(10):6485-649

    Insert

    N-Terminal 3X Flag Mouse C325A Caspase-9 Minus Sequence Encoding Val 177 to Arg 263 (Casp9 Mouse)
    

    Use

    Tags

    3X Flag

    Expression

    Mammalian

    Mutation

    Changed Cysteine 325 to Alanine to Eliminate Prot…

    Promoter

    CMV

    Available since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Tet-inducible 3xFlag Mouse C325A Caspase-9 ΔLD + ΔSS

    Plasmid

    #194610

    Purpose

    Inducible Expression of N-Terminal 3X Flag Mouse C325A Caspase-9 Minus Linker + Small Subunit Domain

    Depositor

    Hannah Rabinowich

    Article

    Han et al J Biol Chem. 2014 Mar 7;289(10):6485-649

    Insert

    N-terminal 3X Flag Mouse C325A Caspase-9 Minus Sequence Encoding Ala 354 to Ser 454 (Casp9 Mouse)
    

    Use

    Tags

    3X Flag

    Expression

    Mammalian

    Mutation

    Changed Cysteine 325 to Alanine to eliminate prot…

    Promoter

    CMV

    Available since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Tet-inducible 3xFlag Mouse Caspase-9 ΔB LS

    Plasmid

    #194927

    Purpose

    Inducible Expression of N-Terminal 3X Flag Mouse Caspase-9 Minus Large Subunit Region Ala 264 to Asp 353

    Depositor

    Hannah Rabinowich

    Article

    Han et al J Biol Chem. 2014 Mar 7;289(10):6485-649

    Insert

    N-Terminal 3X Flag Mouse Caspase-9 Minus Sequence Encoding Ala 264 to Asp 353 (Casp9 Mouse)
    

    Use

    Tags

    3X Flag

    Expression

    Mammalian

    Mutation

    Deletion of Large Subunit Region Ala 264 to Asp 3…

    Promoter

    CMV

    Available since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pELF4-donor

    Plasmid

    #112371

    Purpose

    CRISPR donor plasmid to tag human transcription factor ELF4 with GFP

    Depositor

    Kevin White

    Insert

    ELF4 homology arms flanking EGFP-IRES-Neo cassette (ELF4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTwist-SARS-CoV-2 Spike-deltaC18 M1229I (ATC)

    Plasmid

    #212371

    Purpose

    Encodes SARS-CoV-2 spike M1229I for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike M1229I (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    M1229I

    Promoter

    CAG

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. HA-ISceID44A

    Plasmid

    #59424

    Purpose

    Expresses a catalytically inactive form of the endonuclease ISceI

    Depositor

    Tom Misteli

    Article

    Roukos et al Science. 2013 Aug 9;341(6146):660-4. doi

    Insert

    endonuclease ISceI mutant (I-SceI )
    

    Use

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    changed aspartate 44 to alanine (D44A)

    Promoter

    Available since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pQCXIP-GFP-LacR

    Plasmid

    #59418

    Purpose

    retroviral construct expressing the Lac repressor (LacR) tagged with GFP

    Depositor

    Tom Misteli

    Article

    Roukos et al Science. 2013 Aug 9;341(6146):660-4. doi

    Insert

    Lac repressor (LacR)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    Nov. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pQCXIN-TetR-mCherry

    Plasmid

    #59417

    Purpose

    retroviral construct expressing the Tet repressor (TetR) tagged with mCherry

    Depositor

    Tom Misteli

    Article

    Roukos et al Science. 2013 Aug 9;341(6146):660-4. doi

    Insert

    Tet repressor protein (TetR)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available since

    Nov. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMXLK_dFe

    Plasmid

    #114233

    Purpose

    POC12371, MetClo assembly vector with p15A replication origin and kanamycin resistance for BsaI-based MetClo, adaptor sequence type d-e

    Depositor

    Christopher A. O'Callaghan

    Article

    Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

    Insert

    LacZalpha
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLacO-ISce1

    Plasmid

    #58505

    Purpose

    provides 256 Lac operator repeats adjacent to the ISce1 restriction site

    Depositor

    Tom Misteli

    Article

    Roukos et al Science. 2013 Aug 9;341(6146):660-4. doi

    Insert

    Lac repeats adjacent to 6 copies of the ISce1 restriction site
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    no promoter

    Available since

    Oct. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge